Опубликована: Дек. 10, 2024
Язык: Английский
CABI eBooks, Год журнала: 2025, Номер unknown, С. 17 - 34
Опубликована: Март 21, 2025
Язык: Английский
Процитировано
0
New Phytologist, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Genome editing for the development of improved varieties is supported by possibility segregating out editor T-DNA cassette after genome in many crop species. Removal prevents potential continuous activity transformed plant and furthermore facilitates regulatory approval. While transgene outcrossing exogenous sequences possible crops, this not case vegetatively propagated sterile such as Cavendish bananas. Therefore, gene techniques leading to transgene-free edited plants are essential untap those crops. Here, we present a method banana (Musa spp.) through co-editing strategy. A novel Agrobacterium tumefaciens-mediated approach combining embryogenesis chlorsulfuron selection was established validated whole sequencing. Editing acetolactate synthase (MaALS) genes using base allows effective plants. Moreover, plantlets were regenerated with mutations at two target sites, indicating that strategy can be used multiple genomic sites. The presented efficient represents first report
Язык: Английский
Процитировано
0
Journal of Experimental Botany, Год журнала: 2024, Номер 75(14), С. 4373 - 4393
Опубликована: Июнь 13, 2024
Abstract Animals and plants have developed resilience mechanisms to effectively endure overcome physical damage environmental challenges throughout their life span. To sustain vitality, both animals employ replenish damaged cells, either directly, involving the activity of adult stem or indirectly, via dedifferentiation somatic cells that are induced revert a cell state subsequently redifferentiate. Stem research has been rapidly advancing field in animal studies for many years, driven by its promising potential human therapeutics, including tissue regeneration drug development. A major breakthrough was discovery pluripotent (iPSCs), which reprogrammed from expressing limited set transcription factors. This enabled generation an unlimited supply can be differentiated into specific types tissues. Equally, keen interest connection between plant last decade, demand enhance traits such as yield, resistance pathogens, opportunities provided CRISPR/Cas-mediated gene editing. Here we discuss how knowledge biology benefits technology, speculate on creation universal genotype-independent iPSC system regenerative recalcitrance.
Язык: Английский
Процитировано
2
Advanced Materials, Год журнала: 2024, Номер 36(41)
Опубликована: Авг. 16, 2024
Abstract Delivery of proteins in plant cells can facilitate the design desired functions by modulation biological processes and traits but is currently limited narrow host range, tissue damage, poor scalability. Physical barriers plants, including cell walls membranes, limit protein delivery to tissues. Herein, a cationic high aspect ratio polymeric nanocarriers (PNCs) platform developed enable efficient plants. The nature PNCs binds through electrostatic. ability precisely PNCs’ size allowed us find cutoff ≈14 nm wall, below which autonomously overcome barrier carry their cargo into cells. To exploit these findings, reduction‐oxidation sensitive green fluorescent (roGFP) deployed as stress sensor model Nicotiana benthamiana common crop tomato maize. In vivo imaging PNC‐roGFP enabled optical monitoring response wounding, biotic, heat stressors. These results show that be designed exclusion current limitations plants species‐independent engineering.
Язык: Английский
Процитировано
2
Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер 134, С. 102402 - 102402
Опубликована: Сен. 12, 2024
Язык: Английский
Процитировано
2
Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11
Опубликована: Окт. 27, 2023
Several regions in the world are currently holding discussions regard to regulation of new genomic techniques (NGTs) and their application agriculture. The European Commission, for instance, is proposing introduction specific NGT plants. Various questions need be answered including e.g., extent which NGT-induced intended unintended genetic modifications must subjected a mandatory risk assessment as part an approval procedure. This review mostly focuses on findings changes that can caused by NGTs. More specifically, deals with nuclease CRISPR/Cas, most important tool developing plants, its potential introduce double strand breaks (DSBs) at targeted DNA sequence. For this purpose, we identified differences comparison non-targeted mutagenesis methods used conventional breeding. concludes processes relevant assessment. Due technical characteristics NGTs, sites changes, context frequency (in sites) mean resulting gene combinations (intended or unintended) may unlikely occur methods. This, turn, implies biological effects (phenotypes) also different cause risks health environment. Therefore, conclude well should comprehensive molecular characterisation plants meant environmental releases market authorisation.
Язык: Английский
Процитировано
5
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11792 - 11792
Опубликована: Ноя. 2, 2024
Forests worldwide are under increasing pressure from climate change and emerging diseases, threatening their vital ecological economic roles. Traditional breeding approaches, while valuable, inherently slow limited by the long generation times existing genetic variation of trees. CRISPR technologies offer a transformative solution, enabling precise efficient genome editing to accelerate development climate-resilient productive forests. This review provides comprehensive overview applications in forestry, exploring its potential for enhancing disease resistance, improving abiotic stress tolerance, modifying wood properties, accelerating growth. We discuss mechanisms various systems, including base editing, prime multiplexing strategies. Additionally, we highlight recent advances overcoming key challenges such as reagent delivery plant regeneration, which crucial successful implementation also delve into ethical considerations using gene drive population-level alterations, well importance containment strategies mitigating risks. emphasizes need continued research, technological advancements, extensive long-term field trials, public engagement, responsible innovation fully harness power shaping sustainable future
Язык: Английский
Процитировано
0
Plant Gene, Год журнала: 2024, Номер unknown, С. 100471 - 100471
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
0
Опубликована: Дек. 10, 2024
Язык: Английский
Процитировано
0