Efficient Agrobacterium-Mediated Methods for Transient and Stable Transformation in Common and Tartary Buckwheat DOI Open Access
Sara Leite Dias, Paride Rizzo, John C. D’Auria

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4425 - 4425

Опубликована: Май 6, 2025

Buckwheat is a promising crop with grains that are rich in nutrients and bioactive compounds. Genome sequence data for common Tartary buckwheat have recently become available. Currently, there critical need the development of simple reliable transient gene expression protocol, as well stable genetic transformation method, to facilitate metabolic engineering compounds, functional analysis genes, targeted editing, and, long-term perspective, accelerate breeding process buckwheat. In this paper, we report optimized methods Agrobacterium-mediated Fagopyrum esculentum F. tartaricum. Leaf cotyledon tissues were infiltrated an A. tumefaciens-bearing construct containing eGFP GUS reporter genes. Histochemical staining Western blotting used confirm proteins. We also demonstrate usefulness developed method gramine biosynthetic pathway HvAMIS HvNMT genes transiently expressed leaves, de novo production was confirmed by LC-MS. Moreover, planta (eGFP) selectable marker (NptII) achieved vacuum infiltration. Genomic integration polymerase chain reaction (PCR), whereas fluorescence microscopy.

Язык: Английский

Efficient Agrobacterium-Mediated Methods for Transient and Stable Transformation in Common and Tartary Buckwheat DOI Open Access
Sara Leite Dias, Paride Rizzo, John C. D’Auria

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4425 - 4425

Опубликована: Май 6, 2025

Buckwheat is a promising crop with grains that are rich in nutrients and bioactive compounds. Genome sequence data for common Tartary buckwheat have recently become available. Currently, there critical need the development of simple reliable transient gene expression protocol, as well stable genetic transformation method, to facilitate metabolic engineering compounds, functional analysis genes, targeted editing, and, long-term perspective, accelerate breeding process buckwheat. In this paper, we report optimized methods Agrobacterium-mediated Fagopyrum esculentum F. tartaricum. Leaf cotyledon tissues were infiltrated an A. tumefaciens-bearing construct containing eGFP GUS reporter genes. Histochemical staining Western blotting used confirm proteins. We also demonstrate usefulness developed method gramine biosynthetic pathway HvAMIS HvNMT genes transiently expressed leaves, de novo production was confirmed by LC-MS. Moreover, planta (eGFP) selectable marker (NptII) achieved vacuum infiltration. Genomic integration polymerase chain reaction (PCR), whereas fluorescence microscopy.

Язык: Английский

Процитировано

0