Regulatory Mechanisms of bHLH Transcription Factors in Plant Adaptive Responses to Various Abiotic Stresses

Frontiers in Plant Science, Год журнала: 2021, Номер 12

Опубликована: Июнь 18, 2021

Basic helix-loop-helix proteins (bHLHs) comprise one of the largest families transcription factors in plants. They have been shown to be involved responses various abiotic stresses, such as drought, salinity, chilling, heavy metal toxicity, iron deficiency, and osmotic damages. By specifically binding

Язык: Английский

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Transcriptional and Post-Translational Regulation of Plant bHLH Transcription Factors during the Response to Environmental Stresses

Plants, Год журнала: 2023, Номер 12(11), С. 2113 - 2113

Опубликована: Май 26, 2023

Over the past decades, extensive research has been conducted to identify and characterize various plant transcription factors involved in abiotic stress responses. Therefore, numerous efforts have made improve tolerance by engineering these factor genes. The basic Helix–Loop–Helix (bHLH) family represents one of most prominent gene families contains a bHLH motif that is highly conserved eukaryotic organisms. By binding specific positions promoters, they activate or repress response genes thus affect multiple variables physiology such as stresses, which include drought, climatic variations, mineral deficiencies, excessive salinity, water stress. regulation crucial better control their activity. On hand, are regulated at transcriptional level other upstream components; on undergo modifications ubiquitination, phosphorylation, glycosylation post-translational level. Modified can form complex regulatory network regulate expression determine activation physiological metabolic reactions. This review article focuses structural characteristics, classification, function, mechanism levels during responses conditions.

Язык: Английский

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LcbHLH107 actively suppresses the expression of LcDFR to repress anthocyanin biosynthesis in Litchi chinensis Sonn.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142396 - 142396

Опубликована: Март 1, 2025

Язык: Английский

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Genome-Wide Identification and Comprehensive Analysis of the AP2/ERF Gene Family in Pomegranate Fruit Development and Postharvest Preservation

Genes, Год журнала: 2022, Номер 13(5), С. 895 - 895

Опубликована: Май 17, 2022

Pomegranate (Punica granatum L.) is a kind of fruit with significant economic, ecological and health values. AP2/ERF transcription factors belong to large group mainly found in plants play key roles plant growth development. However, genes pomegranate their implication development postharvest preservation have been little described. In this study, 116 PgAP2/ERF were identified renamed based on chromosomal distributions. Phylogenetic relationship from other species, structures, duplications, annotations, cis-elements promoter sequences, protein-protein interaction networks among proteins comprehensively explored. Expression analysis revealed several PgAP2/ERFs associated the phenotypes seed hardness, including PgAP2/ERF5, PgAP2/ERF36, PgAP2/ERF58, PgAP2/ERF86. Subsequent indicated that many differentially expressed are potentially important regulators Furthermore, expression more than one-half was repressed ‘Tunisian soft seed’ under low-temperature cold storage. The results showed 1-MCP implicated promoting upregulated PgAP2/ERF4, PgAP2/ERF15, PgAP2/ERF26, PgAP2/ERF30, PgAP2/ERF35 PgAP2/ERF45 compared those This indicates these candidate involved preservation. summary, findings present study provide an basis for characterizing family information

Язык: Английский

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Systematic Analysis of bHLH Transcription Factors in Cassava Uncovers Their Roles in Postharvest Physiological Deterioration and Cyanogenic Glycosides Biosynthesis

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Июнь 16, 2022

The basic helix-loop-helix (bHLH) proteins are a large superfamily of transcription factors, and play central role in wide range metabolic, physiological, developmental processes higher organisms. However, systematic investigation bHLH gene family cassava (Manihot esculenta Crantz) has not been reported. In the present study, we performed genome-wide survey identified 148 MebHLHs genes were unevenly harbored 18 chromosomes. Through phylogenetic analyses along with Arabidopsis counterparts, these divided into 19 groups, each contains similar structure conserved motifs. Moreover, many cis-acting regulatory elements related to various defense stress responses showed MebHLH genes. Interestingly, transcriptome data unveiled 117 during postharvest physiological deterioration (PPD) process tuberous roots, while 65 significantly change. Meanwhile, relative quantitative analysis 15 demonstrated that they sensitive PPD, suggesting may involve PPD regulation. Cyanogenic glucosides (CGs) biosynthesis was increased, silencing MebHLH72 MebHLH114 linamarin content decreased leaves. To summarize, identification expression profiling candidates pave new avenue for uderstanding their function CGs biosynthesis, which will accelerate improvement tolerance decrease roots.

Язык: Английский

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Genome-wide identification and characterization of PdbHLH transcription factors related to anthocyanin biosynthesis in colored-leaf poplar (Populus deltoids)

BMC Genomics, Год журнала: 2022, Номер 23(1)

Опубликована: Март 28, 2022

Abstract Basic helix-loop-helix (bHLH) proteins are transcription factors (TFs) that have been shown to regulate anthocyanin biosynthesis in many plant species. However, the bHLH gene family Populus deltoids has not yet reported. In this study, 185 PdbHLH genes were identified genome and classified into 15 groups based on their sequence similarity phylogenetic relationships. Analysis of structure, chromosome location conserved motif performed by bioinformatic methods. Gene duplication analyses revealed 114 expanded retained after WGD/segmental proximal duplication. Investigation cis-regulatory elements indicated involved regulation biosynthesis. The expression patterns PdbHLHs obtained from previous data two colored-leaf poplar (QHP JHP) green leaf (L2025). Further analysis 12 candidate genes, including 3 ( PdbHLH57 , PdbHLH143 PdbHLH173 ) subgroup III(f) 9 other groups, positively associated with addition, 4 PdbHLH4 PdbHLH1 PdbHLH18 PdbHLH164 may be negatively regulating These results provide a basis for functional characterization investigations molecular mechanisms poplar.

Язык: Английский

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Genome-Wide Identification and Low-Temperature Expression Analysis of bHLH Genes in Prunus mume

Frontiers in Genetics, Год журнала: 2021, Номер 12

Опубликована: Окт. 1, 2021

Prunus mume is an illustrious ornamental woody plant with colorful flowers, delicate fragrances, and graceful tree forms. Low temperature limits its geographical distribution. The basic helix-loop-helix (bHLH) proteins exist in most eukaryotes as a transcription factor superfamily, which play crucial role metabolism, physiology, development, response to various stresses of higher organisms. However, the characteristics bHLH gene family low-temperature remain unknown P. . In present study, we distinguished 95 PmbHLH genes whole-genome analyzed their features. PmbHLHs were divided into 23 subfamilies one orphan by phylogenetic analysis. Similar structures conserved motifs appeared same subfamily. These situated eight chromosomes scaffolds. Gene duplication events performed close relationship , persica, avium Tandem duplications probably promoted expansion According predicted binding activities, defined Non-DNA-binding DNA-binding proteins. Furthermore, exhibited tissue-specific induced expression patterns. By analyzing transcriptome data, 10 are responsive stress selected. qRT-PCR results showed that ten could respond at different degrees. There differences multiple variations among varieties. This study provides basis research evolution tolerance might enhance breeding programs improving tolerance.

Язык: Английский

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Genome-Wide Characterization and Analysis of bHLH Transcription Factors Related to Anthocyanin Biosynthesis in Cinnamomum camphora (‘Gantong 1’)

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 3498 - 3498

Опубликована: Фев. 9, 2023

Cinnamomum camphora is one of the most commonly used tree species in landscaping. Improving its ornamental traits, particularly bark and leaf colors, key breeding goals. The basic helix-loop-helix (bHLH) transcription factors (TFs) are crucial controlling anthocyanin biosynthesis many plants. However, their role C. remains largely unknown. In this study, we identified 150 bHLH TFs (CcbHLHs) using natural mutant 'Gantong 1', which has unusual colors. Phylogenetic analysis revealed that CcbHLHs were divided into 26 subfamilies shared similar gene structures conserved motifs. According to protein homology analysis, four candidate highly compared TT8 A. thaliana. These potentially involved camphora. RNA-seq specific expression patterns different tissue types. Furthermore, verified seven (CcbHLH001, CcbHLH015, CcbHLH017, CcbHLH022, CcbHLH101, CcbHLH118, CcbHLH134) various types at growth stages qRT-PCR. This study opens a new avenue for subsequent research on regulated by CcbHLH

Язык: Английский

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Genome-wide Analysis of Basic Helix-Loop-Helix Family Genes and Expression Analysis in Response to Drought and Salt Stresses in Hibiscus hamabo Sieb. et Zucc

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(16), С. 8748 - 8748

Опубликована: Авг. 15, 2021

The basic helix-loop-helix (bHLH) family of transcription factors is one the most significant and biggest in plants. It involved regulation both growth development, as well stress response. Numerous members bHLH have been found characterized woody plants recent years. However, no systematic study gene has published for Hibiscus hamabo Sieb. et Zucc. In this research, we identified 162 proteins (HhbHLHs) from genomic transcriptomic datasets H. hamabo, which were phylogenetically divided into 19 subfamilies. According to a structural study, number exon-introns HhbHLHs varied between zero seventeen. MEME research revealed that majority HhbHLH contained three conserved motifs, 1, 4, 5. examination promoter cis-elements genes had several plant development abiotic responses. addition, overexpression HhbHLH2 increased salt drought tolerance Arabidopsis.

Язык: Английский

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Identification, Characterization and Expression Analysis of Anthocyanin Biosynthesis-related bHLH Genes in Blueberry (Vaccinium corymbosum L.)

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(24), С. 13274 - 13274

Опубликована: Дек. 10, 2021

Basic helix-loop-helix proteins (bHLHs) play very important roles in the anthocyanin biosynthesis of many plant species. However, reports on blueberry biosynthesis-related bHLHs were limited. In this study, six identified from genome data through homologous protein sequence alignment. Among these bHLHs, VcAN1, VcbHLH42-1, VcbHLH42-2 and VcbHLH42-3 clustered into one group, while VcbHLH1-1 VcbHLH1-2 other group. All bHLH-MYC_N domain, had DNA binding sites reported conserved amino acids bHLH indicating that they all G-box proteins. Protein subcellular location prediction result revealed nucleus-located. Gene structure analysis showed VcAN1 gDNA contained eight introns, others seven introns. Many light-, phytohormone-, stress- growth development-related cis-acting elements transcription factor (TFBSs) their promoters, but types numbers cis-elements TFBSs varied greatly between two groups. Quantitative real-time PCR results expressed highly old leaf, stem blue fruit, its expression increased as fruit ripened. Its purple podetium leaf was respectively significantly higher than green young plays regulation not only also leaf. highest stem, lowest fruit. The ripened, high low especially red Our study indicated regulatory functions certain spatiotemporal specificity. Additionally, might be a key gene controlling blueberry, whose function is worth exploring further for potential applications breeding.

Язык: Английский

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