Mechanisms of Intrinsic Postzygotic Isolation: From Traditional Genic and Chromosomal Views to Genomic and Epigenetic Perspectives

Cold Spring Harbor Perspectives in Biology, Год журнала: 2023, Номер 15(10), С. a041607 - a041607

Опубликована: Сен. 11, 2023

Radka Reifová1, S. Lorena Ament-Velásquez2, Yann Bourgeois3, Jenn Coughlan4, Jonna Kulmuni5,6, Agnieszka P. Lipinska7,8, Genta Okude9, Laurie Stevison10, Kohta Yoshida9 and Jun Kitano9 1Department of Zoology, Faculty Science, Charles University, 128 00 Prague, Czech Republic 2Department Stockholm 106 91 Stockholm, Sweden 3DIADE, University Montpellier, CIRAD, IRD, 34090 France 4Department Ecology & Evolutionary Biology, Yale New Haven, Connecticut 06520, USA 5Institute for Biodiversity Ecosystem Dynamics, Department Population Amsterdam, 1012 The Netherlands 6Organismal Biology Research Programme, Helsinki, 00100 Finland 7Department Algal Development Evolution, Max Planck Institute 72076 Tuebingen, Germany 8CNRS, UMR 8227, Integrative Marine Models, Sorbonne Université, Station Biologique de Roscoff, 29680 9Department Genomics National Genetics, Mishima, Shizuoka 411-8540, Japan 10Department Biological Sciences, Auburn Auburn, Alabama 36849, Correspondence: radka.reifova{at}natur.cuni.cz; jkitano{at}nig.ac.jp

Язык: Английский

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The regulatory basis of migratory behaviour in birds: different paths to similar outcomes

Journal of Avian Biology, Год журнала: 2024, Номер unknown

Опубликована: Июнь 10, 2024

Animal migration is a fascinating phenomenon that has puzzled mankind since the time of ancient Greece. It process widespread across varied range taxa and it shines especially in birds which, because their mobility, display an amazing diversity routes strategies. With advances tracking devices improvements sequencing technologies, recent work provides support for strong genetic influence several migratory traits different species. However, there little to no evidence any common sequence‐based mechanism behind this complex behaviour, nor unifying principle explaining it. We review how focus understanding basis should be shifted towards studying regulatory mechanisms gene expression instead traditional candidate approach. Importantly, role as underlying driver phenotype can resolve opposing often views mainly either under or environmental influence. emphasise research take new directions, reinforcing probably not regulated birds. Here, we notion 1) only evolve fast if quantitative trait with large standing variation; 2) main drivers evolution seem diverse expression–regulation rather than gene‐level polymorphisms; 3) non‐coding sequences genome, epigenetics structural variation might more important shaping previously thought. Further, present hypotheses outlining these bird species defining certain traits.

Язык: Английский

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Mendelian nightmares: the germline-restricted chromosome of songbirds

Chromosome Research, Год журнала: 2022, Номер 30(2-3), С. 255 - 272

Опубликована: Апрель 13, 2022

Abstract Germline-restricted chromosomes (GRCs) are accessory that occur only in germ cells. They eliminated from somatic cells through programmed DNA elimination during embryo development. GRCs have been observed several unrelated animal taxa and show peculiar modes of non-Mendelian inheritance within-individual elimination. Recent cytogenetic phylogenomic evidence suggests a GRC is present across the species-rich songbirds, but absent non-passerine birds, implying over half all 10,500 bird species extensive germline/soma genome differences. Here, we review recent insights gained genomic, transcriptomic, approaches with regard to genetic content, phylogenetic distribution, songbird GRC. While many questions remain unsolved terms inheritance, elimination, function, discuss plausible scenarios future directions for understanding this widespread form

Язык: Английский

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Rapid gene content turnover on the germline-restricted chromosome in songbirds

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Июль 29, 2023

The germline-restricted chromosome (GRC) of songbirds represents a taxonomically widespread example programmed DNA elimination. Despite its apparent indispensability, we still know very little about the GRC's genetic composition, function, and evolutionary significance. Here assemble GRC in two closely related species, common thrush nightingale. In total identify 192 genes across GRCs, with many them present multiple copies. Interestingly, appears to be under selective pressure, content differing dramatically between species appearing pseudogenized fragments. Only one gene, cpeb1, has complete coding region all examined individuals shows no copy number variation. acquisition this gene by corresponds earliest estimates origin, making it good candidate for functional indispensability songbirds.

Язык: Английский

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Micro Germline-Restricted Chromosome in Blue Tits: Evidence for Meiotic Functions

Molecular Biology and Evolution, Год журнала: 2023, Номер 40(5)

Опубликована: Апрель 28, 2023

Abstract The germline-restricted chromosome (GRC) is likely present in all songbird species but differs widely size and gene content. This extra has been described as either a microchromosome with only limited basic content or macrochromosome enriched functions related to female gonad embryo development. Here, we assembled, annotated, characterized the first micro-GRC blue tit (Cyanistes caeruleus) using high-fidelity long-read sequencing data. Although some genes on GRC show signals of pseudogenization, others potentially have important functions, currently past. We highlight paralog BMP15, which among highest expressed both tits zebra finches (Taeniopygia guttata) known play role oocyte follicular maturation other vertebrates. are further for synaptonemal complex. found similar functional enrichment when analyzing published data from two nightingale (Luscinia spp.). hypothesize that these maintaining standard maternal inheritance recombining paternal GRCs during potential episodes biparental inheritance.

Язык: Английский

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Premeiotic endoreplication is the mechanism of obligate parthenogenesis in rock lizards of the genusDarevskia

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 1, 2024

Abstract Among vertebrates, obligate parthenogenesis occurs exclusively in squamate reptiles. Premeiotic endoreplication a small subset of developing oocytes has been documented as the mechanism production unreduced eggs minutely explored parthenogenetic lineages, namely teiids and geckos. The situation lacertid genus Darevskia discussed for decades. Certain observations suggested that ploidy level is restored during egg formation through fusion polar body nuclei D. unisexualis armeniaca . In this study, we re-evaluated hypothesis by studying diplotene chromosomes adult females sexual species raddei nairensis parthenogens armeniaca, dahli We revealed 19 bivalents 38 diploid parthenogens, which uncovers premeiotic non-reduced females. earlier contradicting reports can be likely attributed to difficulty identifying mispairing pachytene, fact reptiles relying on only undergo genome doubling overcome pachytene checkpoint. This study highlights co-option escape from reproduction all independent hybrid origins vertebrates studied date.

Язык: Английский

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Germline-restricted chromosome shows remarkable variation in size among closely related passerine species

Chromosoma, Год журнала: 2022, Номер 131(1-2), С. 77 - 86

Опубликована: Апрель 7, 2022

Язык: Английский

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Karyotypes of Manatees: New Insights into Hybrid Formation (Trichechus inunguis × Trichechus m. manatus) in the Amazon Estuary

Genes, Год журнала: 2022, Номер 13(7), С. 1263 - 1263

Опубликована: Июль 16, 2022

Great efforts have been made to preserve manatees. Recently, a hybrid zone was described between Trichechus inunguis (TIN) and the manatus (TMM) in Amazon estuary. Cytogenetic data on these sirenians are limited, despite being fundamental understanding hybridization/introgression dynamics genomic organization Trichechus. We analyzed karyotype of TMM, TIN, two specimens ("Poque" "Vitor") by classical molecular cytogenetics. G-band analysis revealed that TMM (2n = 48) TIN 56) diverge at least six Robertsonian translocations pericentric inversion. Hybrids had 2n 50, however, with Autosomal Fundamental Number (FNA) 88 "Poque" FNA 74 "Vitor", chromosomal distinct pairs heterozygous; additionally, "Vitor" exhibited heteromorphisms chromosomes whose could not be determined. The U2 snDNA Histone H3 multi genes distributed small clusters along transposable Keno Helitron elements (TEs) their sequences. different karyotypes observed among manatee hybrids may indicate they represent generations formed crossing fertile TIN. On other hand, it is also possible all recorded F1 differences must result from mechanisms elimination.

Язык: Английский

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Direct Chromosome Preparation Method in Avian Embryos for Cytogenetic Studies: Quick, Easy and Cheap

DNA, Год журнала: 2022, Номер 2(1), С. 22 - 29

Опубликована: Янв. 26, 2022

Avian cell culture is widely applied for cytogenetic studies, the improvement of which increasingly allows production high-quality chromosomes, essential to perform both classical and molecular studies. Among these approaches, there are two main types: fibroblast bone marrow culture. Despite its high cost complexity, considered superior approach due quality metaphases produced. Short-term cultivation provides more condensed chromosomes but nonetheless quicker easier. In search a quicker, cheaper way prepare without losing quality, present work developed novel, applicable protocol avian chromosome preparation. Twenty-one bird embryos from distinct families were sampled: Icteridae, Columbidae, Furnariidae, Estrildidae, Thraupidae, Troglodytidae Ardeidae. The was based on combination modified cultivation, taking advantages both. results show that all species consistently presented good mitotic indexes chromosomes. Overall, application this cytogenetics can optimize time, considering most cultures take at least 3 days often much longer. However, our be performed in h with much-reduced reagents equipment.

Язык: Английский

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Germline-restricted chromosomes of the songbirds

Vavilov Journal of Genetics and Breeding, Год журнала: 2023, Номер 27(6), С. 641 - 650

Опубликована: Ноя. 2, 2023

Germline-restricted chromosomes (GRCs) are present in the genomes of germline cells and absent from somatic cells. A GRC is found all species songbirds (Passeri) none other bird orders studied to date. This indicates that originated common ancestor songbirds. The germline-restricted chromosome permanently songbird, while female usually contain two copies male ones have one copy. In females, GRCs undergo synapsis restricted recombination their terminal regions during meiotic prophase. males, it almost always eliminated spermatocytes. Thus, inherited exclusively through maternal lineage. a necessary genomic element To date, genetic composition has been four only. Some genes actively expressed gonads, controlling development synthesis proteins involved organization chromosomes. Songbird vary size composition. each consists amplified modified basic genome species. level homology between different relatively low, indicating high rate evolution this chromosome. Transmission lineage suppression contribute significantly accelerated GRCs. One may suggest rapid coordinated might be responsible for explosive speciation adaptive radiation most species-rich diverse infraorder birds.Хромосомы, ограниченные зародышевой линией (germline-restricted – GRC), присутствуют в геномах герминативных клеток и запрограммированно элиминируются из соматических ходе развития. Они крайне редко возникают эволюции. Еще реже они становятся необходимыми элементами геномов крупных таксонов. Такая хромосома, ограниченная линией, была обнаружена у всех исследованных представителей подотряда воробьинообразных певчих птиц. Ни одного других отрядов птиц не выявлено. Это свидетельствует о том, что возникла общего предка У этого присутствует, как правило, двух копиях клетках самок одной копии самцов отсутствует клетках. синаптируют рекомбинируют профазе мейоза. почти всегда сперматоцитов. Таким образом, наследуется исключительно по материнской линии. Хромосомы, необходимый элемент генома На сегодняшний день исследованы геномы четырех видов. Некоторые гены активно экспрессируются гонадах самок, контролируют развитие клеток, синтез белков, вовлеченных организацию мейотических хромосом. разных видов различаются размерам генетическому составу. Геном каждого вида состоит амплифицированных перестроенных копий генов основного данного вида. Уровень гомологии между довольно низок. указывает на высокую скорость эволюции генетического состава хромосомы. Значительный вклад ускорение вносят передача этой хромосомы линии подавление рекомбинации ней. Можно считать, быстрая согласованная эволюция набора играет важную роль видообразовании адаптивной радиации самого богатого видами разнообразного

Язык: Русский

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