The
multidisciplinary
approach
in
the
management
of
polytrauma
patients
is
utmost
importance
for
proper
evaluation
and
treatment
injuries
different
body
systems,
as
second
most
common
cause
death
these
occurs
due
to
treatable
injuries.The
aim
this
article
highlight
correct
through
a
review
current
literature.In
article,
we
present
literature
widely
used
systematic
approach.We
discuss
ABCDE
sequence
ATLS
protocol,
prioritizing
airway
control,
breathing
assessment,
circulation
hemorrhage
neurological
patient
exposure
injury
identification.After
stabilization
identified
injuries,
detailed
secondary
assessment
crucial.The
emphasizes
specialized
team
provide
care
optimize
outcomes
complex
clinical
situation.
Insects,
Год журнала:
2024,
Номер
15(11), С. 870 - 870
Опубликована: Ноя. 6, 2024
Rare
and
ultra-rare
diseases
constitute
a
significant
medical
challenge
due
to
their
low
prevalence
the
limited
understanding
of
origin
underlying
mechanisms.
These
disorders
often
exhibit
phenotypic
diversity
molecular
complexity
that
represent
biomedical
research.
There
are
more
than
6000
different
rare
affect
nearly
300
million
people
worldwide.
However,
each
disease
is
low,
in
consequence,
resources
dedicated
insufficient
effectively
achieve
progress
The
use
animal
models
investigate
mechanisms
pathogenesis
has
become
an
invaluable
tool.
Among
commonly
used
research,
G3 Genes Genomes Genetics,
Год журнала:
2023,
Номер
13(5)
Опубликована: Март 6, 2023
Abstract
Received
on
16
January
2023;
accepted
21
February
2023Kinases
are
key
regulators
of
cellular
signal
transduction
pathways.
Many
diseases,
including
cancer,
associated
with
global
alterations
in
protein
phosphorylation
networks.
As
a
result,
kinases
frequent
targets
drug
discovery
efforts.
However,
target
identification
and
assessment,
critical
step
targeted
that
involves
identifying
essential
genetic
mediators
disease
phenotypes,
can
be
challenging
complex,
heterogeneous
diseases
like
where
multiple
concurrent
genomic
common.
Drosophila
is
particularly
useful
model
system
to
identify
novel
biological
processes
through
unbiased
screens.
Here,
we
report
2
classic
modifier
screens
focusing
the
kinome
kinase
different
backgrounds:
KRAS
TP53
PTEN
APC,
multigenic
cancer
4
genes
recurrently
mutated
human
colon
tumors
alone,
simpler
one
most
frequently
altered
pathways
cancer.
These
identified
hits
unique
each
shared
by
both,
emphasizing
importance
capturing
complexity
tumor
genome
landscapes
experimental
models.
Our
follow-up
analysis
from
KRAS-only
screen
suggests
classical
heterozygous
mutant
backgrounds
result
modest,
nonlethal
reduction
candidate
gene
activity
context
whole
animal—a
goal
systemic
treatment—may
approach
rate-limiting
vulnerabilities
models
as
ideal
targets.
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 6, 2024
Given
the
increasing
concern
about
antimicrobial
resistance
among
microorganisms
that
cause
infections
in
our
society,
there
is
an
urgent
need
for
new
drug
discovery.
Currently,
this
process
involves
testing
many
low-quality
compounds,
resulting
from
vivo
testing,
on
mammal
models,
which
not
only
wastes
time,
resources,
and
money,
but
also
raises
ethical
questions.
In
review,
we
have
discussed
potential
of
D.
melanogaster
as
intermediary
experimental
model
discovery
timeline.
We
tackled
topic
a
methodological
perspective,
providing
recommendations
regarding
range
concentrations
to
test
based
mechanism
action
each
compound;
how
treat
,
monitor
treatment,
what
parameters
should
consider
when
designing
screening
protocol
maximize
study’s
benefits.
discuss
necessary
improvements
needed
establish
infection
standard
technique
process.
Overall,
has
been
demonstrated
be
manageable
studying
broad-spectrum
treatment.
It
allows
us
obtain
valuable
information
cost-effective
manner,
can
improve
provide
insights
into
current
major
concern.
This
approach
line
with
3R
policy
biomedical
research,
particular
replacement
reduce
use
vertebrates
preclinical
development.
Cancers,
Год журнала:
2023,
Номер
15(19), С. 4721 - 4721
Опубликована: Сен. 26, 2023
Zebrafish
is
increasingly
used
as
a
model
organism
for
cancer
research
because
of
its
genetic
and
physiological
similarities
to
humans.
Modeling
lung
(LC)
in
zebrafish
has
received
significant
attention.
This
review
focuses
on
the
insights
gained
from
using
LC
research.
These
range
investigating
molecular
mechanisms
that
contribute
development
progression
identifying
potential
drug
targets,
testing
efficacy
toxicity
new
therapies,
applying
personalized
medicine
studies.
provides
comprehensive
overview
current
state
performed
zebrafish,
highlights
advantages
limitations
this
organism,
discusses
future
directions
field.
Drosophila
melanogaster
serves
as
a
important
research
model
both
for
genetic
investigations
and
the
study
of
metal
toxicity,
facilitating
elucidation
physiological
mechanisms
comparable
to
those
human
organisms.
In
our
research,
we
evaluated
toxicity
effect
resulting
from
exposure
various
concentrations
lead
copper
on
prolificity
rates
throughout
life
cycle
(egg-adult)
four
genotypes
melanogaster:
wild-type
(control),
white,
brown,
white-vestigial.
During
study,
were
examined
across
three
repetitions
under
impact
0.50,
1.00,
2.00,
4.00
mM
(CuSO4)
(Pb(C2H3O2)2).
Prolificity
exhibited
variations
direct
consequence
factors,
concentration
exposure,
specific
type
metal,
either
or
lead.
The
mutant
white-vestigial
genotype
revealed
an
IC50
inhibition
at
lower
doses
2.00
lead,
in
contrast
control
(wild-type),
which
rate
>IC50
only
case
copper.
Our
results
concluded
that
(i)
dose
influences
directly
proportional
manner,
(ii)
comparative
analyses
between
displayed
all
within
range
0.50
mM.
contrast,
1.00
mM,
highlighting
(iii)
more
acute
characteristic
Thus,
this
reflect
importance
using
interaction
factors
metals,
offering
significant
insights
into
monitoring
their
defining
maximum
permissible
Frontiers in Bioscience-Landmark,
Год журнала:
2024,
Номер
29(6), С. 234 - 234
Опубликована: Июнь 25, 2024
Drosophila
melanogaster
has
been
used
as
a
model
system
to
identify
and
characterize
genetic
contributions
development,
homeostasis,
investigate
the
molecular
determinants
of
numerous
human
diseases.
While
there
exist
many
differences
at
genetic,
structural,
level,
signalling
components
cellular
machineries
are
conserved
between
humans.
For
this
reason,
can
extensively
model,
study
pathologies.
The
extensive
resources
available
make
powerful
one.
Over
years,
sophisticated
rapidly
expanding
toolkit
provided
valuable
novel
insights
into
contribution
activity
Notch
is
crucial
during
development
across
Metazoa
associated
with
Here
we
highlight
examples
mechanisms
involving
that
have
elucidated
from
modelling
diseases
in
include
neurodegenerative
diseases,
congenital
several
cancers,
cardiac
disorders.
Cells,
Год журнала:
2024,
Номер
13(14), С. 1192 - 1192
Опубликована: Июль 14, 2024
Studies
on
numerous
species
have
demonstrated
strikingly
conserved
mechanisms
that
determine
the
aging
process,
from
yeasts
to
worms,
flies,
zebrafish,
mice,
and
humans.
The
fruit
fly
Drosophila
melanogaster
is
an
excellent
model
organism
for
studying
biological
basis
of
normal
etiology
age-related
diseases.
Since
its
inception
in
1967,
Bloomington
Stock
Center
(BDSC)
has
grown
into
largest
collection
documented
D.
strains
(currently
>
91,000).
This
paper
aims
briefly
review
provides
a
guide
help
users
understand
organization
stock
listings
BDSC
website
familiarize
themselves
with
search
functions
FlyBase,
emphasis
using
genes
pathways
as
examples
find
stocks
studies.
Frontiers in Cell Death,
Год журнала:
2024,
Номер
3
Опубликована: Ноя. 26, 2024
Cell
death
is
an
essential
physiological
process
for
the
survival
of
multicellular
organisms.
Our
understanding
programmed
cell
in
development,
immune
function
maintenance,
and
adult
tissue
repair
has
significantly
advanced
over
past
decade.
However,
there
are
still
gaps
our
knowledge
about
induction,
regulation,
checkpoints
this
due
to
diverse
forms
cellular
suicide
rapid
nature
process.
Molecular
advancements
such
as
specific
sensors,
RNA-seq,
single-cell
proteomics
have
allowed
identifying
new
factors
a
better
molecular
networks
pathways
that
regulate
these
processes.
Programmed
also
plays
role
cancer,
both
limiting
facilitating
aspects
malignant
process,
making
its
analysis
inhibition
challenging.
This
review
discusses
field’s
using
model
organism
Drosophila
melanogaster
,
types
development
tissues,
techniques
studying
it,
cancer.
