Rabeprazole suppressed gastric intestinal metaplasia through activation of GPX4-mediated ferroptosis DOI Creative Commons
Jing Xie,

Xinhua Liang,

Fangfang Xie

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 7, 2024

Background Gastric intestinal metaplasia is a common pathological feature in patients with Helicobacter pylori ( H. ) infection. Rabeprazole was widely used as the first-line regimen for infectious treatment. The objective of this study to explore mechanism rabeprazole gastric Methods Real-time PCR, Western blotting (WB) and ROS analysis were conducted confirm that could induce ferroptosis suppress metaplasia. Cellular fraction, luciferase chromatin immunoprecipitation (ChIP) identify underlying modulated ferroptosis. Results Herein, we found treatment led inhibit CDX2 MUC2 expression, alleviating metaplasia, which attributed enhanced characterized by decreased GPX4 expression. Inhibition ferrostatin-1 (Fer-1) reverse expression caused rabeprazole. Mechanically, CREB phosphorylation nuclear translocation, further binding promoter, reducing transactivity. Moreover, endogenous PKA interacted CREB, interaction drastically destroyed response Most importantly, observed pylori- infected comparison HC control. Conclusion These findings suggested induced reduce epithelial cells through PKA/CREB cascade signaling, implying targeting be promising strategy improving during -infected patients.

Язык: Английский

Regulatory mechanisms and potential therapeutic targets in precancerous lesions of gastric cancer: A comprehensive review DOI Open Access

Maofu Zhang,

Jialin Zhong,

Zhongyang Song

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 177, С. 117068 - 117068

Опубликована: Июль 16, 2024

Precancerous lesions of gastric cancer (PLGC) represent a critical pathological stage in the transformation from normal mucosa to (GC). The global incidence PLGC has been rising over past few decades, with trend towards younger onset ages. Increasing evidence suggests that early prevention and treatment can effectively reverse malignant development mucosal epithelial cells. However, there is currently lack effective therapeutic drugs methods. Recent years have witnessed substantial advancements research, elucidation novel regulatory mechanisms offering promising avenues for clinical intervention drug development. This review aims delineate potential targets diagnosis GC while exploring innovative approaches management. article focuses on elucidating inflammatory microenvironment, bile acids (BA), glycolysis, autophagy, apoptosis, ferroptosis, cellular senescence. We pay particular attention PLGC, goal providing insights theoretical basis research PLGC.

Язык: Английский

Процитировано

5

Gastric Intestinal Metaplasia: Challenges and the Opportunity for Precision Prevention DOI Open Access
Douglas Tjandra, Rita A. Busuttil, Alex Boussioutas

и другие.

Cancers, Год журнала: 2023, Номер 15(15), С. 3913 - 3913

Опубликована: Авг. 1, 2023

GIM is a persistent, premalignant lesion whereby gastric mucosa replaced by metaplastic resembling intestinal tissue, arising in the setting of chronic inflammation, particularly context Helicobacter pylori. While overall rates progression to adenocarcinoma are low, estimated at from 0.25 2.5%, there features that confer much higher risk and warrant follow-up. In this review, we collate summarise current knowledge regarding pathogenesis GIM, clinical, endoscopic histologic factors for cancer. We examine state-of-practice with regard diagnosis management which varies widely published guidelines practice. consider emerging evidence population studies, artificial intelligence molecular markers, will guide future models care. The ultimate goal increase detection early dysplasia/neoplasia can be cured while avoiding unnecessary surveillance very low-risk individuals.

Язык: Английский

Процитировано

6

Сharacterization of the immune microenvironment’s cellular composition and its influence on gene expression during metaplastic changes of the gastric mucosa epithelium DOI Creative Commons
Iurii K. Slepov, A.M. Emelin, R. V Deev

и другие.

Morphology, Год журнала: 2024, Номер 161(4), С. 53 - 65

Опубликована: Июнь 17, 2024

BACKGROUND: Intestinal metaplasia of the gastric mucosa epithelium in chronic atrophic gastritis is considered a precancerous condition; however, it potentially reversible. The study regulation mechanisms metaplastic epithelial changes may help understanding carcinogenesis and cancer prevention. AIM: To determine whether microenvironment related to development patients with by assessing gene expression cellular composition immune infiltrates. MATERIALS AND METHODS: In this retrospective cohort study, alternative hypothesis was that differed between cases without epithelium. Biopsy specimens (n=38) obtained during endoscopic examination from five stomach sites (2 antrum, 2 body, 1 corner) unspecified etiology results RNA sequencing biopsy registered NCBI open database (n=12) were analyzed. Histological analysis, histochemical staining methods, immunohistochemical morphometric, statistical, bioinformatic analyses performed. RESULTS: proportion macrophages, T-cytotoxic lymphocytes, plasmocytes increased samples A correlation found lymphocytes risk for metaplasia. It number B cells, macrophages M2, T-regulatory cells NK-cells are associated increase six genes most specific intestinal-type CONCLUSION: significant difference mucosal indicates potential influence on progression pathological process along Correa cascade. One be their as an epigenetic factor.

Язык: Английский

Процитировано

0

Rabeprazole suppressed gastric intestinal metaplasia through activation of GPX4-mediated ferroptosis DOI Creative Commons
Jing Xie,

Xinhua Liang,

Fangfang Xie

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 7, 2024

Background Gastric intestinal metaplasia is a common pathological feature in patients with Helicobacter pylori ( H. ) infection. Rabeprazole was widely used as the first-line regimen for infectious treatment. The objective of this study to explore mechanism rabeprazole gastric Methods Real-time PCR, Western blotting (WB) and ROS analysis were conducted confirm that could induce ferroptosis suppress metaplasia. Cellular fraction, luciferase chromatin immunoprecipitation (ChIP) identify underlying modulated ferroptosis. Results Herein, we found treatment led inhibit CDX2 MUC2 expression, alleviating metaplasia, which attributed enhanced characterized by decreased GPX4 expression. Inhibition ferrostatin-1 (Fer-1) reverse expression caused rabeprazole. Mechanically, CREB phosphorylation nuclear translocation, further binding promoter, reducing transactivity. Moreover, endogenous PKA interacted CREB, interaction drastically destroyed response Most importantly, observed pylori- infected comparison HC control. Conclusion These findings suggested induced reduce epithelial cells through PKA/CREB cascade signaling, implying targeting be promising strategy improving during -infected patients.

Язык: Английский

Процитировано

0