Genome-wide identification and comprehensive analysis of the AP2/ERF gene family in Prunus sibirica under low-temperature stress
BMC Plant Biology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Сен. 28, 2024
Язык: Английский
A Comprehensive Evolutionary Analysis of the Dihydroflavonol 4-Reductase (DFR) Gene Family in Plants: Insights from 237 Species
Genes,
Год журнала:
2025,
Номер
16(4), С. 396 - 396
Опубликована: Март 29, 2025
Background:
Dihydroflavonol
4-reductase
(DFR)
is
a
key
enzyme
in
the
flavonoid
biosynthetic
pathway
that
regulates
anthocyanin
and
proanthocyanidin
accumulation
plants.
Although
DFR
genes
have
been
studied
various
species,
their
origin
of
gene
family,
its
distribution
across
plant
kingdom,
reasons
behind
emergence
different
subtypes
Methods:
This
study
performed
whole-genome
analysis
237
including
algae,
mosses,
ferns,
gymnosperms,
angiosperms,
integrating
phylogeny,
conserved
motifs,
duplication
mechanisms,
positive
selection,
expression
pattern
analyses.
Results:
These
results
indicate
family
originated
from
common
ancestor
extant
ferns
seed
plants,
asparagine
(Asn)-type
aspartic
(Asp)-type
DFRs
associated
with
gymnosperms.
Notably,
we
report
for
first
time
presence
Asn-type,
Asp-type,
arginine
(Arg)-type
some
which
breaks
previous
notion
Arg-type
are
exclusive
to
ferns.
Tandem
considered
primary
driving
force
expansion
formation
subtypes.
Furthermore,
Asn-type
were
highly
expressed
during
early
stages
development,
suggesting
important
role
development.
Conclusions:
Overall,
this
revealed
dynamic
evolutionary
trajectory
providing
theoretical
foundation
future
research
on
genes.
Язык: Английский
Integrated transcriptomics and metabolomics to explore the mechanisms of Elaeagnus mollis diels seed viability decline
BMC Genomics,
Год журнала:
2025,
Номер
26(1)
Опубликована: Апрель 2, 2025
Abstract
Elaeagnus
mollis
Diels,
is
a
rare
and
endangered
woody
plant
endemic
to
China,
which
listed
on
the
IUCN
Red
List.
In
natural
state,
viability
of
its
seeds
declines
very
rapidly,
key
status,
but
mechanism
E.
seed
decline
still
unclear.
order
explore
physiological
molecular
seeds,
this
study
used
fresh
as
control
compare
analyze
changes
vitality,
antioxidant
system,
transcription
metabolomics,
when
were
stored
for
1
3
months
at
room
temperature.
The
decreased
continuously
after
month
storage.
activities
superoxide
dismutase
(SOD),
monodehydroascorbate
reductase
(MDHAR),
ascorbate
(AsA),
glutathione
(GSH)
significantly,
while
catalase
(CAT)
activity
increased
gradually
during
viability.
Transcriptomic
results
showed
that
total
801
differentially
expressed
genes
(DEGs)
identified
between
1-month-stored
1,524
3-month-stored
seeds.
Among
them,
expression
CAT
,
MDHAR
GSH
GR
consistent
with
indicators.
Moreover,
WRKY
C3H
bZIP
B3
bHLH
NAC
AP2
/
ERF-ERF
factors
are
important
in
regulating
Metabolomics
types
differential
accumulated
metabolites
(DAMs)
mainly
flavonoids,
amino
acids
derivatives,
phenolic
acids.
combined
analysis
transcriptomics
metabolomics
further
DEGs
DAMs
associated
co-enriched
flavonoid
biosynthesis
tryptophan
metabolism
pathways.
Also
22
genes,
including
ALDH
CHS
C4H
participating
This
also
illustrated
metabolic
pathways
involved
by
acting
system.
These
findings
provide
new
insights
into
.
Язык: Английский
Auxin response factor, RhARF8 contributes to rose flower color fading via regulating anthocyanin biosynthesis by directly activating <i>RhCHSa</i>/<i>c</i> promoter activity
Ornamental Plant Research,
Год журнала:
2025,
Номер
5(1), С. 0 - 0
Опубликована: Янв. 1, 2025
Язык: Английский
Co-overexpression of ZlRc and ZlRd increases flavonoid content, antioxidant activity, and inhibitory effects on against α-glucosidase, α-amylase, pancreatic lipase, and tyrosinase without affecting rice agronomic traits or yield
Plant Physiology and Biochemistry,
Год журнала:
2025,
Номер
unknown, С. 110137 - 110137
Опубликована: Июнь 1, 2025
Язык: Английский
Genome-Wide Characterization and Expression Analysis of CsPALs in Cucumber (Cucumis sativus L.) Reveal Their Potential Roles in Abiotic Stress and Aphid Stress Tolerance
Plants,
Год журнала:
2024,
Номер
13(18), С. 2537 - 2537
Опубликована: Сен. 10, 2024
Phenylalanine
ammonia
lyase
(PAL)
is
a
pivotal
enzyme
in
the
phenylalanine
metabolic
pathway
plants
and
has
crucial
role
plant’s
response
to
environmental
stress.
Although
PAL
family
been
widely
studied
many
plant
species,
limited
known
about
its
particular
cucumbers
under
We
investigated
physicochemical
properties,
gene
structure,
duplication
events,
conserved
motifs,
cis-acting
elements,
protein
interaction
networks,
stress-related
transcriptome
data,
quantitatively
validated
key
genes.
The
main
results
indicated
that
15
genes
were
grouped
into
four
clades:
I,
II,
III
when
arranged
phylogenetic
tree
of
angiosperms.
analysis
promoter
sequence
revealed
presence
multiple
elements
related
hormones
stress
responses
cucumber
(CsPALs).
networks
suggested
CsPAL1
interacts
with
eight
other
members
through
CsELI5
CsHISNA,
directly
proteins
4CL
family.
Further
investigation
expression
patterns
CsPAL
different
tissues
various
treatments
(NaCl,
Cu2+,
Zn2+,
PEG6000,
aphids)
demonstrated
significant
differential
CsPALs
across
tissues.
In
summary,
our
characterization
offers
valuable
insights
provides
important
clues
regarding
molecular
mechanisms
managing
abiotic
biotic
interactions
cucumbers.
Язык: Английский
VdCHS2 Overexpression Enhances Anthocyanin Biosynthesis, Modulates the Composition Ratio, and Increases Antioxidant Activity in Vitis davidii Cells
Antioxidants,
Год журнала:
2024,
Номер
13(12), С. 1472 - 1472
Опубликована: Ноя. 29, 2024
Anthocyanins
are
significant
secondary
metabolites
that
essential
for
plant
growth
and
development,
possessing
properties
such
as
antioxidant,
anti-inflammatory,
anti-cancer
activities
cardiovascular
protection.
They
offer
potential
applications
in
food,
medicine,
cosmetics.
However,
since
anthocyanins
mainly
obtained
through
extraction
chemical
synthesis,
they
encounter
various
challenges,
including
resource
depletion,
ecological
harm,
environmental
pollution,
the
risk
of
toxic
residuals.
To
address
these
issues,
this
study
proposes
a
cell
factory
approach
novel
alternative
solution
anthocyanin
acquisition.
In
study,
VdCHS2
gene
was
successfully
transformed
into
spine
grape
cells,
obtaining
high-yield
line
designated
OE1.
Investigations
light
spectrum
demonstrated
white
promoted
growth,
while
short-wavelength
blue
significantly
boosted
production.
Targeted
metabolomics
analysis
revealed
total
content
OE1
reached
11
mg/g,
representing
60%
increase
compared
to
WT.
A
54
differentially
accumulated
were
identified,
among
which
44
upregulated.
Overexpression
CHS
enhanced
expression
downstream
genes
involved
biosynthesis,
resulting
differential
CHI,
F3Hb,
F3′5′H,
DFR4,
LDOX.
This
led
accumulation
monomers,
predominantly
consisting
3-O-glucosides
3-O-galactosides,
thereby
causing
alterations
levels
composition.
Furthermore,
increased
activity
antioxidant
enzymes,
improved
clearance
reactive
oxygen
species,
reduced
hydrogen
peroxide
(H2O2)
malondialdehyde
(MDA).
The
subsequent
cultivation
line,
conjunction
with
suspension
culture,
established
production,
increasing
yield
shortening
culture
duration.
elucidates
molecular
mechanisms
influenced
compositional
variations.
Additionally,
it
model
small-scale
factory,
providing
theoretical
practical
foundation
targeted
synthesis
components
development
utilization
natural
products.
Язык: Английский