Physiological and Molecular Mechanisms of Lepidopteran Insects: Genomic Insights and Applications of Genome Editing for Future Research DOI Open Access

Dongsheng Niu,

Qing Zhao,

Linbo Xu

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12360 - 12360

Опубликована: Ноя. 18, 2024

Lepidopteran insects are a major threat to global agriculture, causing significant crop losses and economic damage. Traditional pest control methods becoming less effective due the rapid evolution of insecticide resistance. This study explores current status genomic characteristics 1315 records, alongside an overview relevant research, utilizing advanced functional genomics techniques, including RNA-seq CRISPR/Cas9 gene-editing technologies uncover molecular mechanisms underlying Our analysis revealed variability in genome size, assembly quality, chromosome number, which may influence species' biology resistance mechanisms. We identified key resistance-associated genes pathways, detoxification metabolic help these evade chemical control. By employing we directly manipulated confirm their roles resistance, demonstrating potential for targeted interventions management. These findings emphasize value integrating data into development sustainable strategies, reducing reliance on insecticides promoting environmentally friendly integrated management (IPM) approaches. highlights critical role IPM its provide long-term solutions growing challenge

Язык: Английский

Insect Resistance to Insecticides: Causes, Mechanisms, and Exploring Potential Solutions DOI
Juan Boo Liang, Feng Xiao, James Adebayo Ojo

и другие.

Archives of Insect Biochemistry and Physiology, Год журнала: 2025, Номер 118(2)

Опубликована: Фев. 1, 2025

ABSTRACT Insecticides play a crucial role as the primary means of controlling agricultural pests, preventing significant damage to crops. However, misuse these insecticides has led development resistance in insect pests against major classes chemicals. The emergence poses serious threat, especially when alternative options for crop protection are limited farmers. Addressing this challenge and developing new, effective, sustainable pest management approaches is not merely essential but also critically important. In absence solutions, understanding root causes behind insects becomes critical necessity. Without understanding, formulation effective combat remains elusive. With playing vital global food security public health, mitigating paramount. Given growing concern over insecticides, review addresses research gap by thoroughly examining causes, mechanisms, potential solutions. examines factors driving resistance, such evolutionary pressure excessive pesticide use, provides detailed analysis including detoxifying enzyme overproduction target site mutations. Providing an it discusses integrated management, strategic insecticide rotation, use new control technologies biological agents. Emphasizing urgency multifaceted approach, concise roadmap guiding future applications.

Язык: Английский

Процитировано

1

Metabolic resistance to pyrethroids with possible involvement of non-coding ribonucleic acids in Anopheles funestus, the major malaria vector in western Kenya DOI Creative Commons
Isaiah Debrah, Daibin Zhong, Maxwell G. Machani

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 23, 2025

The resurgence of Anopheles funestus, a dominant vector human malaria in western Kenya was partly attributed to insecticide resistance. However, evidence on the molecular basis pyrethroid resistance is limited. Here, we reported metabolic mechanisms and demonstrated that multiple non-coding Ribonucleic Acids (ncRNAs) could play potential role An. funestus Kenya. mosquitoes were sampled using aspiration methods Bungoma, Teso, Siaya, Port Victoria Kombewa F1 progenies exposed deltamethrin (0.05%), permethrin (0.75%), DDT (4%) pirimiphos-methyl (0.25%) following WHO test guidelines. A synergist assay piperonyl butoxide (PBO) conducted determine cytochrome P450s' RNA-seq combined pool specimens resistant unexposed, results compared with those FANG susceptible reference strain. This approach aimed uncover underlying observed phenotypic Pyrethroid all sites an average mortality rate (MR) 57.6%. had highest level (MR = 53%) 11%. Teso lowest 70%) 87%). Resistance only 89%) 85%). full susceptibility P-methyl sites. PBO revealed high (> 98%) pyrethroids except for 96%). Whole transcriptomic analysis showed most gene families associated comprised RNAs (67%), followed by immunity proteins (10%), P450s (6%), cuticular (5%), olfactory (4%), glutathione S-transferases (3%), UDP-glycosyltransferases (2%), ATP-binding cassettes (2%) carboxylesterases (1%). study unveils Kenya, highlighting first time alongside detoxification Targeting intervention development help management.

Язык: Английский

Процитировано

0

Increased expression of an isoform of the long non-coding RNA, lnc37707, is associated with malathion resistance in Bactrocera dorsalis DOI

Li‐Wei Meng,

Zongyang Luo, Fuqiang Zhang

и другие.

Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер 209, С. 106343 - 106343

Опубликована: Фев. 23, 2025

Язык: Английский

Процитировано

0

New Insights into the Regulatory Non-Coding RNAs Mediating Rice–Brown Planthopper Interactions DOI Creative Commons
Liang Hu, Yan Wu,

Wenjun Zha

и другие.

Agronomy, Год журнала: 2025, Номер 15(3), С. 686 - 686

Опубликована: Март 13, 2025

The brown planthopper (Nilaparvata lugens Stål, BPH) is a destructive pest of rice. Non-coding RNAs (ncRNAs) regulate the defense mechanisms in rice and adaptive strategies BPHs. In rice, ncRNAs modulate key resistance pathways such as jasmonic acid biosynthesis, flavonoid production, phenylpropanoid metabolism, which increases BPH resistance. BPHs, processes reproduction, wing polyphenism, facilitate adaptation virulence. Cross-kingdom interactions between BPHs reveal dynamic molecular interplay that underpins this pest–host relationship. These new insights into ncRNA functions will help improve innovative management equip varieties with enhanced

Язык: Английский

Процитировано

0

Physiological and Molecular Mechanisms of Lepidopteran Insects: Genomic Insights and Applications of Genome Editing for Future Research DOI Open Access

Dongsheng Niu,

Qing Zhao,

Linbo Xu

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12360 - 12360

Опубликована: Ноя. 18, 2024

Lepidopteran insects are a major threat to global agriculture, causing significant crop losses and economic damage. Traditional pest control methods becoming less effective due the rapid evolution of insecticide resistance. This study explores current status genomic characteristics 1315 records, alongside an overview relevant research, utilizing advanced functional genomics techniques, including RNA-seq CRISPR/Cas9 gene-editing technologies uncover molecular mechanisms underlying Our analysis revealed variability in genome size, assembly quality, chromosome number, which may influence species' biology resistance mechanisms. We identified key resistance-associated genes pathways, detoxification metabolic help these evade chemical control. By employing we directly manipulated confirm their roles resistance, demonstrating potential for targeted interventions management. These findings emphasize value integrating data into development sustainable strategies, reducing reliance on insecticides promoting environmentally friendly integrated management (IPM) approaches. highlights critical role IPM its provide long-term solutions growing challenge

Язык: Английский

Процитировано

0