Isothermal DNA Amplification with Tailed Primers Coupled with Enzyme-Linked Oligonucleotide Assay for the Sensitive Detection of Trichomonas vaginalis DOI
Christine Aubrey C. Justo, Shaira Jane Acosta, Miriam Jauset‐Rubio

и другие.

ACS Infectious Diseases, Год журнала: 2025, Номер unknown

Опубликована: Июнь 5, 2025

The globally persistent and most common sexually transmitted agent Trichomonas vaginalis continues to be an underestimated public health threat. Infection with T. has been associated reproductive complications increased risk of HIV acquisition. With the available diagnostic tools for trichomoniasis, there is still unmet demand a cost-effective, facile, rapid, robust tool. We report on design utilization tailed primers molecular detection vaginalis, exploiting isothermal DNA amplification solid-phase sandwich colorimetric assay. use primers, in combination enzyme-linked surface-tethered complementary probes, straightforward strategy quantitatively evaluate maximize target-specific by recombinase polymerase (RPA), which does not rely typical nuclease-probe system. quantitative nature microplate-based oligonucleotide assay (ELONA) that relies DNA-DNA hybridization, we systematically optimized RPA conditions maximal specific target fragment genome. developed RPA-ELONA limit 4.03 × 10-4 fg/μL (3.99 aM) synthetic dsDNA more sensitive than PCR-based using same primers. analysis biobanked clinical genomic samples showed 95% accuracy relative qPCR commercial S-DiaMGTV kit. serves as guide further development sandwich-based POCT vaginalis.

Язык: Английский

Vaginal colonization and vertical transmission of Candida species: prevalence and associated factors among pregnant women and their neonates at public health facilities of Northeast Ethiopia DOI Creative Commons
Alemu Gedefie,

Getnet Shimeles,

Hilina Motbainor

и другие.

BMC Pregnancy and Childbirth, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 9, 2025

Vaginal colonization by Candida can lead to vulvovaginal candidiasis, which is the second most prevalent vaginal condition globally. It frequently associated with sepsis and adverse neonatal outcomes in pregnant women. This issue worsening Sub-Saharan Africa, including Ethiopia. However, evidence of existing problem very scarce yet crucial. Thus, this study aimed determine vertical transmission species their factors among women neonates public health facilities northeast A facility-based cross-sectional was conducted at selected Dessie town from April 1 June 30, 2023, 348 newborns, using convenience sampling techniques. Socio-demographic, clinical-related data were collected a pre-tested, semi-structured questionnaire. swab samples pooled swabs external ear, nasal area, umbilical areas newborns transported Amies transport media. The inoculated into Sabouraud Dextrose Agar for isolation, followed inoculation onto standard CHROM agar plate identification, germ-tube test confirmed pseudophyphae C.albicans. Data entered Epi version 4.6.0 software exported analyzed SPSS 25.0. stepwise logistic regression model used identify factors. Variables p < 0.05 95% confidence interval considered statistically significant. total attending delivery included study. maternal rates 14.1% (49/348) 6.3% (22/348), respectively. overall proportion 44.9% (22/49, CI: 41.2, 49.7). Among isolates, 63.3% (31/49) C. albicans 36.7% (18/49) krusei. Gestational diabetes mellitus (AOR: 4.2, 1.23–38.6, P = 0.047) HIV 1.58, 1.11–6.12, 0.049) independently species. Moreover, rural residence (AOR 3.6, 1.37–9.5, 0.010) age above 28 years 2.39, 1.97–5.89, 0.048) findings highlight need effective screening treatment during antenatal care.

Язык: Английский

Процитировано

1
Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis DOI Creative Commons
Christine Aubrey C. Justo, Vasso Skouridou, Piet Cools

и другие.

Sensors & Diagnostics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Aptamer lateral flow assay (ALFA) for testing T. vaginalis (Tv) in cervicovaginal lavage (CVL).

Язык: Английский

Процитировано

1
Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo DOI Creative Commons
Lisa Himschoot, Guy Mulinganya, Tess Rogier

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 14

Опубликована: Янв. 17, 2025

Gardnerella is a key pathogen in bacterial vaginosis (BV), but the role of different species remains unclear. We investigated four species, as well Fannyhessea vaginae, Lactobacillus crispatus and L. iners BV. From 331 pregnant women from Democratic Republic Congo, BV was diagnosed using Nugent scoring cervicovaginal lavage used to quantify G. leopoldii, piotii, swidsinskii, vaginalis, F. by qPCR. Univariate associations between these clinical outcomes were assessed. A logistic regression model ROC curves calculated determine best diagnostic marker for Here, (75.8%) most prevalent vaginalis (36.0%) common species. All spp. (50.9-57.9%) with (asymptomatic) analysis revealed no significant symptoms BV, while vaginae (positive Whiff test, high pH), (high pH) (low associated signs piotii markers urinary tract infection. Women had higher odds delivering preterm. analyses showed that (AUC 0.81), combined further increased performance 0.90). involved although none important emerged superior molecular

Язык: Английский

Процитировано

0
Isothermal DNA Amplification with Tailed Primers Coupled with Enzyme-Linked Oligonucleotide Assay for the Sensitive Detection of Trichomonas vaginalis DOI
Christine Aubrey C. Justo, Shaira Jane Acosta, Miriam Jauset‐Rubio

и другие.

ACS Infectious Diseases, Год журнала: 2025, Номер unknown

Опубликована: Июнь 5, 2025

The globally persistent and most common sexually transmitted agent Trichomonas vaginalis continues to be an underestimated public health threat. Infection with T. has been associated reproductive complications increased risk of HIV acquisition. With the available diagnostic tools for trichomoniasis, there is still unmet demand a cost-effective, facile, rapid, robust tool. We report on design utilization tailed primers molecular detection vaginalis, exploiting isothermal DNA amplification solid-phase sandwich colorimetric assay. use primers, in combination enzyme-linked surface-tethered complementary probes, straightforward strategy quantitatively evaluate maximize target-specific by recombinase polymerase (RPA), which does not rely typical nuclease-probe system. quantitative nature microplate-based oligonucleotide assay (ELONA) that relies DNA-DNA hybridization, we systematically optimized RPA conditions maximal specific target fragment genome. developed RPA-ELONA limit 4.03 × 10-4 fg/μL (3.99 aM) synthetic dsDNA more sensitive than PCR-based using same primers. analysis biobanked clinical genomic samples showed 95% accuracy relative qPCR commercial S-DiaMGTV kit. serves as guide further development sandwich-based POCT vaginalis.

Язык: Английский

Процитировано

0