Vaginal colonization and vertical transmission of Candida species: prevalence and associated factors among pregnant women and their neonates at public health facilities of Northeast Ethiopia
BMC Pregnancy and Childbirth,
Год журнала:
2025,
Номер
25(1)
Опубликована: Янв. 9, 2025
Vaginal
colonization
by
Candida
can
lead
to
vulvovaginal
candidiasis,
which
is
the
second
most
prevalent
vaginal
condition
globally.
It
frequently
associated
with
sepsis
and
adverse
neonatal
outcomes
in
pregnant
women.
This
issue
worsening
Sub-Saharan
Africa,
including
Ethiopia.
However,
evidence
of
existing
problem
very
scarce
yet
crucial.
Thus,
this
study
aimed
determine
vertical
transmission
species
their
factors
among
women
neonates
public
health
facilities
northeast
A
facility-based
cross-sectional
was
conducted
at
selected
Dessie
town
from
April
1
June
30,
2023,
348
newborns,
using
convenience
sampling
techniques.
Socio-demographic,
clinical-related
data
were
collected
a
pre-tested,
semi-structured
questionnaire.
swab
samples
pooled
swabs
external
ear,
nasal
area,
umbilical
areas
newborns
transported
Amies
transport
media.
The
inoculated
into
Sabouraud
Dextrose
Agar
for
isolation,
followed
inoculation
onto
standard
CHROM
agar
plate
identification,
germ-tube
test
confirmed
pseudophyphae
C.albicans.
Data
entered
Epi
version
4.6.0
software
exported
analyzed
SPSS
25.0.
stepwise
logistic
regression
model
used
identify
factors.
Variables
p
<
0.05
95%
confidence
interval
considered
statistically
significant.
total
attending
delivery
included
study.
maternal
rates
14.1%
(49/348)
6.3%
(22/348),
respectively.
overall
proportion
44.9%
(22/49,
CI:
41.2,
49.7).
Among
isolates,
63.3%
(31/49)
C.
albicans
36.7%
(18/49)
krusei.
Gestational
diabetes
mellitus
(AOR:
4.2,
1.23–38.6,
P
=
0.047)
HIV
1.58,
1.11–6.12,
0.049)
independently
species.
Moreover,
rural
residence
(AOR
3.6,
1.37–9.5,
0.010)
age
above
28
years
2.39,
1.97–5.89,
0.048)
findings
highlight
need
effective
screening
treatment
during
antenatal
care.
Язык: Английский
Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of Trichomonas vaginalis
Sensors & Diagnostics,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Aptamer
lateral
flow
assay
(ALFA)
for
testing
T.
vaginalis
(Tv)
in
cervicovaginal
lavage
(CVL).
Язык: Английский
Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2025,
Номер
14
Опубликована: Янв. 17, 2025
Gardnerella
is
a
key
pathogen
in
bacterial
vaginosis
(BV),
but
the
role
of
different
species
remains
unclear.
We
investigated
four
species,
as
well
Fannyhessea
vaginae,
Lactobacillus
crispatus
and
L.
iners
BV.
From
331
pregnant
women
from
Democratic
Republic
Congo,
BV
was
diagnosed
using
Nugent
scoring
cervicovaginal
lavage
used
to
quantify
G.
leopoldii,
piotii,
swidsinskii,
vaginalis,
F.
by
qPCR.
Univariate
associations
between
these
clinical
outcomes
were
assessed.
A
logistic
regression
model
ROC
curves
calculated
determine
best
diagnostic
marker
for
Here,
(75.8%)
most
prevalent
vaginalis
(36.0%)
common
species.
All
spp.
(50.9-57.9%)
with
(asymptomatic)
analysis
revealed
no
significant
symptoms
BV,
while
vaginae
(positive
Whiff
test,
high
pH),
(high
pH)
(low
associated
signs
piotii
markers
urinary
tract
infection.
Women
had
higher
odds
delivering
preterm.
analyses
showed
that
(AUC
0.81),
combined
further
increased
performance
0.90).
involved
although
none
important
emerged
superior
molecular
Язык: Английский
Isothermal DNA Amplification with Tailed Primers Coupled with Enzyme-Linked Oligonucleotide Assay for the Sensitive Detection of Trichomonas vaginalis
ACS Infectious Diseases,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 5, 2025
The
globally
persistent
and
most
common
sexually
transmitted
agent
Trichomonas
vaginalis
continues
to
be
an
underestimated
public
health
threat.
Infection
with
T.
has
been
associated
reproductive
complications
increased
risk
of
HIV
acquisition.
With
the
available
diagnostic
tools
for
trichomoniasis,
there
is
still
unmet
demand
a
cost-effective,
facile,
rapid,
robust
tool.
We
report
on
design
utilization
tailed
primers
molecular
detection
vaginalis,
exploiting
isothermal
DNA
amplification
solid-phase
sandwich
colorimetric
assay.
use
primers,
in
combination
enzyme-linked
surface-tethered
complementary
probes,
straightforward
strategy
quantitatively
evaluate
maximize
target-specific
by
recombinase
polymerase
(RPA),
which
does
not
rely
typical
nuclease-probe
system.
quantitative
nature
microplate-based
oligonucleotide
assay
(ELONA)
that
relies
DNA-DNA
hybridization,
we
systematically
optimized
RPA
conditions
maximal
specific
target
fragment
genome.
developed
RPA-ELONA
limit
4.03
×
10-4
fg/μL
(3.99
aM)
synthetic
dsDNA
more
sensitive
than
PCR-based
using
same
primers.
analysis
biobanked
clinical
genomic
samples
showed
95%
accuracy
relative
qPCR
commercial
S-DiaMGTV
kit.
serves
as
guide
further
development
sandwich-based
POCT
vaginalis.
Язык: Английский