Single nucleotide polymorphisms in the Dicer gene modify the risk of systemic lupus erythematosus

European Journal of Inflammation, Год журнала: 2024, Номер 22

Опубликована: Янв. 1, 2024

Objective MicroRNA-related single-nucleotide polymorphisms (miR-SNPs) can alter microRNA (miRNA) expression profiles, thereby influencing the risk of rheumatic diseases. Herrin a case control study, six miR-SNPs in miRNA processing machinery genes, namely RAN (rs14035), XPO5 (rs11077), Dicer (rs3742330), GEMIN3 (rs197412), GEMIN4 (rs2740348), and TNRC6B (rs9623117), were genotyped to assess their correlation with systemic lupus erythematosus (SLE). Methods We included 119 patients SLE 130 healthy controls. The genotypes determined using polymerase chain reaction (PCR). Serum cytokine levels assessed cytometric bead array, fluorescent probe technology was used determine plasma reactive oxygen species (ROS) levels. Results AA genotype correlated 0.566-fold decreased compared that AG + GG (odds ratio, 0.566; 95% CI, 0.342–0.935; p = .026), rs3742330 A allele associated significantly ( .035) rs3742330G allele. Additionally, carriers exhibited lower interleukin-6 (IL-6) blood .013). Subsequent analysis revealed increased ROS production than controls (621.042 ± 425.285 vs 499.966 302.273, .011). Conclusion Our findings suggest generation participates pathogenesis. identification gene SNP as potential modifier via mediating IL-6 overproduction suggests avenue for targeted interventions manage its immune dysregulation.

Язык: Английский

---

The Functional Mechanism of BP9 in Promoting B Cell Differentiation and Inducing Antigen Presentation

Vaccines, Год журнала: 2024, Номер 12(6), С. 607 - 607

Опубликована: Июнь 1, 2024

The Bursa of Fabricius, an avian unique humoral immune organ, is instrumental to B cell development. Bursal-derived peptide BP9 fosters B-cell development and formation. Yet, the exact mechanism wherein impacts differentiation antigenic presentation remains undefined. In this paper, activation in spleen cells from mice immunized with AIV vaccine were detected following flow cytometry (FCM) analysis. Furthermore, molecular vivo was investigated RNA sequencing technology. To verify potential functional process, transcriptome basis chicken macrophages stimulated by measured via high-throughput results proved that when given experimental dosages, notably accelerated total cells, enhanced plasma production. gene expression profiles 0.01 mg/mL disclosed initiated enrichment several biological functions significantly key pathways mice. Crucially, a 4093 differentially expressed genes identified stimulation, including 943 upregulated 3150 downregulated genes. Additionally, induced various cytokine productions macrophage HD11 activated 9 20 differential miRNAs, which involved signal processes. multiple transcription factors related antigen summary, these suggested might promote induce presentation, provide valuable insights into upon bursal-derived immunomodulating stimulation solid groundwork for enhancing vaccine-induced immunity.

Язык: Английский

---

Molecular profiling and therapeutic tailoring to address disease heterogeneity in systemic lupus erythematosus

Clinical and Experimental Medicine, Год журнала: 2024, Номер 24(1)

Опубликована: Сен. 19, 2024

Язык: Английский

---

The Utility of RNA Triplex Formation in Autoimmune Disease: Identification of Therapeutic Dual Synergistic MicroRNAs in Systemic Lupus Erythematosus—A Bioinformatics Approach

IFMBE proceedings, Год журнала: 2024, Номер unknown, С. 493 - 512

Опубликована: Янв. 1, 2024

Язык: Английский

---

The new era of immune skin diseases: Exploring advances in basic research and clinical translations

Journal of Translational Autoimmunity, Год журнала: 2024, Номер 8, С. 100232 - 100232

Опубликована: Янв. 13, 2024

Язык: Английский

---