Efficacy of the immediate adipose-derived stromal vascular fraction autograft on functional sensorimotor recovery after spinal cord contusion in rats DOI Creative Commons

Céline Ertlen,

Mostafa Seblani, M Bonnet

и другие.

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 2, 2024

Abstract Background Spinal cord injuries (SCI) lead to functional alteration with important consequences such as motor and sensory disorders. The repair strategies developed date remain ineffective. adipose tissue-derived stromal vascular fraction (SVF) is composed of a cocktail cells trophic, pro-angiogenic immunomodulatory effects. Numerous therapeutic benefits were shown for tissue reconstitution, peripheral neuropathy the improvement neurodegenerative diseases. Here, efficacy SVF on sensorimotor recovery after an acute thoracic spinal contusion in adult rats was determined. Method Male Sprague Dawley ( n = 45) divided into 3 groups: SHAM (without SCI treatment), NaCl (animals lesion receiving saline injection through dura mater) fat removed from adipocytes mater). Some animals sacrificed 14 days start experiment determine inflammatory reaction by measuring interleukin-1β, interleukin-6 Tumor Necrosis Factor-α area. Other followed once week 12 weeks assess (postural locomotor activities, coordination). At end this period, reflexivity (rate-dependent depression H-reflex) physiological adjustments (ventilatory response metabosensitive muscle activation following fatigue) measured electrophysiological tools. Results Compared non-treated animals, results indicated that reduced endogenous inflammation increased behavioral treated animals. Moreover, H-reflex ventilatory fatigue found be comparable between groups. Conclusion Our highlight effectiveness its high potential improve functions restore segmental loop communication supra- sub-lesional regions traumatic contusion. Graphical

Язык: Английский

NEMO‐Binding Domain/IKKγ Inhibitory Peptide Alleviates Neuronal Pyroptosis in Spinal Cord Injury by Inhibiting ASMase‐Induced Lysosome Membrane Permeabilization DOI Creative Commons

Yibo Geng,

Junsheng Lou, Junnan Wu

и другие.

Advanced Science, Год журнала: 2024, Номер 11(40)

Опубликована: Сен. 3, 2024

Abstract A short peptide termed NEMO‐binding domain (NBD) has an inhibitory effect on nuclear factor kappa‐B (NF‐κB). Despite its efficacy in inhibiting inflammatory responses, the precise neuroprotective mechanisms of NBD spinal cord injury (SCI) remain unclear. This study aims to determine whether pyroptosis‐related aspects involved effects post‐SCI.Using RNA sequencing, molecular SCI are explored. The evaluation functional recovery is performed using Basso mouse scale, Nissl staining, footprint analysis, Masson's trichrome and HE staining. Western blotting, enzyme‐linked immunosorbent assays, immunofluorescence assays used examine pyroptosis, autophagy, lysosomal membrane permeabilization (LMP), acid sphingomyelinase (ASMase), NF‐κB/p38‐MAPK related signaling pathway.NBD mitigated glial scar formation, reduced motor neuron death, enhanced mice. Additionally, inhibits ameliorate LMP‐induced autophagy flux disorder post‐SCI. Mechanistically, alleviates LMP subsequently enhances by ASMase through NF‐κB/p38‐MAPK/Elk‐1/Egr‐1 cascade, thereby mitigating neuronal death. contributes restoration suppressing ASMase‐mediated depression, pyroptosis following SCI, which may have potential clinical application value.

Язык: Английский

Процитировано

7
Mode of action of astrocytes in pain: From the spinal cord to the brain DOI

Yong-long Chen,

Xiang-lan Feng,

Chi‐Wai Cheung

и другие.

Progress in Neurobiology, Год журнала: 2022, Номер 219, С. 102365 - 102365

Опубликована: Окт. 10, 2022

Язык: Английский

Процитировано

29
Molecular Mechanisms and Clinical Application of Multipotent Stem Cells for Spinal Cord Injury DOI Creative Commons
Michał Szymoniuk, Jakub Litak, Leon Sakwa

и другие.

Cells, Год журнала: 2022, Номер 12(1), С. 120 - 120

Опубликована: Дек. 28, 2022

Spinal Cord Injury (SCI) is a common neurological disorder with devastating psychical and psychosocial sequelae. The majority of patients after SCI suffer from permanent disability caused by motor dysfunction, impaired sensation, neuropathic pain, spasticity as well urinary complications, small number experience complete recovery. Current standard treatment modalities the aim to prevent secondary injury provide limited recovery lost functions. Stem Cell Therapy (SCT) represents an emerging approach using differentiation, paracrine, self-renewal capabilities stem cells regenerate injured spinal cord. To date, multipotent including mesenchymal (MSCs), neural (NSCs), hematopoietic (HSCs) represent most investigated types for in preclinical clinical studies. microenvironment has significant impact on survival, proliferation, differentiation transplanted cells. Therefore, deep understanding pathophysiology molecular mechanisms through which act may help improve efficacy SCT find new therapeutic approaches such stem-cell-derived exosomes, gene-modified cells, scaffolds, nanomaterials. In this literature review, pathogenesis action MSCs, NSCs, HSCs are comprehensively described. Moreover, treatment, optimal protocol cell administration, recent based or combined also discussed.

Язык: Английский

Процитировано

27
Aucubin promoted neuron functional recovery by suppressing inflammation and neuronal apoptosis in a spinal cord injury model DOI
Shining Xiao,

Nanshan Zhong,

Quanming Yang

и другие.

International Immunopharmacology, Год журнала: 2022, Номер 111, С. 109163 - 109163

Опубликована: Авг. 19, 2022

Язык: Английский

Процитировано

21
The Inflammatory Response after Moderate Contusion Spinal Cord Injury: A Time Study DOI Creative Commons
Minna Christiansen Lund,

Ditte Gry Ellman,

Maiken Nissen

и другие.

Biology, Год журнала: 2022, Номер 11(6), С. 939 - 939

Опубликована: Июнь 20, 2022

Spinal cord injury (SCI) initiates detrimental cellular and molecular events that lead to acute delayed neuroinflammation. Understanding the role of inflammatory response in SCI requires insight into temporal synthesis mediators. We subjected C57BL/6J mice investigated reactions. examined activation, recruitment, polarization microglia infiltrating immune cells, focusing specifically on tumor necrosis factor (TNF) its receptors TNFR1 TNFR2. In phase, TNF expression increased glial cells neuron-like followed by cells. TNFR2 levels phase were found preferentially neurons respectively. The was dominated infiltration granulocytes macrophages. Microglial/macrophage Arg1 from 1-7 days after SCI, an increase Itgam, Cx3cr1, P2ry12, which remained elevated throughout study. By 21 28 lesion core populated galectin-3+, CD68+, CD11b+ microglia/macrophages, surrounded a scar consisting GFAP+ astrocytes. Findings verified postmortem tissue individuals with SCI. Our findings support consensus future neuroprotective immunotherapies should aim selectively neutralize signaling while sustaining pro-regenerative processes.

Язык: Английский

Процитировано

20
Small extracellular vesicles derived from four dimensional-culture of mesenchymal stem cells induce alternatively activated macrophages by upregulating IGFBP2/EGFR to attenuate inflammation in the spinal cord injury of rats DOI Creative Commons
Junhua Wang,

Qing-shuai Wei,

Yue Yang

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11

Опубликована: Апрель 28, 2023

Effectively reducing the inflammatory response after spinal cord injury (SCI) is a challenging clinical problem and subject of active investigation. This study employed porous scaffold-based three dimensional long-term culture technique to obtain human umbilical mesenchymal stem cell (hUC-MSC)-derived Small Extracellular Vesicles (sEVs) (three over time, "4D-sEVs"). Moreover, vesicle size, number, inner protein concentrations MSC 4D-sEVs contained altered profiles compared with those derived from 2D conditions. A proteomics analysis suggested broad changes, especially significant upregulation Epidermal Growth Factors Receptor (EGFR) Insulin-like Factor Binding Protein 2 (IGFBP2) in 2D-sEVs. The endocytosis allowed for binding EGFR IGFBP2, leading downstream STAT3 phosphorylation IL-10 secretion effective induction macrophages/microglia polarization pro-inflammatory M1 anti-inflammatory M2 phenotype, both vitro injured areas rats compressive/contusive SCI. reduction neuroinflammation delivery site epicenter led neuroprotection, as evidenced by number surviving neurons. Therefore, applying this novel 4D culture-derived could effectively curb increase tissue repair

Язык: Английский

Процитировано

12
Neuroprotective effects of tetramethylpyrazine on spinal cord injury-Related neuroinflammation mediated by P2X7R/NLRP3 interaction DOI
Xiao Fan,

Chunyan Zang,

Kecheng Lao

и другие.

European Journal of Pharmacology, Год журнала: 2023, Номер 964, С. 176267 - 176267

Опубликована: Дек. 9, 2023

Язык: Английский

Процитировано

12
The acute spinal cord injury microenvironment and its impact on the homing of mesenchymal stem cells DOI
Jinyi Liu,

Longju Qi,

Shengzhe Bao

и другие.

Experimental Neurology, Год журнала: 2024, Номер 373, С. 114682 - 114682

Опубликована: Янв. 9, 2024

Язык: Английский

Процитировано

5
The phenotypic changes of Schwann cells promote the functional repair of nerve injury DOI
Shu‐Fen Liao, Yan Chen, Ying Luo

и другие.

Neuropeptides, Год журнала: 2024, Номер 106, С. 102438 - 102438

Опубликована: Май 11, 2024

Язык: Английский

Процитировано

5
Multiple strategies enhance the efficacy of MSCs transplantation for spinal cord injury DOI Open Access

Qi-Ming Pang,

Kua-Qian Deng,

Meng Zhang

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2022, Номер 157, С. 114011 - 114011

Опубликована: Ноя. 18, 2022

Spinal cord injury (SCI) is a serious complication of the central nervous system (CNS) after spine injury, often resulting in severe sensory, motor, and autonomic dysfunction below level injury. To date, there no effective treatment strategy for SCI. Recently, stem cell therapy has brought hope to patients with neurological diseases. Mesenchymal cells (MSCs) are considered be most promising source cellular SCI due their immunomodulatory, neuroprotective angiogenic potential. Considering limited therapeutic effect MSCs complex pathophysiological environment following SCI, this paper not only reviews specific mechanism facilitate repair, but also further discusses research status these pluripotent combined other approaches promote anatomical functional recovery post-SCI.

Язык: Английский

Процитировано

19