Current Issues in Molecular Biology,
Год журнала:
2023,
Номер
45(7), С. 5830 - 5848
Опубликована: Июль 13, 2023
Mechanotransduction
(MT)
is
inseparable
from
the
pathobiology
of
heart
failure
(HF).
However,
effects
mechanical
forces
on
HF
remain
unclear.
This
review
briefly
describes
how
Piezo1
functions
in
HF-affected
cells,
including
endothelial
cells
(ECs),
cardiac
fibroblasts
(CFs),
cardiomyocytes
(CMs),
and
immune
cells.
a
mechanosensitive
ion
channel
that
has
been
extensively
studied
recent
years.
responds
to
different
converts
them
into
intracellular
signals.
The
pathways
modulate
switch
have
also
described.
Experimental
drugs
specifically
activate
Piezo1-like
proteins,
such
as
Yoda1,
Jedi1,
Jedi2,
are
available
for
clinical
studies
treat
Piezo1-related
diseases.
only
ion-channel-specific
inhibitor
GsMTx4,
which
can
turn
off
by
modulating
local
membrane
tension.
Ultrasound
waves
switching
vitro
with
assistance
microbubbles.
provides
new
possible
targets
therapy
exploring
cellular
involved
progression
disease.
Modulation
activity
may,
therefore,
effectively
delay
failure.
Biomedicines,
Год журнала:
2023,
Номер
11(7), С. 1942 - 1942
Опубликована: Июль 8, 2023
Biomechanical
studies
are
expanding
across
a
variety
of
fields,
from
biomedicine
to
biomedical
engineering.
From
the
molecular
system
level,
mechanical
stimuli
crucial
regulators
development
organs
and
tissues,
their
growth
related
processes
such
as
remodelling,
regeneration
or
disease.
When
dealing
with
cell
mechanics,
various
experimental
techniques
have
been
developed
analyse
passive
response
cells;
however,
variability
extraction
process,
complex
procedures
different
models
assumptions
may
affect
resulting
properties.
For
these
purposes,
this
review
was
aimed
at
collecting
available
literature
focused
on
chondrocyte
chondron
biomechanics
direct
connection
biochemical
functions
activities,
in
order
point
out
important
information
regarding
planning
an
test
comparison
results.
In
particular,
highlighted
(i)
most
common
used,
(ii)
results
adopted
by
authors,
(iii)
critical
perspective
features
that
could
finally
(iv)
quantification
structural
changes
due
degenerative
pathology
osteoarthritis.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(4), С. 4505 - 4518
Опубликована: Янв. 19, 2024
The
NLRP3
inflammasome
is
a
crucial
component
of
the
innate
immune
system,
playing
pivotal
role
in
initiating
and
regulating
body's
inflammatory
response
to
various
pathogens
cellular
damage.
Environmental
stimuli,
such
as
temperature,
pH
level,
nutrient
availability,
can
influence
behavior
functions
cells,
including
cell
activity,
proliferation,
cytokine
production.
However,
there
limited
understanding
regarding
how
mechanical
forces,
like
shear
stress,
govern
intrinsic
reaction,
particularly
activation
inflammasome,
stress
impacts
through
its
capacity
induce
alterations
gene
expression
secretion.
Here,
we
investigated
act
priming
signal
by
exposing
immortalized
bone
marrow-derived
macrophages
(iBMDMs)
numerous
physiologically
relevant
magnitudes
before
chemically
inducing
activation.
We
demonstrated
that
large
was
able
prime
iBMDMs
more
effectively
for
compared
lower
magnitudes,
quantified
percentage
cells
where
ASC-CFP
specks
formed
IL-1β
secretion,
hallmarks
Testing
this
caspase-1
knockout
showed
primarily
primed
due
exposure.
Quantitative
polymerase
chain
reaction
(qPCR)
small-molecule
inhibitor
study
mechanistically
determined
regulates
upregulating
Piezo1,
IKKβ,
NLRP3.
These
findings
offer
insights
into
mechanistic
relationship
among
physiological
stresses,
activation,
their
impact
on
progression
diseases
interconnected
pathogenesis.
Frontiers in Cell and Developmental Biology,
Год журнала:
2022,
Номер
10
Опубликована: Авг. 19, 2022
Biophysical
and
biochemical
cues
work
in
concert
to
regulate
angiogenesis.
These
guide
angiogenesis
during
development
wound
healing.
Abnormal
contribute
pathological
tumor
progression.
In
this
review,
we
summarize
the
known
signaling
pathways
involved
mechanotransduction
important
We
discuss
how
variation
mechanical
microenvironment,
terms
of
stiffness,
ligand
availability,
topography,
can
modulate
process.
also
present
an
integrated
view
on
perturbations,
such
as
stretching
fluid
shearing,
alter
angiogenesis-related
signal
transduction
acutely,
leading
downstream
gene
expression.
Tissue
engineering-based
approaches
study
are
reviewed
too.
Future
directions
aid
efforts
unveiling
comprehensive
picture
proposed.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(7), С. 6513 - 6513
Опубликована: Март 30, 2023
Since
the
recent
discovery
of
mechanosensitive
Piezo1
channels,
many
studies
have
addressed
role
channel
in
various
physiological
or
even
pathological
processes
different
organs.
Although
number
on
their
effects
musculoskeletal
system
is
constantly
increasing,
we
are
still
far
from
a
precise
understanding.
In
this
review,
knowledge
available
so
regarding
summarized,
reviewing
results
achieved
field
skeletal
muscles,
bones,
joints
and
cartilage,
tendons
ligaments,
as
well
intervertebral
discs.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2023,
Номер
11
Опубликована: Ноя. 20, 2023
Mechanical
stimuli
regulate
the
chondrogenic
differentiation
of
mesenchymal
stem
cells
and
homeostasis
chondrocytes,
thus
affecting
implant
success
in
cartilage
tissue
engineering.
The
mechanical
microenvironment
plays
fundamental
roles
maturation
maintenance
natural
articular
cartilage,
progression
osteoarthritis
Hence,
engineering
attempts
to
mimic
this
environment
vivo
obtain
implants
that
enable
a
superior
regeneration
process.
However,
specific
type
loading,
its
optimal
regime,
underlying
molecular
mechanisms
are
still
under
investigation.
First,
review
delineates
composition
structure
indicating
morphology
chondrocytes
components
extracellular
matrix
differ
from
each
other
resist
forces
three
top-to-bottom
overlapping
zones.
Moreover,
results
research
experiments
clinical
trials
focusing
on
effect
compression,
fluid
shear
stress,
hydrostatic
pressure,
osmotic
pressure
presented
critically
evaluated.
As
key
direction,
latest
advances
involved
transduction
external
signals
into
biological
discussed.
These
sensed
by
receptors
cell
membrane,
such
as
primary
cilia,
integrins,
ion
channels,
which
next
activate
downstream
pathways.
Finally,
biomaterials
with
various
modifications
properties
self-designed
bioreactors
for
experiment
vitro
outlined.
An
improved
understanding
biomechanically
driven
is
expected
lead
efficient
repair
degeneration
disease.
