Low levels of neutralizing antibodies against XBB Omicron subvariants after BA.5 infection

Signal Transduction and Targeted Therapy, Год журнала: 2023, Номер 8(1)

Опубликована: Июнь 19, 2023

Abstract The COVID-19 response strategies in Chinese mainland were recently adjusted due to the reduced pathogenicity and enhanced infectivity of Omicron subvariants. In Chengdu, China, an infection wave was predominantly induced by BA.5 subvariant. It is crucial determine whether hybrid anti-SARS-CoV-2 immunity following infection, coupled with a variety immune background, sufficient shape responses against newly emerged subvariants, especially for XBB lineages. To investigate this, we collected serum nasal swab samples from 108 participants who had been infected this wave, evaluated neutralization pseudoviruses. Our results showed that convalescent sera individuals, regardless vaccination history, remarkably compromised capacities XBB.1.5 Although post-vaccination breakthrough slightly elevated plasma neutralizing antibodies part pseudoviruses, activities impaired Furthermore, analyzed impacts number vaccinations, age, sex on humoral cellular after infection. findings suggest lineages elicited current are remained at low levels, indicating urgent need development next-generation vaccines designed based sub-lineages other future variants.

Язык: Английский

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B-cell and antibody responses to SARS-CoV-2: infection, vaccination, and hybrid immunity

Cellular and Molecular Immunology, Год журнала: 2023, Номер 21(2), С. 144 - 158

Опубликована: Ноя. 10, 2023

Abstract The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019 prompted scientific, medical, and biotech communities to investigate infection- vaccine-induced immune responses the context this pathogen. B-cell antibody are at center these investigations, as neutralizing antibodies (nAbs) an important correlate protection (COP) from infection primary target SARS-CoV-2 vaccine modalities. In addition absolute levels, nAb longevity, neutralization breadth, immunoglobulin isotype subtype composition, presence mucosal sites have become topics for scientists health policy makers. recent pandemic was still is a unique setting which study de novo memory (MBC) dynamic interplay immunity. It also provided opportunity explore new platforms, such mRNA or adenoviral vector vaccines, unprecedented cohort sizes. Combined with technological advances years, situation has detailed mechanistic insights into development but revealed some unexpected findings. review, we summarize key findings last 2.5 years regarding immunity, believe significant value not only future vaccination approaches endemic settings.

Язык: Английский

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Bivalent COVID-19 mRNA booster vaccination (BA.1 or BA.4/BA.5) increases neutralization of matched Omicron variants

npj Vaccines, Год журнала: 2023, Номер 8(1)

Опубликована: Авг. 4, 2023

Abstract We report SARS-CoV-2 neutralizing antibody titers in sera of triple-vaccinated individuals who received a booster dose an original monovalent or bivalent BA.1- BA.4/BA.5-adapted vaccine had breakthrough infection with Omicron variants BA.1, BA.2 BA.4/BA.5. A BA.4/BA.5 Omicron-breakthrough induced increased Omicron-neutralization compared the booster. The XBB.1.5 variant effectively evaded neutralizing-antibody responses elicited by current vaccines and/or previous variants.

Язык: Английский

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Imprinted antibody responses against SARS-CoV-2 Omicron sublineages

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Май 10, 2022

SARS-CoV-2 Omicron sublineages carry distinct spike mutations and represent an antigenic shift resulting in escape from antibodies induced by previous infection or vaccination. We show that hybrid immunity vaccine boosters result potent plasma neutralizing activity against BA.1 BA.2 breakthrough infections, but not vaccination-only, induce the nasal mucosa. Consistent with immunological imprinting, most derived memory B cells of cases cross-react Wuhan-Hu-1, receptor-binding domains whereas primary infections elicit narrow specificity. While clinical have reduced neutralization Omicron, we identified ultrapotent pan-variant antibody, is unaffected any lineage a strong candidate for development.

Язык: Английский

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Reduced Sensitivity of Commercial Spike-Specific Antibody Assays after Primary Infection with the SARS-CoV-2 Omicron Variant

Microbiology Spectrum, Год журнала: 2022, Номер 10(5)

Опубликована: Авг. 25, 2022

The SARS-CoV-2 Omicron variant is characterized by substantial changes in the antigenic structure of Spike (S) protein. Therefore, antibodies induced primary infection lack neutralizing activity against earlier variants. In this study, we analyzed whether these impact sensitivity commercial anti-SARS-CoV-2 antibody assays. Sera from 37 unvaccinated, convalescent individuals after putative were tested with a panel 20 immunoassays. As controls, used samples 43 ancestral wild-type strain. addition, variant-specific live-virus neutralization assays as reference for presence SARS-CoV-2-specific samples. Notably, convalescents, there was statistically significant reduction all containing S or its receptor-binding-domain (RBD) antigens. Furthermore, levels quantified displayed weaker correlation Omicron-specific titers than those wild type. contrast, nucleocapsid-protein-specific immunoassays similar and Omicron-infected subjects. summary, lead to reduced immunoreactivity current S- RBD-specific assays, impairing their diagnostic performance.

Язык: Английский

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Importance, Applications and Features of Assays Measuring SARS-CoV-2 Neutralizing Antibodies

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(6), С. 5352 - 5352

Опубликована: Март 10, 2023

More than three years ago, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) caused unforeseen COVID-19 pandemic with millions of deaths. In meantime, SARS-CoV-2 has become endemic and is now part repertoire viruses causing seasonal severe respiratory infections. Due to several factors, among them development immunity through natural infection, vaccination current dominance seemingly less pathogenic strains belonging omicron lineage, situation stabilized. However, challenges remain possible new occurrence highly variants remains a threat. Here we review development, features importance assays measuring neutralizing antibodies (NAbs). particular focus on in vitro infection molecular interaction studying binding receptor domain (RBD) its cognate cellular ACE2. These assays, but not measurement SARS-CoV-2-specific per se, can inform us whether produced by convalescent or vaccinated subjects may protect against thus have potential predict risk becoming newly infected. This information extremely important given fact that considerable number subjects, vulnerable persons, respond poorly production antibodies. Furthermore, these allow determine evaluate virus-neutralizing capacity induced vaccines administration plasma-, immunoglobulin preparations, monoclonal antibodies, ACE2 synthetic compounds be used for therapy assist preclinical evaluation vaccines. Both types relatively quickly adapted emerging virus about magnitude cross-neutralization, which even estimate infected appearing variants. Given paramount discuss their specific features, advantages disadvantages, technical aspects yet fully resolved issues, such as cut-off levels predicting degree vivo protection.

Язык: Английский

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Immune interference in effectiveness of influenza and COVID-19 vaccination

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Апрель 19, 2023

Vaccines are known to function as the most effective interventional therapeutics for controlling infectious diseases, including polio, smallpox, rabies, tuberculosis, influenza and SARS-CoV-2. Smallpox has been eliminated completely polio is almost extinct because of vaccines. Rabies vaccines Bacille Calmette-Guérin (BCG) could effectively protect humans against respective infections. However, both COVID-19 unable eliminate these two diseases their highly variable antigenic sites in viral proteins. Vaccine effectiveness (VE) be negatively influenced (i.e., interfered with) by immune imprinting previous infections or vaccinations, repeated vaccinations interfere with VE due mismatch between vaccine strains endemic strains. Moreover, also when more than one kind administrated concomitantly co-administrated), suggesting that modulated vaccine-induced immunity. In this review, we revisit evidence support result from vaccine, interference co-administration types discussed. Regarding development next-generation vaccines, researchers should focus on induction cross-reactive T-cell responses naive B-cell overcome negative effects system itself. The strategy co-administrating needs considered carefully clinical data needed verify safe immunogenic.

Язык: Английский

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Neutralization of SARS‐CoV‐2 Omicron XBB.1.5 and JN.1 variants after COVID‐19 booster‐vaccination and infection

Journal of Medical Virology, Год журнала: 2024, Номер 96(7)

Опубликована: Июль 1, 2024

Abstract SARS‐CoV‐2 Omicron lineages continue to emerge and evolve into new sublineages, causing infection waves throughout 2022 2023, which has been attributed immune escape. We examined neutralizing antibody responses the recently emerged JN.1 variant in comparison ancestral D614G BA.1, BA.2, BA.5, XBB.1.5 variants. tested 79 human sera from cohorts with different combinations of vaccinations infections, including 23 individuals who had repeatedly exposed Omicron. Individuals a monovalent vaccine booster or breakthrough robust levels against all variants tested; however, evaded antibodies after single BA.2 BA.5 infections. Moreover, non‐vaccinated cohort, serum demonstrated almost no cross‐neutralization activities D614G, JN.1. infections earlier These findings show that SARS‐CoV‐2‐immunity is heterogeneous, depending on emphasize importance considering immune‐backgrounds when evaluating novel

Язык: Английский

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Safety and immunogenicity of a third COVID-19 vaccination in patients with immune-mediated inflammatory diseases compared with healthy controls

Annals of the Rheumatic Diseases, Год журнала: 2022, Номер 82(2), С. 292 - 300

Опубликована: Сен. 15, 2022

Objectives A third COVID-19 vaccination is recommended for immunosuppressed patients. However, data on immunogenicity and safety of a in patients with immune-mediated inflammatory diseases (IMIDs) are sparse therefore addressed within this clinical trial. Methods 60 48 healthy controls (HCs) received an mRNA vaccine. The primary endpoint was defined as the presence antibody levels against receptor-binding domain (RBD)>1500 BAU/mL IMIDs versus HCs. Further endpoints included differences neutralising antibodies cellular immune responses after vaccination. Reactogenicity recorded 7 days, evaluated until week 4. Results Rate individuals anti-RBD antibodies>1500 not significantly different between HCs (91% vs 100% p=0.101). Anti-RBD were lower than (p=0.002 p=0.016, respectively). In contrast, fold increase 0 4 higher IMIDs. Treatment biological (b) disease-modifying anti-rheumatic drugs (DMARD) or combination bDMARDs conventional synthetic DMARDs associated reduced levels. Enhanced response to wild type Omicron peptide stimulation observed No serious adverse event attributed Conclusion Our trial support effects DMARD therapy should be considered. Trial registration number EudraCT No: 2021-002693-10.

Язык: Английский

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HLA Variation and SARS-CoV-2 Specific Antibody Response

Viruses, Год журнала: 2023, Номер 15(4), С. 906 - 906

Опубликована: Март 31, 2023

Differences in SARS-CoV-2-specific immune responses have been observed between individuals following natural infection or vaccination. In addition to already known factors, such as age, sex, COVID-19 severity, comorbidity, vaccination status, hybrid immunity, and duration of infection, inter-individual variations SARS-CoV-2 may, part, be explained by structural differences brought about genetic variation the human leukocyte antigen (HLA) molecules responsible for presentation antigens T effector cells. While dendritic cells present peptides with HLA class I CD8+ induce cytotoxic lymphocyte (CTLs), they II follicular helper B cell differentiation followed memory plasma maturation. Plasma then produce antibodies. Here, we review published data linking polymorphisms antibody responses. there is evidence that heterogeneity response might related variation, are conflicting findings due part study designs. We provide insight into why more research needed this area. Elucidating basis variability will help optimize diagnostic tools lead development new vaccines therapeutics against other infectious diseases.

Язык: Английский

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