Spatiotemporal local and abscopal cell death and immune responses to histotripsy focused ultrasound tumor ablation

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Янв. 23, 2023

Introduction Histotripsy is a novel focused ultrasound tumor ablation modality with potent immunostimulatory effects. Methods To measure the spatiotemporal kinetics of local andabscopal responses to histotripsy, C57BL/6 mice bearing bilateral flank B16 melanoma or Hepa1-6 hepatocellular carcinoma tumors were treated unilateral sham partial histotripsy. Treated and contralateral untreated (abscopal) analyzed using multicolor immunofluorescence, digital spatial profiling, RNA sequencing (RNASeq), flow cytometry. Results Unilateral histotripsy triggered abscopal growth inhibition. Within zone, early high mobility group box protein 1 (HMGB1) release necroptosis accompanied by immunogenic cell death transcriptional in cells innate immune activation infiltrating myeloid natural killer (NK) cells. Delayed CD8+ T intratumoral infiltration was spatiotemporally aligned cancer features ferroptosis; this effect enhanced CTLA-4 blockade recapitulated vitro when tumor-draining lymph node co-cultured Inoculation cell-free fractions generated but not radiation freeze/thaw conferred protection from challenge. Discussion We propose that may evoke necroptotic death, priming systemic adaptive ferroptotic death.

Язык: Английский

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Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA

Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)

Опубликована: Окт. 3, 2023

Incomplete radiofrequency ablation (IRFA) triggers mild protective autophagy in residual tumor cells and results an immunosuppressive microenvironment. This accelerates the recurrence of tumors causes resistance to anti-PD-1/PDL1 therapy, which bringing a great clinical challenge immunotherapy. Mild activation can promote cancer cell survival while further amplification contributes immunogenic death (ICD). To this regard, we constructed active targeting zeolitic imidazolate framework-8 (ZIF-8) nanoparticles (NPs) loaded with STF62247 or both BMS202, namely STF62247@ZIF-8/PEG-FA (SZP) STF62247-BMS202@ZIF-8/PEG-FA (SBZP) NPs. We found that SZP NPs inhibited proliferation stimulated apoptosis exposed sublethal heat stress autophagy-dependent manner. Further discovered could amplify evoke their ICD, dramatically boosted maturation dendritic (DCs). Through vaccination experiments, for first time + treatment efficiently suppress growth new establish long-term immunological memory. Furthermore, SBZP remarkably ICD cells, obviously activate anti-tumor immune microenvironment, significantly inhibit tumors. Thus, amplified coupled therapy is potentially novel strategy treating after IRFA.

Язык: Английский

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Electrochemotherapy combined with immunotherapy – a promising potential in the treatment of cancer

Frontiers in Immunology, Год журнала: 2024, Номер 14

Опубликована: Янв. 15, 2024

Electrochemotherapy is a novel, locoregional therapy that used to treat cutaneous and deep-seated tumors. The electric pulses in electrochemotherapy increase the permeability of cell membranes target lesion thus enhance delivery low-permeant cytotoxic drugs cells, leading their death. It has also been postulated acts as an situ vaccination by inducing immunogenic This turn leads enhanced systemic antitumor response, which could be further exploited immunotherapy. However, only few clinical studies have investigated role combined treatment patients with melanoma, breast cancer, hepatocellular carcinoma, squamous carcinoma. In this review, we therefore aim review published preclinical evidence on

Язык: Английский

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Quantitative SERS detection of serum protein biomarkers for assessment of tumor microwave ablation outcomes

Chemical Engineering Journal, Год журнала: 2024, Номер 496, С. 154004 - 154004

Опубликована: Июль 14, 2024

Язык: Английский

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Breast Cancer Cryoablation Fundamentals Past and Present: Technique Optimization and Imaging Pearls

Academic Radiology, Год журнала: 2023, Номер 30(10), С. 2383 - 2395

Опубликована: Июль 15, 2023

Язык: Английский

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Radiofrequency ablation for stage <IIB non‐small cell lung cancer: Opportunities, challenges, and the road ahead

Thoracic Cancer, Год журнала: 2023, Номер 14(32), С. 3181 - 3190

Опубликована: Сен. 23, 2023

Abstract Pulmonary carcinoma represents the second common cancer for human race while its mortality rate ranked first all over world. Surgery remains primary option early‐stage non‐small cell lung (NSCLC) in some surgical traditions. Nevertheless, only less than half of patients are operable subjected to limited function and multiple primary/metastatic lesions. Recent improvements minimally invasive techniques have made procedure accessible more patients, but this percentage still does not exceed half. In recent years, radiofrequency ablation (RFA), one thermal procedures, has gradually advanced treatment addition being utilized treat breast liver cancer. Several guidelines, including American College Chest Physicians (ACCP), include RFA as an with NSCLC although level evidence is mostly retrospective studies. review, we emphasize use technique provide overview indication population, prognosis status, complications. Meanwhile, advantages disadvantages proposed existing studies compared radiotherapy. Due high gene mutation immunocompetence NSCLC, there considerable challenges clinical translation combining targeted drugs or immunotherapy that field recently begun fully appreciate.

Язык: Английский

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Immunostimulatory effects of thermal ablation: Challenges and future prospects

Journal of Cancer Research and Therapeutics, Год журнала: 2024, Номер 20(2), С. 531 - 539

Опубликована: Апрель 1, 2024

ABSTRACT This literature explores the immunostimulatory effects of thermal ablation in tumor microenvironment, elucidating mechanisms such as immunogenic cell death, tumor-specific antigens, and damage-associated molecular patterns. Furthermore, it outlines critical issues associated with ablation-induced challenges offers insights into future research avenues potential therapeutic strategies.

Язык: Английский

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High-intensity focused ultrasound ablation combined with immunotherapy for treating liver metastases: A prospective non-randomized trial

PLoS ONE, Год журнала: 2024, Номер 19(7), С. e0306595 - e0306595

Опубликована: Июль 5, 2024

Purpose Given the unique features of liver, it is necessary to combine immunotherapy with other therapies improve its efficacy in patients advanced cancer liver metastases (LM). High-intensity focused ultrasound (HIFU) ablation now widely used clinical practice and can enhanced immune benefits. The study intended prospectively evaluate safety feasibility HIFU combination systemic for metastases. Methods enrolled 14 LM who received ultrasound-guided combined checkpoint inhibitors (ICIs) such as anti-programmed cell death protein 1 (anti-PD-1 agents manufactured China) at Mianyang Central Hospital. Patients were followed up adverse events (AEs) during trial, using CommonTerminology Criteria Adverse Events v5.0(CTCAE v5.0) standard. Tumour response after treatment was assessed computerized tomography. Results (age range, 35–84 years) underwent 19 metastatic sites immunotherapy. mean lesion volume 179.9 cm3 (maximum: 733.1 cm3). Median follow-up this trial 9 months (range: 3–21) months. clinically feasible acceptable patients. Conclusion This prospective confirmed that safe treating

Язык: Английский

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Endoscopic treatment of unresectable perihilar cholangiocarcinoma: beyond biliary drainage

Therapeutic Advances in Gastroenterology, Год журнала: 2025, Номер 18

Опубликована: Апрель 1, 2025

Perihilar cholangiocarcinoma (PHCC) is an aggressive biliary malignancy originating from the epithelial cells of bile duct, typically located in extrahepatic tree, proximal to cystic duct. PHCC often presents with a rapid onset jaundice. While radical surgical resection remains only curative treatment, minority patients are eligible due early metastasis and challenges associated preoperative evaluations. Comprehensive treatments, including chemotherapy, radiotherapy, targeted therapy, immunotherapy, crucial for managing PHCC. However, advanced stages, complications such as cholestatic liver injury, malnutrition, infections pose significant obstacles these treatments. Therefore, drainage (BD) essential management In addition external methods like percutaneous transhepatic (PTBD), endoscopic (EBD), particularly retrograde cholangiopancreatography (ERCP), offer effective option internal drainage, which more physiologically compatible better tolerated. Furthermore, integration various techniques has expanded beyond mere drainage. Techniques radiofrequency ablation (RFA), photodynamic therapy (PDT), ultrasound (EUS) based present new therapeutic avenues, albeit variable results. This review aims summarize current advancements ongoing debates field treatment unresectable

Язык: Английский

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Radiofrequency ablation for the management of pancreatic mass lesions

Current Opinion in Gastroenterology, Год журнала: 2023, Номер unknown

Опубликована: Апрель 25, 2023

Purpose of review Patients with pancreatic tumors may have limited treatment options. Pancreatic tumor ablation is a novel and emerging modality which can now be performed using endoscopic ultrasound (EUS) guidance. This well suited to guide energy delivery for radiofrequency (RFA) microwave ablation. These approaches provide minimally invasive, nonsurgical methods delivering ablate in situ . summarizes the current data safety profile managing cancer neuroendocrine tumors. Recent findings RFA uses thermal induce cell death by coagulative necrosis protein denaturation. Studies reported increased overall survival patients treated EUS-guided multimodality systemic approach when used palliative surgeries. Radiofrequency corollary benefits inducing an immune-modulatory effect. Tumor marker carbohydrate antigen 19–9 has been decrease response RFA. Microwave modality. Summary utilizes focal death. applied through open, laparoscopic, radiographic modalities. are allowing

Язык: Английский

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