Proteomic Characterization of Corneal Epithelial and Stromal Cell-Derived Extracellular Vesicles DOI Open Access
Vincent Yeung, Nikolay Boychev, Levi N. Kanu

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10338 - 10338

Опубликована: Сен. 26, 2024

Communication between the different layers of cornea (epithelium and stroma) is a complex, yet crucial element in corneal healing process. Upon injury, it has been reported that bi-directional cross talk epithelium stroma via vesicular secretome, namely, extracellular vesicles (EVs), can lead to accelerated wound closure upon injury. However, distinct protein markers EVs derived from human epithelial (HCE) cells, keratocytes (HCKs), fibroblasts (HCFs), myofibroblasts (HCMs) remain poorly understood. All were enriched for CD81 showed increased expression levels ITGAV FN1 HCM-EVs compared HCE- HCF-EVs. negative GM130 minimal differences biophysical properties (particle concentration, median particle size, zeta potential). At proteomic level, we show are with proteins associated fibrosis pathways, such as COL6A1, COL6A2, MMP1, MMP2, TIMP1, TIMP2, HCE-, HCK-, Interestingly, HCE-EVs express involved EIF-2 signaling pathway (stress-induced signals regulate mRNA translation), RPS21, RALB, EIF3H, RALA, others, HCF-, HCM-EVs. In this study, isolated cell-conditioned media HCE, HCKs, HCFs, HCMs characterized their composition by Western blot, nanoparticle tracking analysis, proteomics. This study supports view have molecular may provide novel distinguish difference

Язык: Английский

Cell therapy in the cornea: The emerging role of microenvironment DOI
Giuseppe Suanno, Vincenzo Giuseppe Genna, Eleonora Maurizi

и другие.

Progress in Retinal and Eye Research, Год журнала: 2024, Номер 102, С. 101275 - 101275

Опубликована: Май 24, 2024

Язык: Английский

Процитировано

7
Injective hydrogel encapsulating dental pulp stem cells for the treatment of traumatic optic nerve injury DOI Creative Commons
Lihua Luo, Zhenjie Xing, Li Yao

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Фев. 25, 2025

The study aimed to evaluate the effect of GeLMA/bFGF hydrogel loaded with dental pulp stem cells (DPSCs) on repair and regeneration traumatic optic nerve injury. was photo-cross-linked by LED light. physical-chemical properties cytocompatibility after being squeezed (GeLMA/bFGF-SQ) were evaluated SEM degradation analyses, as well live/dead CCK-8 assays, respectively. axon growth PC12 MAP2 staining. GeLMA/bFGF/DPSCs-SQ injected in situ into lesion site observe number retinal ganglion (RGCs) calculated βIII-tubulin length regenerated axons observed CTB Tissue recovery HE TEM GeLMA/bFGF-SQ had a porous structure great degradability, good cytocompatibility. Meanwhile, DPSCs-conditioned medium (DPSCs-CM) could promote cells. Moreover, RGCs highest group. data revealed abundant newly orderly arrangement that damaged area Transplantation provided an appropriate microenvironment for injured nerves. DPSCs combined bFGF protected from apoptosis promoted secreting series neurotrophic factors.

Язык: Английский

Процитировано

0
Extracellular Vesicles and Diabetic Retinopathy: Nano-sized Vesicles With Mega-sized Hopes DOI Creative Commons
Samar Habib, Alaa Mansour, Babak Baban

и другие.

IntechOpen eBooks, Год журнала: 2024, Номер unknown

Опубликована: Июнь 24, 2024

Diabetic retinopathy (DR) is a major health problem, being the leading cause of vision loss in working-age group. Novel discoveries facilitating early diagnosis and curative treatment DR are still greatly needed. Extracellular vesicles (EVs) nano-sized released from different kinds body cells including various retinal for intercellular communication. EVs recently attracted attention because their non-immunogenic, biocompatible, biodegradable characteristics. Recent studies investigated EV cargoes patient samples, animal models, vitro models DR. Although thorough understanding trafficking retina needs extensive investigations, these will pave way discovery novel biomarkers Furthermore, EVs-based therapeutics could be great interest They presented as promising delivery tools both pharmacological genetic therapies. Here, we highlighted that discussed role inflammation angiogenesis two main pathophysiological events occurring We also tried to portray what know so far about diagnostic, prognostic, therapeutic tool.

Язык: Английский

Процитировано

0
Proteomic Characterization of Corneal Epithelial and Stromal Cell-Derived Extracellular Vesicles DOI Open Access
Vincent Yeung, Nikolay Boychev, Levi N. Kanu

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10338 - 10338

Опубликована: Сен. 26, 2024

Communication between the different layers of cornea (epithelium and stroma) is a complex, yet crucial element in corneal healing process. Upon injury, it has been reported that bi-directional cross talk epithelium stroma via vesicular secretome, namely, extracellular vesicles (EVs), can lead to accelerated wound closure upon injury. However, distinct protein markers EVs derived from human epithelial (HCE) cells, keratocytes (HCKs), fibroblasts (HCFs), myofibroblasts (HCMs) remain poorly understood. All were enriched for CD81 showed increased expression levels ITGAV FN1 HCM-EVs compared HCE- HCF-EVs. negative GM130 minimal differences biophysical properties (particle concentration, median particle size, zeta potential). At proteomic level, we show are with proteins associated fibrosis pathways, such as COL6A1, COL6A2, MMP1, MMP2, TIMP1, TIMP2, HCE-, HCK-, Interestingly, HCE-EVs express involved EIF-2 signaling pathway (stress-induced signals regulate mRNA translation), RPS21, RALB, EIF3H, RALA, others, HCF-, HCM-EVs. In this study, isolated cell-conditioned media HCE, HCKs, HCFs, HCMs characterized their composition by Western blot, nanoparticle tracking analysis, proteomics. This study supports view have molecular may provide novel distinguish difference

Язык: Английский

Процитировано

0