Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunity

Cell Reports Medicine, Год журнала: 2025, Номер unknown, С. 102028 - 102028

Опубликована: Март 1, 2025

Язык: Английский

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Huang-qin Decoction alleviates Deoxycholic Acid-induced Colorectal Cancer in Mice by Regulating Gut Microbiota

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119715 - 119715

Опубликована: Март 1, 2025

Huangqin Decoction (HQD), a traditional Chinese medicine (TCM) formula documented in Shang Han Lun, has demonstrated safety and efficacy the treatment of ulcerative colitis (UC). Recent studies also suggest that HQD exerts therapeutic effects on colorectal cancer (CRC). However, underlying mechanisms remain unclear. This study aimed to investigate CRC explore its potential action. The active ingredients targets were identified through network pharmacology-based analyses. CRC-related compared with those HQD. Shared subjected Gene Ontology (GO) functional enrichment Kyoto Encyclopedia Genes Genomes (KEGG) pathway analyses, protein-protein interaction (PPI) was constructed. Additionally, APCmin/+ mice treated 0.2 % deoxycholic acid (DCA) gavaged low or high doses Tumor morphology assessed using hematoxylin eosin (HE) staining. Immunohistochemical staining performed evaluate expression Ki-67, Caspase-3, MUC2 intestine. Periodic acid-Schiff (PAS) PAS-alcian blue (PAS-AB) utilized detect mucin distribution number goblet cells intestines mice. mRNA levels interleukin 6 (IL-6), mitogen-activated protein kinase 8 (MAPK8), vascular endothelial growth factor A (VEGFA), epidermal receptor (EGFR), albumin (ALB), Caspase 3 (CASP3) quantified quantitative reverse-transcription PCR (qRT-PCR). Immunofluorescence employed assess degree apoptosis. 16S ribosomal RNA gene sequencing, sequence curation annotation, metagenomic sequencing analyze changes composition mouse intestinal microbiota related functions signaling pathways. identified. GO KEGG analyses indicated shared primarily involved tumor suppression. effectively DCA-induced Furthermore, positive PAS PAS-AB significantly increased enhanced abundance Lachnospiraceae, Firmicutes, Fusobacteria, Clostridium, while reducing Eggerthellales. modulated secondary bile metabolism, carbohydrate synthesis, other energy metabolism pathways, which may underlie effects. mice, action be regulation gut microbiota.

Язык: Английский

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Suppressed intestinal secondary bile acids in moxifloxacin-induced hyperglycemia: studies in normal and diabetic GK rats

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Апрель 4, 2025

Objective Moxifloxacin (MFLX) frequently induces dysglycemia when used in the treatment of infectious diseases, particularly patients with diabetes. However, mechanism through which MFLX affects host glucose metabolism remains unclear. This study aimed to investigate possible underlying MFLX-induced hyperglycemia. Methods In this study, we investigated short-term (3 days) and long-term (14 effects on normal type 2 diabetic GK rats. After oral administration 40 mg/kg MFLX, blood glucose, insulin, GLP-1, fibroblast growth factor 15 (FGF15) levels rats, as well bile acids both feces, gut microbiota, were examined. Liver ileum tissues promptly harvested for detecting expression hepatic 7α-hydroxylase (CYP7A1) intestinal Takeda G-protein-coupled receptor 5 (TGR5) farnesoid X (FXR). addition, explored effect secondary (SBAs) GLP-1 secretion NCI-H716 cells, observed direct CYP7A1 HepG2 cells TGR5, FXR cells. Results It was demonstrated that induced hyperglycemia a more pronounced reduction serum FGF15 than Gut microbiota associated SBAs significantly reduced, leading decreased deoxycholic acid (DCA) lithocholic (LCA). vitro studies revealed DCA LCA (25 μM, 50 100 μM) promoted concentration-dependent manner Meanwhile, TGR5 downregulated, whereas liver increased rats after treatment. itself (0.1 1 10 did not directly altered or expressions nor it alter indicated impact primarily by changes resulting from alterations microbiota. Conclusion Our showed likely cause states highlighted critical role microbiota-SBAs-TGR5/FXR pathway

Язык: Английский

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Composites of graphene oxide and gold Nanospheres: A dual-targeted Recognition and dual-responsive release Chemophotothermal therapeutic platform

Advanced Powder Technology, Год журнала: 2025, Номер 36(6), С. 104874 - 104874

Опубликована: Апрель 5, 2025

Язык: Английский

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Vegetable oil intake:The distinctive trilateral relationship of bile acid, gut microbiota and health

Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 105001 - 105001

Опубликована: Апрель 1, 2025

Язык: Английский

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