Journal of Personalized Medicine,
Год журнала:
2023,
Номер
13(12), С. 1632 - 1632
Опубликована: Ноя. 22, 2023
Stem
cells
are
undifferentiated
presenting
extensive
self-renewal
features
and
the
ability
to
differentiate
"in
vitro"
vivo"
into
a
range
of
lineage
cells,
like
chondrogenic,
osteogenic
adipogenic
lineages
when
cultured
in
specific
inducing
media.
Two
major
domains
clinical
applications
stem
thoracic
surgery
have
been
investigated:
regenerative
medicine,
which
is
section
translational
research
tissue
engineering
focusing
on
replacement,
renewal
or
regeneration
tissues
organs
re-establish
damaged
physiologic
functions;
drug
loading
delivery,
representing
new
branch
proposing
as
carriers
provide
selected
districts
with
anti-cancer
agents
for
targeted
treatments.
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 26, 2025
Asthma
is
a
prevalent
respiratory
disease,
and
its
management
remains
largely
unsatisfactory.
Mesenchymal
stem
cells
(MSCs)
have
been
demonstrated
to
be
efficacious
in
reducing
airway
inflammation
experimental
allergic
diseases,
representing
potential
alternative
treatment
for
asthma.
Migrasomes
are
recently
identified
extracellular
vesicles
(EVs)
generated
migrating
facilitate
intercellular
communication.
The
objective
of
this
study
was
investigate
the
therapeutic
effects
migrasomes
obtained
from
MSC
model
produced
by
human
umbilical
cord
MSCs
(hUCMSCs)
were
isolated
sequential
centrifugation.
Characterization
hUCMSC-derived
carried
out
transmission
electron
microscopy
western
blot
analysis.
on
ovalbumin
(OVA)-induced
asthmatic
mice
evaluated
hematoxylin-eosin
(HE)
periodic-acid
schiff
(PAS)
staining,
their
mechanism
further
testified
immunofluorescent
real-time
PCR
flow
cytometry.
Here,
we
showed
that
inhibition
migrasomes'
production
dramatically
impaired
anti-inflammatory
hUCMSCs
OVA
animals,
as
evidenced
notable
increase
both
infiltration
inflammatory
number
epithelial
goblet
cells.
We
successfully
hUCMSC-migrasomes,
which
morphologically
intact
positive
specific
markers.
administration
hUCMSC-migrasomes
observed
significantly
ameliorate
symptoms
mucus
mice.
Additionally,
expression
Th2
cytokines
(IL-4,
IL-5
IL-13)
found
reduced,
while
activation
dendritic
(DCs)
inhibited.
HUCMSC-migrasomes
could
possibly
delivered
lung
region
after
injection,
able
taken
DCs
vivo
vitro.
Notably,
vitro,
migraosmes
decreased
capacity
BMDCs
stimulate
OVA-specific
Th2-cell
responses.
More
importantly,
adoptive
transfer
hUCMSC-migrasomes-treated
sufficient
protect
inflammation.
In
addition,
inhibited
receptor
advanced
glycation
end-products
(RAGE)
signal
OVA-treated
vitro
asthma
vivo.
Our
results
provided
first
evidence
possess
properties
OVA-induced
mice,
may
provide
novel
"MSC-cell
free"
agent
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Май 1, 2024
Abstract
Stem
cells
(SCs)
have
been
used
therapeutically
for
decades,
yet
their
applications
are
limited
by
factors
such
as
the
risk
of
immune
rejection
and
potential
tumorigenicity.
Extracellular
vesicles
(EVs),
a
key
paracrine
component
stem
cell
potency,
overcome
drawbacks
cell-free
therapeutic
agent
play
an
important
role
in
treating
various
diseases.
However,
EVs
derived
from
two-dimensional
(2D)
planar
culture
SCs
low
yield
face
challenges
large-scale
production,
which
hinders
clinical
translation
EVs.
Three-dimensional
(3D)
culture,
given
its
ability
to
more
realistically
simulate
vivo
environment,
can
not
only
expand
large
quantities,
but
also
improve
activity
EVs,
changing
content
improving
effects.
In
this
review,
we
briefly
describe
advantages
EV-related
applications,
provide
overview
3D
finally
focus
on
specific
future
perspectives
different
SCs.
Graphical
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(17), С. 9147 - 9147
Опубликована: Авг. 23, 2024
Respiratory
diseases,
including
chronic
obstructive
pulmonary
disease
(COPD),
asthma,
lung
cancer,
and
coronavirus
pneumonia,
present
a
major
global
health
challenge.
Current
diagnostic
therapeutic
options
for
these
diseases
are
limited,
necessitating
the
urgent
development
of
novel
biomarkers
strategies.
In
recent
years,
microRNAs
(miRNAs)
within
extracellular
vesicles
(EVs)
have
received
considerable
attention
due
to
their
crucial
role
in
intercellular
communication
progression.
EVs
membrane-bound
structures
released
by
cells
into
environment,
encapsulating
variety
biomolecules
such
as
DNA,
RNA,
lipids,
proteins.
Specifically,
miRNAs
EVs,
known
EV-miRNAs,
facilitate
regulating
gene
expression.
The
expression
levels
can
reflect
distinct
states
significantly
influence
immune
cell
function,
airway
inflammation,
remodeling,
proliferation,
angiogenesis,
epithelial-mesenchymal
transition,
other
pathological
processes.
Consequently,
EV-miRNAs
profound
impact
on
onset,
progression,
responses
respiratory
with
great
potential
management.
Synthesizing
current
understanding
COPD,
this
review
aims
explore
targets
examine
prospects
diagnosis
treatment
diseases.
In
the
domain
of
respiratory
illnesses,
asthma
remains
a
critical
obstacle.
The
heterogeneous
nature
this
chronic
inflammatory
disease
poses
challenges
during
its
treatment.
Glucocorticoid-based
combination
drug
therapy
now
dominates
clinical
treatments
for
asthma;
however,
glucocorticoid
resistance,
numerous
adverse
effects,
incidence
inadequate
delivery,
and
other
factors
need
development
more
effective
therapies.
recent
years,
there
has
been
extensive
research
on
nanotechnology
in
medicine.
It
shown
studies
that
these
delivery
systems
can
greatly
enhance
targeting
bioavailability
decrease
toxicity
medication.
Nanoparticle
offer
improved
therapeutic
efficacy
compared
to
conventional
administration
techniques.
Nanotechnology
enables
advancements
precision
medicine,
offering
benefits
conditions
such
as
asthma.
This
review
will
examine
consider
when
formulating
medications,
well
role
nanomaterials
their
mechanisms
action
pulmonary
medicine
World Journal of Stem Cells,
Год журнала:
2025,
Номер
17(2)
Опубликована: Фев. 24, 2025
The
global
incidence
of
asthma,
a
leading
respiratory
disorder
affecting
more
than
235
million
people,
has
dramatically
increased
in
recent
years.
Characterized
by
chronic
airway
inflammation
and
an
imbalanced
response
to
airborne
irritants,
this
condition
is
associated
with
elevated
levels
inflammatory
factors
symptoms
such
as
dyspnea,
cough,
wheezing,
chest
tightness.
Conventional
asthma
therapies,
corticosteroids,
long-acting
β-agonists,
anti-inflammatory
agents,
often
evoke
diverse
adverse
reactions
fail
reduce
hospitalization
rates
over
the
long
term
effectively.
These
limitations
have
prompted
researchers
explore
innovative
therapeutic
strategies,
including
stem
cell-related
interventions,
offering
hope
those
afflicted
incurable
disease.
In
review,
we
describe
characteristics
cells
critically
assess
potential
challenges
cell-based
therapies
improve
disease
management
treatment
outcomes
for
other
diseases.
Cells,
Год журнала:
2025,
Номер
14(7), С. 542 - 542
Опубликована: Апрель 3, 2025
Asthma
is
a
complex,
multifactorial
inflammatory
disorder
of
the
airways,
characterized
by
recurrent
symptoms
and
variable
airflow
obstruction.
So
far,
two
main
asthma
endotypes
have
been
identified,
type
2
(T2)-high
or
T2-low,
based
on
underlying
immunological
mechanisms.
Recently,
extracellular
vesicles
(EVs),
particularly
exosomes,
gained
increasing
attention
due
to
their
pivotal
role
in
intercellular
communication
distal
signaling
modulation.
In
context
pathobiology,
an
amount
experimental
evidence
suggests
that
EVs
secreted
eosinophils,
mast
cells,
dendritic
T
neutrophils,
macrophages,
epithelial
cells
contribute
disease
This
review
explores
profiling
molecular
signatures
T2-high
T2-low
asthma,
offering
novel
perspectives
mechanisms
potential
therapeutic
targets.
Biomedicines,
Год журнала:
2024,
Номер
12(6), С. 1369 - 1369
Опубликована: Июнь 20, 2024
Multipotent
mesenchymal
stromal
cells
(MSCs)
hold
promises
for
cell
therapy
and
tissue
engineering
due
to
their
self-renewal
differentiation
abilities,
along
with
immunomodulatory
properties
trophic
factor
secretion.
Extracellular
vesicles
(EVs)
from
MSCs
offer
similar
therapeutic
effects.
However,
are
heterogeneous
lead
variable
outcomes.
In
vitro
priming
enhances
MSC
performance,
improving
immunomodulation,
angiogenesis,
proliferation,
regeneration.
Various
stimuli,
such
as
cytokines,
growth
factors,
oxygen
tension,
can
prime
MSCs.
Two
classical
methods,
interferon-gamma
(IFN-γ)
hypoxia,
enhance
although
standardized
protocols
lacking.
This
review
discusses
protocols,
highlighting
the
most
commonly
used
concentrations
durations,
mechanisms
in
vivo
therapeutics
effects
of
primed
EVs.
The
feasibility
up-scaling
production
was
also
discussed.
concluded
that
IFN-γ
or
hypoxia
(alone
combination
other
factors)
boosted
immunomodulation
capability
EVs,
primarily
via
JAK/STAT
PI3K/AKT
Leptin/JAK/STAT
TGF-β/Smad
signalling
pathways,
respectively.
Incorporating
EV
enables
translation
into
cell-based
cell-free
therapies
various
disorders.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 10941 - 10959
Опубликована: Окт. 1, 2024
Nebulization
of
hypoxic
human
umbilical
cord
mesenchymal
stem
cell-derived
extracellular
vesicles
(Hypo-EVs)
can
suppress
airway
inflammation
and
remodeling
in
a
chronic
asthmatic
mouse;
however,
the
exact
mechanism
remains
unclear.
Recently,
epithelial
barrier
defects
have
been
regarded
as
crucial
therapeutic
targets
asthma.
The
aim
this
study
was
to
investigate
whether
how
Hypo-EVs
protect
against
disruption
under
conditions.
