Frontiers in Medicine,
Год журнала:
2023,
Номер
10
Опубликована: Сен. 28, 2023
Ex
vivo
organ
cultures
(EVOC)
were
recently
optimized
to
sustain
cancer
tissue
for
5
days
with
its
complete
microenvironment.
We
examined
the
ability
of
an
EVOC
platform
predict
patient
response
therapy.A
multicenter,
prospective,
single-arm
observational
trial.
Samples
obtained
from
patients
newly
diagnosed
bladder
who
underwent
transurethral
resection
tumor
and
core
needle
biopsies
metastatic
cancer.
The
tumors
cut
into
250
μM
slices
cultured
within
24
h,
then
incubated
96
h
vehicle
or
intended
treat
drug.
fixed
stained
analyze
their
morphology
cell
viability.
Each
was
given
a
score
based
on
viability,
level
damage,
Ki67
proliferation,
scores
correlated
patients'
clinical
assessed
by
pathology
Response
Evaluation
Criteria
in
Solid
Tumors
(RECIST).The
microenvironment,
including
endothelial
immune
cells,
preserved
at
high
viability
continued
division
days,
demonstrating
active
signaling
dynamics.
A
total
34
samples
tested
results.
higher
better
response.
system
showed
predictive
specificity
77.7%
(7/9,
95%
CI
0.4-0.97)
sensitivity
96%
(24/25,
0.80-0.99).EVOC
predicting
therapy
among
muscle-invasive
other
solid
tumors.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(13), С. 7475 - 7475
Опубликована: Июль 8, 2024
The
impact
of
the
HER4
receptor
on
growth
and
treatment
estrogen
receptor-positive
breast
cancer
is
widely
uncertain.
Using
CRISPR/Cas9
technology,
we
generated
stable
knockout
variants
derived
from
HER4-positive
MCF-7,
T-47D,
ZR-75-1
cell
lines.
We
investigated
tumor
proliferation
as
well
cellular
molecular
mechanisms
tamoxifen,
abemaciclib,
AMG232,
NRG1
treatments
a
function
in
vitro.
differentially
affects
response
to
tamoxifen
abemaciclib
treatment.
Most
conspicuous
increased
sensitivity
MCF-7
vitro
upon
inhibition
by
NRG1.
Additionally,
assessed
immunological
effects
responses
therapy
humanized
mice
(HTM)
based
HER4-wildtype
corresponding
HER4-knockout
cells.
Without
any
treatment,
enhanced
HTM
suggests
tumor-suppressive
effect
under
preclinical
but
human-like
conditions.
This
phenomenon
associated
with
an
HER2
expression
vivo.
Independent
HER4,
considerably
inhibited
these
mice.
However,
abemaciclib-treated
hormone
patients
tumor-associated
mdm2
gene
copy
gains
or
pronounced
showed
reduced
event-free
survival.
Evidently,
presence
efficacy
different
cells,
even
extents,
unfavorable
outcomes
patients.
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Авг. 13, 2024
Organoid
technology
has
been
developed
rapidly
in
the
past
decade,
which
involves
exploration
of
mechanism
development,
regeneration
and
various
diseases,
intersects
among
multiple
disciplines.
Thousands
literature
on
3D-culture
or
organoids
have
published
research
areas
cell
biology
tissue
engineering,
nanoscience,
oncology
so
on,
resulting
it
being
challenging
for
researchers
to
timely
summarize
these
studies.
Bibliometric
statistics
is
a
helpful
way
help
clarify
above
issues
efficiently
manage
whole
landscape
systematically.
In
our
study,
all
original
articles
were
included
Web
Science
database
from
January
2009
May
2024,
related
information
was
collected
analyzed
using
Excel
software,
“bibliometrix”
packages
R
VOSviewer
CiteSpace.
As
results,
total
6222
papers
classify
status
quo
predict
future
areas.
Our
findings
highlight
growing
trend
publications
organoids,
with
United
States
Netherlands
leading
this
field.
The
University
California
System,
Harvard
University,
Utrecht
Medical
Center
emerged
as
pivotal
contributors
key
authors
field
include
Clevers,
H,
Beekman,
JM
Spence
JR.
results
also
revealed
that
hotspots
trends
mainly
focused
clinical
treatment,
drug
screening,
application
materials
technologies
such
“hydrogel”
“microfluidic
technology”
organoids.
Next,
we
had
an
in-depth
interpretation
development
process
organoid
area,
including
emergence
technology,
translation
bench
bedsides,
profiles
most
widely
studied
types
technologies,
emerging
organoid-immune
co-cultures
trends.
Furthermore,
discussed
pitfalls,
challenges
prospects
technology.
conclusion,
study
provides
readers
straightforward
convenient
access
Frontiers in Oncology,
Год журнала:
2023,
Номер
13
Опубликована: Сен. 15, 2023
In
the
last
three
decades,
there
has
been
a
surge
in
research
on
cancer
organoids
using
3D
culture
technologies,
which
resulted
development
of
physiological
human
models.
This
study
aims
to
provide
an
overview
global
trends
and
frontiers
organoids.A
total
3189
publications
from
1991
2021
were
collected
Science
Citation
Index-Expanded
(SCIE)
Web
(WoS).
Bibliometric
methods
such
as
R
package
"Bibliometrix,"
Citespace,
VOS
viewer
software
employed
investigate
visualize
bibliographic
coupling,
co-citation,
co-authorship,
co-occurrence
trends,
well
publication
field
research.From
2021,
significant
increase
organoids,
with
most
articles
being
North
America,
Eastern
Asia,
Western
Europe.
The
USA
had
highest
number
publications,
citations,
prolific
authors,
funding
globally.
Cancers
was
journal
while
Nature
best
link
strength.
Harvard
University
contributive
institutions.
this
could
be
classified
into
five
clusters:
chemotherapy
study,
for
drug
screening,
different
models,
molecular
mechanism
organoid
construction.
These
areas
are
expected
remain
hotspots
future
research.The
is
based
current
trends.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 26, 2024
Breast
cancer
is
the
most
common
malignant
tumor
in
women.
Due
to
high
heterogeneity
of
breast
cells,
traditional
vitro
research
models
still
have
major
limitations.
Therefore,
it
urgent
establish
an
experimental
model
that
can
accurately
simulate
characteristics
human
cancer.
organoid
technology
emerged
as
times
required,
is,
construct
tissue
analogs
with
organ
by
using
a
patient’s
through
3D
culture
.
Since
fully
preserve
histology
and
genetic
original
tumor,
provides
reliable
for
preclinical
drug
screening,
establishment
biobanks,
into
mechanisms
development,
determination
targets.
It
has
promoted
personalized
treatment
clinical
patients.
This
article
mainly
focuses
on
recent
progress
applications
cancer,
discussing
current
limitations
prospects
technology.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(22), С. 12277 - 12277
Опубликована: Ноя. 15, 2024
Head
and
neck
squamous
cell
carcinoma
(HNSCC)
is
highly
enriched
with
tumor-infiltrating
myeloid
cells,
including
tumor-associated
macrophages
(TAMs)
myeloid-derived
suppressor
cells
(MDSCs).
However,
effective
therapeutic
agents
targeting
in
HNSCC
are
currently
lacking.
Here,
we
employed
a
unique
co-culture
system
to
investigate
how
affect
cells.
We
found
that
the
presence
of
cancer
significantly
enhances
proliferation
promotes
TAM
differentiation.
To
identify
potential
agents,
screened
custom
library
70
kinase
inhibitors
assess
their
effects
on
distinct
subsets
discovered
specific
differentially
suppressed
populations
TAMs,
monocytic
MDSCs
(M-MDSCs),
or
polymorphonuclear
(PMN-MDSCs),
suggesting
inhibiting
different
targets
could
reduce
Conversely,
some
were
increase
population
CD11b+Ly6G−Ly6C−
Among
promising
tested,
vatalanib,
VEGF-R
inhibitor,
demonstrated
significant
vivo
efficacy
at
tumor
growth
reducing
thereby
underscoring
its
as
agent.
Our
findings
highlight
differential
modulatory
HNSCC-associated
caution
application
anti-cancer
drugs.
This
experimental
may
provide
robust
platform
for
identifying
new
beyond,
elucidating
mechanistic
insights
into
tumor-myeloid
interaction.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 10, 2024
Triple-negative
breast
cancer
(TNBC)
stands
as
the
most
complex
and
daunting
subtype
of
affecting
women
globally.
Regrettably,
treatment
options
for
TNBC
remain
limited
due
to
its
clinical
complexity.
However,
immunotherapy
has
emerged
a
promising
avenue,
showing
success
in
developing
effective
therapies
advanced
cases
improving
patient
outcomes.
Improving
treatments
involves
reducing
side
effects,
minimizing
systemic
toxicity,
enhancing
efficacy.
Unlike
traditional
immunotherapy,
engineered
nonmaterial's
can
precisely
target
TNBC,
facilitating
immune
cell
access,
antigen
presentation,
triggering
lasting
responses.
Nanocarriers
with
enhanced
sensitivity
specificity,
specific
cellular
absorption,
low
toxicity
are
gaining
attention.
Nanotechnology-driven
immunoengineering
strategies
focus
on
targeted
delivery
systems
using
multifunctional
molecules
precise
tracking,
diagnosis,
therapy
TNBC.
This
study
delves
into
TNBC's
tumour
microenvironment
(TME)
remodeling,
therapeutic
resistance,
nanotechnology.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13262 - 13262
Опубликована: Дек. 10, 2024
Lung
cancer
is
the
most
common
type
of
malignant
tumor
worldwide.
Plasma-activated
medium
(PAM)
an
innovative
treatment
method
that
has
received
considerable
scientific
attention.
The
objective
this
study
to
evaluate
effects
PAM
on
anti-tumor
characteristics
non-small
cell
lung
(NSCLC)
cells
in
two-dimensional
(2D)
and
three-dimensional
(3D)
cultures.
proliferative
migratory
capabilities
A549
2D
3D
cultures
were
assessed
using
MTT,
migration,
invasion
assays,
cycle,
respectively.
also
investigated
impact
changes
content
intracellular
extracellular
reactive
species
analyzed
protein
expression
Western
Blot
method.
inhibited
viability,
abilities
both
cultures,
suppressed
epithelial–mesenchymal
transition
(EMT)
process,
downregulated
RAS/ERK
signaling
pathway,
which
effectively
spheroid
formation.
Additionally,
effect
was
mediated
through
ROS-induced
oxidative
reactions,
exhibited
greater
cytotoxicity
culture
compared
culture.
As
2D,
model
provides
a
viable
vitro
for
studying
mechanisms
cancer.
represents
effective
new
NSCLC.
Genes,
Год журнала:
2024,
Номер
15(12), С. 1615 - 1615
Опубликована: Дек. 17, 2024
The
tumor
secretome
comprises
the
totality
of
protein
factors
secreted
by
various
cell
components
within
microenvironment,
serving
as
primary
medium
for
signal
transduction
between
cells
and
stromal
cells.
deletion
or
mutation
p53
gene
leads
to
alterations
in
cellular
secretion
characteristics,
contributing
construction
microenvironment
a
non-autonomous
manner.
This
review
discusses
critical
roles
mutant
regulating
remodel
drive
progression,
influence
plasticity
cancer-associated
fibroblasts
(CAFs)
well
dynamics
immunity
focusing
on
both
expression
pathways.
aim
is
provide
new
insights
targeted
cancer
therapies.
Frontiers in Medicine,
Год журнала:
2023,
Номер
10
Опубликована: Сен. 28, 2023
Ex
vivo
organ
cultures
(EVOC)
were
recently
optimized
to
sustain
cancer
tissue
for
5
days
with
its
complete
microenvironment.
We
examined
the
ability
of
an
EVOC
platform
predict
patient
response
therapy.A
multicenter,
prospective,
single-arm
observational
trial.
Samples
obtained
from
patients
newly
diagnosed
bladder
who
underwent
transurethral
resection
tumor
and
core
needle
biopsies
metastatic
cancer.
The
tumors
cut
into
250
μM
slices
cultured
within
24
h,
then
incubated
96
h
vehicle
or
intended
treat
drug.
fixed
stained
analyze
their
morphology
cell
viability.
Each
was
given
a
score
based
on
viability,
level
damage,
Ki67
proliferation,
scores
correlated
patients'
clinical
assessed
by
pathology
Response
Evaluation
Criteria
in
Solid
Tumors
(RECIST).The
microenvironment,
including
endothelial
immune
cells,
preserved
at
high
viability
continued
division
days,
demonstrating
active
signaling
dynamics.
A
total
34
samples
tested
results.
higher
better
response.
system
showed
predictive
specificity
77.7%
(7/9,
95%
CI
0.4-0.97)
sensitivity
96%
(24/25,
0.80-0.99).EVOC
predicting
therapy
among
muscle-invasive
other
solid
tumors.
