Pharmedicine journal.,
Год журнала:
2024,
Номер
1(1), С. 1 - 19
Опубликована: Фев. 29, 2024
Flow
cytometry,
a
pivotal
tool
in
clinical
and
research
labs
since
the
discovery
of
cell
markers
mid-1970s,
plays
crucial
role
across
all
phases
drug
discovery.
Modern
flow
cytometers
can
detect
rare
types
relevant
to
disease
pathogenesis,
measure
numerous
parameters
simultaneously,
thus,
offer
versatility
screening.
In
studies,
cytometry
contributes
assessment
pharmacokinetics,
pharmacodynamics
safety
animal
models
trials.
It
also
be
used
monitor
efficacy
identify
biomarkers
for
diagnosis
prognosis.
essence,
is
versatile,
instrumental
technique
that
supports
from
target
identification
through
development,
limited
only
by
creativity
researcher
availability
fluorescent
labels
or
specific
size/scatter
related
findings.
This
review
article
focuses
on
use
development
summarizing
not
conventional
assays
such
as
immunophenotyping,
measurement
programmed
death
pathways
division
provide
insights
into
effects
patient
responses,
but
novel
approaches
including
mass
spectral
droplet
cytometry.
Keywords:
discovery,
Transplantation,
Год журнала:
2024,
Номер
108(10), С. 2034 - 2044
Опубликована: Март 12, 2024
Transplantation
serves
as
the
cornerstone
of
treatment
for
patients
with
end-stage
organ
disease.
The
prevalence
complications,
such
allograft
rejection,
infection,
and
malignancies,
underscores
need
to
dissect
complex
interactions
immune
system
at
single-cell
level.
In
this
review,
we
discuss
studies
using
mass
cytometry
or
by
time-of-flight,
a
cutting-edge
technology
enabling
characterization
populations
cell-to-cell
in
granular
detail.
We
review
application
human
experimental
animal
context
transplantation,
uncovering
invaluable
contributions
tool
understanding
rejection
other
transplant-related
complications.
recent
innovations
that
have
potential
streamline
standardize
workflows
multisite
clinical
trials.
Additionally,
introduce
imaging
cytometry,
technique
couples
power
spatial
context,
thereby
mapping
cellular
within
tissue
microenvironments.
synergistic
integration
data
omics
sets
high-dimensional
platforms
further
define
dynamics
is
discussed.
conclusion,
technologies,
when
integrated
tools
data,
shed
light
on
intricate
landscape
response
transplantation.
This
approach
holds
significant
enhancing
patient
outcomes
advancing
our
facilitating
development
new
diagnostics
therapeutics.
Pharmedicine journal.,
Год журнала:
2024,
Номер
1(1), С. 1 - 19
Опубликована: Фев. 29, 2024
Flow
cytometry,
a
pivotal
tool
in
clinical
and
research
labs
since
the
discovery
of
cell
markers
mid-1970s,
plays
crucial
role
across
all
phases
drug
discovery.
Modern
flow
cytometers
can
detect
rare
types
relevant
to
disease
pathogenesis,
measure
numerous
parameters
simultaneously,
thus,
offer
versatility
screening.
In
studies,
cytometry
contributes
assessment
pharmacokinetics,
pharmacodynamics
safety
animal
models
trials.
It
also
be
used
monitor
efficacy
identify
biomarkers
for
diagnosis
prognosis.
essence,
is
versatile,
instrumental
technique
that
supports
from
target
identification
through
development,
limited
only
by
creativity
researcher
availability
fluorescent
labels
or
specific
size/scatter
related
findings.
This
review
article
focuses
on
use
development
summarizing
not
conventional
assays
such
as
immunophenotyping,
measurement
programmed
death
pathways
division
provide
insights
into
effects
patient
responses,
but
novel
approaches
including
mass
spectral
droplet
cytometry.
Keywords:
discovery,
