The shared circulating diagnostic biomarkers and molecular mechanisms of systemic lupus erythematosus and inflammatory bowel disease
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Май 7, 2024
Background
Systemic
lupus
erythematosus
(SLE)
is
a
multi-organ
chronic
autoimmune
disease.
Inflammatory
bowel
disease
(IBD)
common
inflammatory
of
the
gastrointestinal
tract.
Previous
studies
have
shown
that
SLE
and
IBD
share
pathogenic
pathways
genetic
susceptibility,
but
specific
mechanisms
remain
unclear.
Methods
The
datasets
were
downloaded
from
Gene
Expression
Omnibus
(GEO).
Differentially
expressed
genes
(DEGs)
identified
using
Limma
package.
Weighted
gene
coexpression
network
analysis
(WGCNA)
was
used
to
determine
co-expression
modules
related
IBD.
Pathway
enrichment
performed
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
for
co-driver
genes.
Using
Least
AbsoluteShrinkage
Selection
Operator
(Lasso)
regressionand
Support
Vector
Machine-Recursive
Feature
Elimination
(SVM-RFE),
diagnostic
markers
both
diseases
further
evaluated.
Then,
we
utilizedthe
CIBERSORT
method
assess
abundance
immune
cell
infiltration.
Finally,we
single-cell
obtain
location
markers.
Results
71
driver
in
cohorts
based
on
DEGs
module
KEGG
GO
results
showed
these
closely
associated
with
positive
regulation
programmed
death
responses.
By
LASSO
regression
SVM,
five
hub
(KLRF1,
GZMK,
KLRB1,
CD40LG,
IL-7R)
ultimately
determined
as
ROC
curve
also
good
performance.
outcomes
infiltration
demonstrated
shared
almost
identical
patterns.
Furthermore,
majority
commonly
NK
cells
by
analysis.
Conclusion
This
study
demonstrates
pathways.
In
addition,
show
similar
cellinfiltration
microenvironments
which
provides
newperspectives
future
treatment.
Язык: Английский
Bioinformatics Approach to Identify the Relationship among COVID-19, Acute Myocardial Infarction, and Acute Ischemic Stroke
Next research.,
Год журнала:
2025,
Номер
unknown, С. 100178 - 100178
Опубликована: Янв. 1, 2025
Язык: Английский
Differential DNA methylation associated with delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage: a systematic review
Neurosurgical Review,
Год журнала:
2024,
Номер
47(1)
Опубликована: Апрель 10, 2024
Abstract
Recent
studies
suggest
that
differential
DNA
methylation
could
play
a
role
in
the
mechanism
of
cerebral
vasospasm
(CVS)
and
delayed
ischemia
(DCI)
after
aneurysmal
subarachnoid
hemorrhage
(aSAH).
Considering
significance
this
matter
lack
effective
prophylaxis
against
DCI,
we
aim
to
summarize
current
state
knowledge
regarding
their
associations
with
identify
gaps
for
future
trial.
PubMed
MEDLINE,
Scopus,
Web
Science
were
searched
by
two
authors
three
waves
relevant
association
DCI
aSAH.
PRISMA
checklist
was
followed
systematic
structure.
STROBE
statement
used
assess
quality
risk
bias
within
studies.
This
research
funded
National
Centre,
Poland
(grant
number
2021/41/N/NZ2/00844).
Of
70
records,
7
peer-reviewed
articles
met
eligibility
criteria.
Five
candidate
gene
approach,
epigenome-wide
(EWAS),
one
utilized
bioinformatics
previous
EWAS,
using
more
than
approach.
Methylation
status
four
cytosine-guanine
dinucleotides
(CpGs)
related
distinct
genes
(ITPR3,
HAMP,
INSR,
CDHR5)
have
been
found
significantly
or
suggestively
associated
Analysis
epigenetic
clocks
yielded
significant
lower
age
acceleration
radiological
CVS
but
not
DCI.
Hub
hypermethylation
(VHL,
KIF3A,
KIFAP3,
RACGAP1,
OPRM1)
hypomethylation
(ALB,
IL5)
indicated
through
analysis.
As
none
CpGs
overlapped
across
studies,
meta-analysis
applicable.
The
identified
sites
might
potentially
serve
as
biomarker
early
diagnosis
aSAH
future.
However,
overlapping
results
prompts
need
large-scale
multicenter
Challenges
prospects
are
discussed.
Язык: Английский
Effects of PGK1 on immunoinfiltration by integrated single-cell and bulk RNA-sequencing analysis in sepsis
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 6, 2024
Sepsis,
a
life-threatening
organ
dysfunction
caused
by
dysregulated
immune
response
to
infection,
remains
significant
global
health
challenge.
Phosphoglycerate
kinase
1
(PGK1)
has
been
implicated
in
regulating
inflammation
and
cell
infiltration
inflammatory
conditions.
However,
the
role
of
PGK1
sepsis
largely
unexplored.
Four
microarray
datasets
high
throughput
sequencing
dataset
were
acquired
from
GEO
database
reveal
expression
patients
sepsis.
Quantitative
real-time
PCR
western
blotting
was
then
used
validate
level.
Additionally,
single-cell
RNA
data
integration,
including
gene
set
enrichment
analysis
(GSEA),
KEGG
GO
functional
analysis,
performed
elucidate
Our
results
revealed
upregulation
patients,
with
area
under
ROC
curve
(AUC)
exceeding
0.9
across
multiple
datasets,
indicating
PGK1's
strong
potential
as
diagnostic
biomarker.
Notably,
enriched
key
immune-related
pathways,
TNF
signaling
leukocyte
transendothelial
migration,
suggesting
its
involvement
regulation.
Furthermore,
showed
positive
correlation
levels
mediators
CXCL1,
CXCL16,
chemokine
receptor
CCR1.
In
terms
infiltration,
positively
correlated
naive
B
cells,
resting
memory
CD4
T
cell,
gamma
delta
M0
macrophages,
eosinophils
negatively
plasma
CD8
activated
Tregs,
dendritic
cells.
This
study
concluded
that
served
novel
biomarker
for
sepsis,
implications
prognosis
The
association
pathways
types
highlight
pathogenesis
Язык: Английский
SARS-CoV-2 N protein induces alveolar epithelial apoptosis via NLRP3 pathway in ARDS
International Immunopharmacology,
Год журнала:
2024,
Номер
144, С. 113503 - 113503
Опубликована: Ноя. 25, 2024
Язык: Английский
Advancements in omics technologies: Molecular mechanisms of acute lung injury and acute respiratory distress syndrome (Review)
International Journal of Molecular Medicine,
Год журнала:
2024,
Номер
55(3)
Опубликована: Дек. 27, 2024
Acute
lung
injury
(ALI)/acute
respiratory
distress
syndrome
(ARDS)
is
an
inflammatory
response
arising
from
and
systemic
with
diverse
causes
associated
high
rates
of
morbidity
mortality.
To
date,
no
fully
effective
pharmacological
therapies
have
been
established
the
relevant
underlying
mechanisms
warrant
elucidation,
which
may
be
facilitated
by
multi‑omics
technology.
The
present
review
summarizes
application
technology
in
identifying
novel
diagnostic
markers
therapeutic
strategies
ALI/ARDS
as
well
its
pathogenesis.
Язык: Английский
Application of Macrophage Subtype Analysis in Acute Lung Injury/Acute Respiratory Distress Syndrome
Frontiers in Bioscience-Landmark,
Год журнала:
2024,
Номер
29(12)
Опубликована: Дек. 6, 2024
Acute
lung
injury
(ALI)/acute
respiratory
distress
syndrome
(ARDS)
is
a
common
critical
illness.
Supportive
therapy
still
the
main
strategy
for
ALI/ARDS.
Macrophages
are
predominant
immune
cells
in
lungs
and
play
pivotal
role
maintaining
homeostasis,
regulating
metabolism,
facilitating
tissue
repair.
During
ALI/ARDS,
these
versatile
undergo
polarization
into
distinct
subtypes
with
significant
variations
transcriptional
profiles,
developmental
trajectory,
phenotype,
functionality.
This
review
discusses
developments
analysis
of
alveolar
macrophage
study
potential
value
targeting
new
diagnosis,
prognostic
evaluation,
treatment
Язык: Английский