Integrating bioinformatics and machine learning for comprehensive analysis and validation of diagnostic biomarkers and immune cell infiltration characteristics in pediatric septic shock

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 26, 2025

This study aims to predict and diagnose pediatric septic shock through the screening of immune infiltration-related biomarkers. Three gene expression datasets were accessible from Gene Expression Omnibus repository. The differentially expressed genes identified using R 4.3.2 ( https://www.r-project.org/ ), followed by set enrichment analysis. Thereafter, utilizing machine-learning algorithms. receiver operating characteristic curve was employed assess discrimination effectiveness hub genes. inflammatory status evaluated cell-type identification estimating relative subsets RNA transcripts (CIBERSORT). correlation between diagnostic markers infiltrating cells further examined. Overall, we detected 12 CD177, MCEMP1, MMP8, OLAH examined as indicators for shock, revealing statistically significant differences (P < 0.01) efficacy in validation cohort. cell infiltration analysis suggests that various may contribute onset shock. Furthermore, all characteristics exhibit varying degrees with cells. identifies four potential biomarkers—CD177, OLAH—that provide value novel insights into dysregulation Through integration bioinformatics machine learning methodologies, offer a perspective on mechanisms involved potentially facilitating more targeted personalized therapies individual patients.

Язык: Английский

---

Diagnostic Accuracy of Septicyte Rapid to Discriminate Sepsis From Sterile Inflammation in Patients Meeting Sepsis Criteria According to Sepsis-3 Definition at ICU Admission

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

Purpose: The aim of this study was to validate the SeptiCyte® RAPID assay, a molecular test distinguish sepsis from sterile inflammation, by determining its diagnostic accuracy in critically ill patients who meet criteria for according Sepsis-3 definition on ICU admission. Methods: This is an observational, prospective, and multicenter carried out seven hospitals Andalusia (Spain). A 2.5 mL whole blood sample collected tested SeptiCyte kit real time PCR platform (Idylla^TM). score 0 15 (SeptiScore™) generated that falls into four bands based increasing likelihood infection-positive systemic inflammation. Results: We enrolled 354 patients, whom 86 (24.3%) did not present at researchers´ discretion. showed AUC [0.84 (CI95% 0.79-0.87)] differentiating significantly better diagnosis than CRP [0.75 0.70-0.80)] (p =0.003) but without significant differences with PCT [0.80 0.75-0.84)]. SeptiScore distribution higher PPV 68.8% 92.2% (Bands 3 4) 100% inflammation (Band 1). Independent risk factors were estimated probability [OR 8.02 (CI 95% 4.50-14.28), p<0.001], SeptiScore 1.64 1.35-1.99), log procalcitonin 1.68 1.09-2.59), p=0.020]. Conclusion: SeptiCyte® discriminates adults, adding value sepsis.

Язык: Английский

---

Sialyl Lewis^x Glycomimetics as E- and P-Selectin Antagonists Targeting Hyperinflammation

ACS Medicinal Chemistry Letters, Год журнала: 2024, Номер 16(1), С. 64 - 71

Опубликована: Ноя. 9, 2024

Inflammatory disorders, such as sepsis, pancreatitis, and severe COVID-19, often cause immune dysfunction high mortality. These conditions trigger excessive cell influx, leading to cytokine storms, organ damage, compensatory suppression that results in immunoparalysis, dysfunction, reinfection. Controlled reversible immunosuppression limiting recruitment inflammation sites could reduce hyperinflammation prevent exhaustion. PSGL-1 on leukocytes binds vascular P- E-selectins via its sialyl Lewisx pharmacophore, triggering key features of systemic inflammatory response syndrome sepsis. We report the discovery two immunomodulators, glycomimetics (12 13), with a tetrazole carboxyl bioisostere 3a, which E-selectin blocks their interaction PSGL-1. In an vivo model, they reduced recruitment, evidenced by decreased neutrophils, CD11b+, monocytes/macrophages, PSGL-1-positive cells at various time points. may be promising leads for managing syndrome.

Язык: Английский

---

Sepsis severity in chronic intestinal failure patients presenting with catheter related blood stream infection

Intestinal failure., Год журнала: 2024, Номер 2, С. 100023 - 100023

Опубликована: Апрель 1, 2024

Язык: Английский

---

Revolutionizing cancer treatment: the rise of personalized immunotherapies

Discover Oncology, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 18, 2024

Язык: Английский

---