Comparative Immunology Microbiology and Infectious Diseases, Год журнала: 2024, Номер 116, С. 102290 - 102290
Опубликована: Дек. 13, 2024
Язык: Английский
Опубликована: Дек. 5, 2024
Язык: Английский
Процитировано
9
Poultry Science, Год журнала: 2025, Номер 104(3), С. 104847 - 104847
Опубликована: Янв. 23, 2025
Reticuloendotheliosis virus (REV) is a gamma retrovirus that can cause immunosuppression, dwarf syndrome and acute reticulocytoma in poultry. The molecular mechanism by which REV infection leads to immunosuppression tumorigenesis poorly understood. In this study, we elucidated the regulatory network of miRNA-mRNA major signaling pathways involved REV-SNV infection. Therefore, used spleen necrosis (SNV) model inoculate one-day-old specific pathogen-free (SPF) chickens then performed global miRNA mRNA expression profiling conducting high-throughput sequencing 18 bursa Fabricius samples collected at 7, 14, 21 dpi. total, 213 differentially expressed miRNAs (DEMs) 3311 genes (DEGs) were identified. constructed based on association analysis these DEMs DEGs, 1376 negatively correlated pairs identified; among them, 82 identified 7 dpi, 203 14 873 Moreover, results Kyoto Encyclopedia Genes Genomes (KEGG) enrichment mRNAs revealed greater immune-related pathways, such as immune system, signal transduction, cell growth death, molecules interactions. We confirmed selected their DEGs quantitative RT-PCR (qRT-PCR) analysis. These findings increased our understanding interactions target during with REV-SNV, contributed host-virus
Язык: Английский
Процитировано
0
Poultry Science, Год журнала: 2025, Номер 104(5), С. 104919 - 104919
Опубликована: Фев. 17, 2025
This study aimed to investigate the effects of dietary supplementation rhamnolipids (RLS) on growth performance, gut morphology, antioxidant function, lipid metabolism, immune responses, and microbiota in Linnan yellow-feathered broilers. A total 390 one-day-old broilers were randomly assigned three groups: Control (CON), 250 mg/kg RLS (RLS250), or 500 (RLS500). The fed with basal diet, diet supplemented for 56 days. Each treatment contained 10 replicates 13 chickens per replicate. Results showed that RLS250 RLS500 markedly improved final BW ADG day 56. reduced CD increased VH/CD jejunum ileum. significantly levels T-AOC GSH-Px 28, while SOD higher than CON groups also regulated metabolism by increasing concentration serum HDL-C decreased LDL-C. immunoglobulins including IgA, IgM IgY notably those Meanwhile, ileum IgA evidently other two groups. level IL-10. no significant VFA cecum 16S rRNA sequencing optimized lowering relative abundance Anaerofilum DUT089, regulating g__norank_f__ Ruminococcaceae. found intestinal broilers, revealing is potential feed additive use animal husbandry.
Язык: Английский
Процитировано
0
Veterinary Immunology and Immunopathology, Год журнала: 2025, Номер 283, С. 110933 - 110933
Опубликована: Апрель 5, 2025
Язык: Английский
Процитировано
0
Frontiers in Veterinary Science, Год журнала: 2025, Номер 12
Опубликована: Апрель 15, 2025
Infectious Bursal Disease (IBD) is an acute, highly infectious, immunosuppressive disease caused by the infectious bursal virus (IBDV). To elucidate prevalence of IBDV in southern China, a total 60 tissues (including spleen and bursa) suspected infection were collected from broiler chickens 2023. In this study, 31 strains successfully isolated. The vp2 gene sequences these isolates sequenced analysed. results showed that 8 identified as very virulent strains, 11 classical 12 novel variants. nucleotide sequence identity among ranged 90.7 to 100%, determined MegAlign. Further analysis revealed mutant exhibited characteristic amino acid sites are 252I, 254 N, 262Y, 299S 318D. Phylogenetic reference South China demonstrated strain has diverged previously prevalent such Variant E GLS, forming distinct lineage. This finding implies high mutation rate may compromise vaccine efficacy pose new challenges for prevention control poultry production.
Язык: Английский
Процитировано
0
Developmental & Comparative Immunology, Год журнала: 2024, Номер 162, С. 105280 - 105280
Опубликована: Окт. 11, 2024
Язык: Английский
Процитировано
2
Poultry Science, Год журнала: 2024, Номер 103(12), С. 104389 - 104389
Опубликована: Окт. 9, 2024
Programmed cell death protein 1 (PD-1)/PD-1 ligand (PD-L1) binding contributes to immune evasion mechanisms responsible for B lymphocyte exhaustion and apoptosis. This facilitates immunosuppression in chronic viral infections, including infectious bursal disease virus (IBDV). Our previous study showed that PD-1 PD-L1 expression increases the peripheral blood mononuclear cells of chickens infected with IBDV. However, due their high production costs immune-related adverse events, monoclonal antibodies targeting or are unsuitable therapeutic agents. Thus, current study, we designed peptides optimized sites investigated ability disrupt PD-1/PD-L1 restore function vitro. The peptide gCK-16 exhibited a affinity (K
Язык: Английский
Процитировано
1
Poultry Science, Год журнала: 2024, Номер 103(7), С. 103833 - 103833
Опубликована: Май 7, 2024
The family of cell cycle-dependent kinases (CDKs) serves as catalytic subunits within protein kinase complexes, playing a crucial role in cycle progression. While the function CDK proteins regulating mammalian innate immune responses and virus replication is well-documented, their chickens remains unclear. To address this, we cloned several chicken CDKs, specifically CDK6 through CDK10. We observed that widely expressed across various tissues, with localization cytoplasm, nucleus, or both DF-1 cells. In addition, also found multiple CDKs negatively regulate IFN-β signaling induced by MAVS STING targeting different steps. Moreover, during infection infectious bursal disease (IBDV), except CDK10, were recruited co-localized viral VP1. Interestingly, overexpression significantly enhanced IBDV replication. Conversely, knocking down led to marked increase production, triggered chMDA5. Furthermore, endogenous RNA interference substantially reduced These findings collectively suggest particularly CDK6, act suppressors production play facilitative
Язык: Английский
Процитировано
0
Comparative Immunology Microbiology and Infectious Diseases, Год журнала: 2024, Номер 116, С. 102290 - 102290
Опубликована: Дек. 13, 2024
Язык: Английский
Процитировано
0