Platelet-derived extracellular vesicles: emerging players in hemostasis and thrombosis

Journal of Liposome Research, Год журнала: 2025, Номер unknown, С. 1 - 11

Опубликована: Апрель 25, 2025

Platelets, long recognized for their role in hemostasis and thrombosis, have emerged as key players a wide array of physiological pathological processes through the release platelet-derived extracellular vesicles (PEVs). These nanoscale vesicles, rich bioactive molecules such proteins, lipids, nucleic acids, facilitate intercellular communication influence ranging from angiogenesis inflammation to immune modulation tissue repair. PEVs, most abundant circulation, display procoagulant activity 50-100 times greater than activated platelets, underscoring pivotal thrombosis. Recent research has unveiled dual health disease, highlighting potential diagnostic biomarkers therapeutic vehicles. PEVs are implicated cancer progression, autoimmune diseases, infectious where they modulate tumor microenvironments, responses, inflammatory pathways. Moreover, ability deliver agents with high specificity biocompatibility positions them promising tools regenerative medicine, drug delivery, targeted therapies. This review comprehensively explores PEV biogenesis, cargo composition, multifaceted roles well broader implications disease. It also markers innovative strategies, offering insights into application treating thrombotic disorders, cancer, diseases. Despite significant advancements, challenges remain standardizing isolation protocols translating preclinical findings clinical applications. Unlocking full promises revolutionize diagnostics therapeutics, paving way novel approaches managing complex

Язык: Английский

Human milk-derived extracellular vesicle treatment promotes the heat shock response in neonates with perinatal high fat diet exposure.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

Abstract Maternal consumption of a high-fat diet (mHFD) during perinatal life (the collective prenatal and postnatal periods) influences neonatal development, initiates hypothalamic-pituitary-adrenal (HPA) axis activation, impacts the long-term physiological metabolic health offspring. Milk-derived extracellular vesicles (MEVs) are lipid-coated nanovesicles found in mammalian milk that survive intestinal degradation cross complex biological barriers, including blood-brain barrier. MEVs have known cytoprotective activity peripheral organs; however, their pro-survival functions response to chronic pro-inflammation stemming from early nutrient stress remain unknown brain. Further, sex differences resulting MEV treatment require investigation, as male female neonates illicit variable responses stress. We investigated whether promote heat shock (HSR), principal mechanism responsible for refolding or degrading misfolded protein aggregates through action (HSP) chaperones. interaction between HSR liver, hypothalamus, prefrontal cortex rats exposed mHFD within hyporesponsive period at day 11. robustly modulated with largest recorded cortex. Specifically, cortex, led an upregulation main transcription factor (HSF1), while downregulating negative regulators HSF1 (Hsp70 Hsp90). These results suggest may influence outcomes by activating HSF1-mediated specific manner mHFD.

Язык: Английский

Stability Dynamics of Plant-Based Extracellular Vesicles Drug Delivery

Journal of Xenobiotics, Год журнала: 2025, Номер 15(2), С. 55 - 55

Опубликована: Апрель 13, 2025

Plant-based extracellular vesicles (PBEVs) have been recognized for their wide range of applications in drug delivery however, the extent medicinal applicability depends on how well they are preserved and stored. Assessing physicochemical properties, such as size, particle concentration, shape, activity cargo, forms foundation determining stability during storage. Moreover, evaluation PBEVs is essential to ensure both safety efficacy, which critical advancing clinical development. Maintaining biological EVs storage a challenging task, similar preservation cells other cell-derived products like proteins. However, despite limited studies, it expected that storing drug-loaded may present fewer challenges compared cell-based therapies, although some limitations inevitable. This article provides comprehensive overview current knowledge methods, particularly focusing role carriers. hold promise potential candidates oral administration due effective intestinal absorption ability withstand basic acidic environments. maintaining critical. this review centers isolation, characterization, PBEVs, exploring advantages offer. Furthermore, highlights key areas require further research overcome existing enhance development methods therapeutic EVs.

Язык: Английский