Therapies, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Circulation Research, Год журнала: 2025, Номер 136(11), С. 1476 - 1493
Опубликована: Май 22, 2025
Cardiovascular-kidney-metabolic syndrome is a progressive disorder driven by perturbed interorgan crosstalk among adipose, liver, kidney, and heart, leading to multiorgan dysfunction. Capturing the complexity of human cardiovascular-kidney-metabolic pathophysiology using conventional models has been challenging. Multi-organ-on-a-chip platforms offer versatile means study underlying signaling at different stages bolster clinical translation.
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Янв. 15, 2025
ABSTRACT Antibody production is central to protection against new pathogens and cancers, as well certain forms of autoimmunity. Antibodies often originate in the lymph node (LN), specifically at extrafollicular border B cell follicles, where T lymphocytes physically interact drive maturation into antibody-secreting plasmablasts. In vitro models this process are sorely needed predict aspects human immune response. Microphysiological systems (MPSs) offer opportunity approximate lymphoid environment, but so far have focused primarily on memory recall responses antigens previously encountered by donor cells. To date, no 3D culture system has replicated engagement between cells (T—B interaction) that leads antibody when starting with naïve Here, we developed a LN-MPS model early T—B interactions built from primary, encapsulated within collagen-based matrix. Within MPS, exhibited CCL21-dependent chemotaxis chemokinesis predicted. Naïve were successfully skewed chip an follicular helper (pre-Tfh) activated state, respectively, co-culture latter led CD38+ plasmablast dependent IgM. These required differentiation pre-Tfhs, physical cell-cell contact, sensitive ratio which pre-Tfh seeded on-chip. Dependence was greatest 1:5 T:B ratio, while proliferation signal 1:1 ratio. Furthermore, formation established We envision MPS primary lymphocyte physiology will enable mechanistic analyses humoral immunity vitro.
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Апрель 10, 2025
Lymphoid organ function depends on fibroblastic reticular cells (FRCs), the non-hematopoietic mesenchymal stromal that crucially support immune activity in human lymph nodes (LNs). The vitro study of immunology requires physiological LN models, yet inclusion FRCs current models is lacking. Here, we created an organotypic hydrogel model, containing native from tissue and ex vivo cultured autologous FRCs. During a oneweek culture period, enrichment into model benefited viability all cell populations, particularly B cells, promoted presence certain subsets including CD4+ naïve T unswitched (US) memory cells. enhanced production immune-related cytokines chemokines, such as activating factor TNF family (BAFF), CXC motif chemokine ligand 12 (CXCL12), CC 19 (CCL19) interleukin-6 (IL-6). Functionality was assessed through activation by CD3 stimulation or initiation allogenic reaction with different maturation statuses monocyte-derived dendritic (moDCs). Interestingly, expansion restricted FRC-enriched reflecting intrinsic characteristic As such, this highlights influence allows opportunity to further antigen-induced responses, e.g. vaccine immunotherapy testing.
Язык: Английский
Процитировано
0
Reproductive Toxicology, Год журнала: 2025, Номер 135, С. 108951 - 108951
Опубликована: Май 22, 2025
The prevalence of microplastics (MPs)/ nanoplastics (NPs) in the environment has raised significant concerns regarding their potential toxicity, particularly impact on biological systems. These particles, NPs, possess unique properties due to small size and high surface area, enabling them more easily cross barriers accumulate tissues. Among various types plastic materials, polystyrene (PS) is one most studied for its toxicological effects, given widespread use environmental persistence. This narrative review examines current research effects MPs/NPs, immune system, with a focus both development system functional responses. Evidence from vitro vivo studies suggests that MP/NP exposure can disrupt function, including hematopoiesis, cell activation, production inflammatory cytokines. Although highlight cellular toxicity altered behavior, reveal complex outcomes, some findings suggesting organ systems such as spleen intestines, while others indicate minimal or no under environmentally relevant conditions. Here, we aim consolidate summarize evidence topic, key limitations field, identify areas warrant further investigation immunotoxicologists. In addition, emphasize importance using concentrations models better understand risks associated long-term implications health.
Язык: Английский
Процитировано
0
Trends in Molecular Medicine, Год журнала: 2024, Номер unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
2
Bioprinting, Год журнала: 2024, Номер 43, С. e00357 - e00357
Опубликована: Сен. 13, 2024
Язык: Английский
Процитировано
1
Toxicology Letters, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
0
Therapies, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0
Therapies, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Процитировано
0