3‐D Sustained‐Release Culture Carrier Alleviates Rat Intervertebral Disc Degeneration by Targeting STING in Transplanted Skeletal Stem Cells DOI Creative Commons
Liwen Luo, Shiyu Zhang,

Junfeng Gong

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 22, 2025

Abstract The hypoxic and high‐pressure microenvironment of the intervertebral discs poses a major challenge to survival therapeutic efficiency exogenous stem cells. Therefore, improving utilization effect cells delay disc degeneration (IVDD) is great importance. Here, induction studies are conducted in vivo vitro using rat costal cartilage‐derived skeletal (SSCs) find that hypoxia activates cyclic guanosine monophosphate–adenosine monophosphate synthase (cGAS)/stimulator interferon genes (STING) signaling pathway increased reactive oxygen species (ROS) accumulation, triggering ferroptosis SSCs through hypoxia‐inducible factor‐1 alpha‐dependent mitophagy. Progressive preconditioning reduce STING expression ROS inducing differentiation into nucleus pulposus‐like via Wnt pathway. Considering this, 3‐D sustained‐release culture carrier generated by mixing with methacrylated hyaluronic acid polydopamine nanoparticles coated inhibitor C‐176 evaluated its inhibitory on IVDD. This demonstrated inhibit cGAS/STING prevent accumulation continuously releasing C‐176‐coated nanoparticles, thereby reducing ferroptosis, promoting differentiation, ultimately attenuating IVDD, suggesting potential as novel treatment strategy.

Язык: Английский

3‐D Sustained‐Release Culture Carrier Alleviates Rat Intervertebral Disc Degeneration by Targeting STING in Transplanted Skeletal Stem Cells DOI Creative Commons
Liwen Luo, Shiyu Zhang,

Junfeng Gong

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 22, 2025

Abstract The hypoxic and high‐pressure microenvironment of the intervertebral discs poses a major challenge to survival therapeutic efficiency exogenous stem cells. Therefore, improving utilization effect cells delay disc degeneration (IVDD) is great importance. Here, induction studies are conducted in vivo vitro using rat costal cartilage‐derived skeletal (SSCs) find that hypoxia activates cyclic guanosine monophosphate–adenosine monophosphate synthase (cGAS)/stimulator interferon genes (STING) signaling pathway increased reactive oxygen species (ROS) accumulation, triggering ferroptosis SSCs through hypoxia‐inducible factor‐1 alpha‐dependent mitophagy. Progressive preconditioning reduce STING expression ROS inducing differentiation into nucleus pulposus‐like via Wnt pathway. Considering this, 3‐D sustained‐release culture carrier generated by mixing with methacrylated hyaluronic acid polydopamine nanoparticles coated inhibitor C‐176 evaluated its inhibitory on IVDD. This demonstrated inhibit cGAS/STING prevent accumulation continuously releasing C‐176‐coated nanoparticles, thereby reducing ferroptosis, promoting differentiation, ultimately attenuating IVDD, suggesting potential as novel treatment strategy.

Язык: Английский

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