Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 10, 2025
Post-weaning
Diarrhea
(PWD)
is
a
kind
of
physiological
stress
diarrhea
in
Rex
rabbits
after
weaning,
which
can
lead
to
death
severe
cases.
Traditional
Chinese
medicine
(TCM)
has
been
widely
used
animal
due
its
advantages
natural
origin,
diverse
functions,
safety,
reliability,
economy
and
environmental
protection.
Modified
Yupingfeng
Granule
(MYPFG)
an
improved
prescription
based
on
the
famous
traditional
(YPF),
combined
with
other
TCM
obvious
synergistic
additive
activity
order
obtain
excellent
for
PWD.
In
this
study,
120
weaned
were
randomly
allocated
4
treatment
groups,
including
control
(CON),
low
dose
(LD),
medium
(MD),
high
(HD).
Rabbits
fed
diet
or
different
MYPFG
proportions
30
days.
The
study
16S
rRNA
analysis
intestinal
microbiota
cecal
contents
metabolomics
explore
effect
rabbits.
increased
average
daily
gain,
villus
length
crypt
depth
ratio
decreased
feed
meat
ratio,
frequency,
mortality
rate,
(p
<
0.05).
test
found
that
could
change
abundances
Patescibacteria,
Sphingobium,
Ruminococcus,
Oxalobacter.
Metabolomics
may
be
related
regulation
Glycine,
serine
threonine
metabolism,
Arginine
proline
metabolism.
Nicotinate
nicotinamide
regulate
metabolic
pathway
some
amino
acids
alleviate
PWD
Язык: Английский
Substitution of Fishmeal With Soy Protein Concentrate on Growth, Organic Damage, Antioxidant, Transcriptome, and Metabolomics in Macrobrachium rosenbergii
Aquaculture Nutrition,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
The
present
study
investigated
growth
performance,
body
composition,
hepatic
and
intestinal
morphology,
biochemical
indices,
transcriptomic
responses,
metabolomic
profiles
in
giant
freshwater
prawn
(
Macrobrachium
rosenbergii
)
fed
six
kinds
of
soy
protein
concentrate
(SPC)
diets
over
an
8‐week
feeding
trial.
SPC
were
formulated
by
replacing
varying
proportions
fishmeal
(FM)
with
SPC,
the
final
percentage
FM
set
at
350,
280,
210,
140,
70,
0
g/kg,
respectively,
designated
as
F35,
F28,
F21,
F14,
F7,
F0
diets.
Results
showed
that
weight
(FBW),
gain,
specific
rate
(SGR)
F35
greater
than
those
F7
F0.
crude
lipid
content
was
higher
F28.
Notably,
increasing
substitution
levels
disrupted
hepatopancreas
structural
degradation
becoming
more
pronounced
beyond
40%
replacement
(F21).
Compared
to
group,
significantly
decreased
activity
glutathione
peroxidase
(GSH‐PX),
increases
malondialdehyde
(MDA)
nitric
oxide
(NO)
hemolymph.
Based
on
transcriptomics,
two
differentially
expressed
genes
(DEGs)
LOC136825138
LOC136856310
consistently
observed
across
all
groups.
metabolomics
indicated
77
metabolites
(DEMs)
treatments.
A
negative
correlation
between
eicosapentaenoic
acid
(EPA),
arachidonoyl
dopamine,
8Z,11Z‐eicosadienoic
acid,
vitamin
E
nicotinate.
comprehensive
analysis
both
data
sets
revealed
substantial
perturbations
associated
“alpha‐linolenic
metabolism”
“glycerophospholipid
metabolism”.
In
conclusion,
elevated
dietary
had
detrimental
effects
health,
antioxidant
capacity,
immune
function
M.
.
level
for
could
be
reduced
140
g/kg
using
a
sole
substitute,
inclusion
211
diet.
Язык: Английский
Seaweed (Pterocladia capillacea) nanoparticles improves growth performances, digestive enzymes, antioxidant activities, innate immunity, and related-immunity gene expressions of Litopenaeus vannamei
Frontiers in Marine Science,
Год журнала:
2025,
Номер
12
Опубликована: Июнь 3, 2025
This
work
evaluated
the
effects
of
dietary
supplementation
with
seaweed,
Pterocladia
capillacea
,
nanoparticles
(SN)
on
growth,
whole-body
composition,
digestive
enzyme
activities,
feed
efficiency,
immunological
response,
antioxidant
activity,
and
gene
expression
whiteleg
shrimp,
Litopenaeus
vannamei
.
The
SN
form
was
conducted
using
a
Planetary
Ball
Mill
PM
400.
particle
size
verified
through
Dynamic
Light
Scattering
(DLS)
analysis.
DLS
showed
that
mean
sizes
were
between
151
nm
(13.6%)
835
(64%).
Throughout
60
day
experimental
trial,
postlarvae
(PLs)
L.
subjected
to
one
following
five
feeding
groups.
first
group
is
commercially
available
shrimp
as
basal
diet
without
any
seaweed
supplementation,
functioning
negative
control
(C
0%
).
A
second
received
commercial
supplemented
2%
(20
g/kg)
dried
powder
(SP
),
positive
control.
remaining
three
groups
fed
diets
at
concentrations
0.5%
(SN
1%
respectively.
750
postlarval
(0.053
g/PL)
allocated
Each
consisted
150
PLs
(triplicate).
their
corresponding
regime
times
10%
body
weight.
results
revealed
that,
compared
those
)
or
controls,
increasing
levels
SN,
especially
growth
(FW,
WG,
SGR),
enzymes
(amylase
lipase
activities),
carcass
composition
(protein
lipid
contents),
nutrient
efficiency
(FI,
PI,
FER,
FCR,
PER),
activities
(SOD
CAT),
innate
immunity
(LYS
MDA),
related-immunity
expressions
(
p53
significantly
improved.
In
conclusion,
these
findings
concluded
applying
nanotechnology
tools
enhances
additives
maximizes
health,
overall
production.
Further
research
required
understand
explain
how
affect
shrimp’s
physiological
state
upgrade
some
immune-related
expressions.
Язык: Английский