Advances in Sampling and Analytical Techniques for Single‐Cell Metabolomics: Exploring Cellular Heterogeneity DOI

Xinxin Shen,

Fangyuan Zhang, Chunping Tang

и другие.

Rapid Communications in Mass Spectrometry, Год журнала: 2025, Номер 39(13)

Опубликована: Апрель 13, 2025

ABSTRACT Single‐cell metabolomics is an emerging and powerful technology that uncovers intercellular heterogeneity reveals microenvironmental dynamics in both physiological pathological conditions. This enables detailed observations of cellular interactions, providing valuable insights into processes such as aging, immune responses, disease development. Despite significant advances, the need for discussions on sampling analytical methods single‐cell continues to grow, with increasing focus selecting most suitable techniques diverse research objectives. review addresses these challenges by exploring key strategies used metabolomics. We three main approaches: capture isolation specific cell types, precise aspiration individual cells, situ mass spectrometry imaging. These are critically assessed highlight achieving accurate metabolite detection at level across applications.

Язык: Английский

Advances in Sampling and Analytical Techniques for Single‐Cell Metabolomics: Exploring Cellular Heterogeneity DOI

Xinxin Shen,

Fangyuan Zhang, Chunping Tang

и другие.

Rapid Communications in Mass Spectrometry, Год журнала: 2025, Номер 39(13)

Опубликована: Апрель 13, 2025

ABSTRACT Single‐cell metabolomics is an emerging and powerful technology that uncovers intercellular heterogeneity reveals microenvironmental dynamics in both physiological pathological conditions. This enables detailed observations of cellular interactions, providing valuable insights into processes such as aging, immune responses, disease development. Despite significant advances, the need for discussions on sampling analytical methods single‐cell continues to grow, with increasing focus selecting most suitable techniques diverse research objectives. review addresses these challenges by exploring key strategies used metabolomics. We three main approaches: capture isolation specific cell types, precise aspiration individual cells, situ mass spectrometry imaging. These are critically assessed highlight achieving accurate metabolite detection at level across applications.

Язык: Английский

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