Production and characterization of novel antioxidant peptides from mulberry leaf ferment using B. subtilis H4 and B. amyloliquefaciens LFB112 DOI Creative Commons
Zaheer Abbas, Jing Zhang,

Yucui Tong

и другие.

Food Chemistry, Год журнала: 2025, Номер 482, С. 144022 - 144022

Опубликована: Апрель 6, 2025

The objective of this study was to isolate and characterize antioxidant peptides from mulberry leaves fermented with Bacillus subtilis H4 amyloliquefaciens LFB112. results indicated that fraction F4 (<1 kDa) exhibited superior DPPH activity compared the F1 (>10 kDa), F2 (3-10 F3 (1-3 fractions, cytoprotective effect against lipopolysaccharides (LPS)-induced oxidative stress in RAW264.7 cells. Three novel peptides, FRFDP, RFGG, GPPLAFGGGP, were identified leaf ferment, docking showed these could form stable carbon (covalent) hydrogen bonds active sites Keap1, thus regulating Keap1-Nrf2 pathway by blocking Nrf2 binding on Keap1. These significantly upregulated mRNA expression Nrf2, HO-1, NQO1, providing indirect evidence their potential enhance cellular defense via possible activation pathway. Furthermore, good ABTS scavenging activities, indicating as peptides. This offers valuable insights into integration developing ferment a functional food use feed additive.

Язык: Английский

Production and characterization of novel antioxidant peptides from mulberry leaf ferment using B. subtilis H4 and B. amyloliquefaciens LFB112 DOI Creative Commons
Zaheer Abbas, Jing Zhang,

Yucui Tong

и другие.

Food Chemistry, Год журнала: 2025, Номер 482, С. 144022 - 144022

Опубликована: Апрель 6, 2025

The objective of this study was to isolate and characterize antioxidant peptides from mulberry leaves fermented with Bacillus subtilis H4 amyloliquefaciens LFB112. results indicated that fraction F4 (<1 kDa) exhibited superior DPPH activity compared the F1 (>10 kDa), F2 (3-10 F3 (1-3 fractions, cytoprotective effect against lipopolysaccharides (LPS)-induced oxidative stress in RAW264.7 cells. Three novel peptides, FRFDP, RFGG, GPPLAFGGGP, were identified leaf ferment, docking showed these could form stable carbon (covalent) hydrogen bonds active sites Keap1, thus regulating Keap1-Nrf2 pathway by blocking Nrf2 binding on Keap1. These significantly upregulated mRNA expression Nrf2, HO-1, NQO1, providing indirect evidence their potential enhance cellular defense via possible activation pathway. Furthermore, good ABTS scavenging activities, indicating as peptides. This offers valuable insights into integration developing ferment a functional food use feed additive.

Язык: Английский

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