Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Май 29, 2025

Objective ME1 catalyzes the conversion of malic acid into acetic acid, thereby linking glucose metabolism to citric cycle. In recent years, role in various tumors has only been superficially explored. Therefore, our objective is analyze potential functions pan-cancer, with a particular focus on its ovarian cancer. Methods We analyzed expression levels both normal and tumor tissues across cancer types. CBIOPORTAL was utilized assess mutation frequency specific sites ME1. Additionally, we examined correlation between several factors, including methylation status, burden (TMB), microsatellite instability (MSI), immune regulator genes, checkpoints, microenvironment scores, functional enrichment, single-cell analysis, drug sensitivity. The Estimate Algorithm assessed immunochemical microenvironment. Small interfering RNA chronic viruses were downregulate upregulate two cell lines, respectively, conduct experiments proliferation migration. Results Our results revealed that exhibited disorders tumors, predominant form genetic identified being missense mutation. Among analyzed, demonstrated significant levels, TMB, MSI, immunomodulatory regulatory infiltration. Functional enrichment analysis indicated associated metabolic regulation, macrophage responses, antioxidant defense mechanisms, elevated may be more favorable response immunotherapy, suggesting applications guiding immunotherapeutic strategies. vitro research knockdown inhibited migration cells. Conversely, overexpression appeared promote Conclusions ME1, metabolic-related factor, serve as biomarker for progression infiltration, particularly It signify reprogramming supplies energy offering valuable insights development personalized therapies.

Язык: Английский