Ecogenomics reveals distinctive viral-bacterial communities in the surface microlayer of a natural surface slick DOI Creative Commons
Janina Rahlff, Matthias Wietz, Helge‐Ansgar Giebel

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Фев. 24, 2023

Abstract Visible surface films, termed slicks, can extensively cover the sea surface, particularly in coastal regions. The sea-surface microlayer (SML), upper 1-mm at air-water interface slicks (slick SML) harbors a distinctive bacterial community, but little is known about SML viruses. Using flow cytometry, metagenomics, and cultivation, we investigated viruses community from brackish slick comparison to non-slick as well seawater below (SSW). We conducted size-fractionated filtration of all samples distinguish viral attachment hosts particles. contained higher abundances virus-like particles, prokaryotic cells, dissolved organic carbon compared SSW. 428 operational taxonomic units (vOTUs), 426 predicted lytic, distinctly differed across size fractions distinctness was underlined by specific metabolic profiles metagenome assembled genomes isolates, which revealed prevalence motility genes diversity CAZymes SML. Despite overall lower diversity, several vOTUs were enriched over Nine only found six them targeted SML-specific CRISPR spacers likely originating Gammaproteobacteria. Moreover, isolation three previously unknown lytic phages for Alishewanella sp. Pseudoalteromonas tunicata , representing abundant actively replicating bacteria, suggests that activity contribute biogeochemical cycling ecosystems.

Язык: Английский

Layered aquatic microenvironments control fluctuations generated by active carpets DOI Creative Commons
Felipe A. Barros, Hugo N. Ulloa, Gabriel Aguayo

и другие.

Physical Review Research, Год журнала: 2025, Номер 7(1)

Опубликована: Фев. 12, 2025

In Earth's aquatic environments and the human body, microbial swimmers often accumulate at interfaces within layered systems, forming colonies known as . These bioactive layers enhance mass transport diffusion in fluid media. Here we study hydrodynamic behavior induced by confined semi-infinite layers, such one found sea surface microlayer. By deriving analytical expressions performing numerical simulations, explore how geometrical viscous confinement (layer thickness viscosity ratio) influence fluctuations passive tracer dynamics. Our findings reveal anisotropic distributions of fluctuations, characterized three distinct regions: near fluid-fluid interface (Region I), vertical dominate; an intermediate region II), become isotropic; free III), horizontal prevail. The results also demonstrate emergence coherent vortical structures highly with roll-like patterns governed layer sharpness transitions. insights provided this work have implications for understanding biogenic flow processes natural engineered environments, offering potential applications areas ecology, biofilm management, microfluidic technologies. Published American Physical Society 2025

Язык: Английский

Процитировано

0
Selective Ocean–Atmosphere Bacterial Flux Through the Pacific Sea Surface Microlayer DOI Creative Commons

Ariel C. Tastassa,

Yael Dubowski,

Or Argaman Meirovich

и другие.

ACS ES&T Air, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Язык: Английский

Процитировано

0
Ecogenomics and cultivation reveal distinctive viral-bacterial communities in the surface microlayer of a Baltic Sea slick DOI Creative Commons
Janina Rahlff, Matthias Wietz, Helge‐Ansgar Giebel

и другие.

ISME Communications, Год журнала: 2023, Номер 3(1)

Опубликована: Сен. 18, 2023

Visible surface films, termed slicks, can extensively cover freshwater and marine ecosystems, with coastal regions being particularly susceptible to their presence. The sea-surface microlayer (SML), the upper 1-mm at air-water interface in slicks (herein slick SML) harbors a distinctive bacterial community, but generally little is known about SML viruses. Using flow cytometry, metagenomics, cultivation, we characterized viruses bacteria brackish comparison non-slick as well seawater below areas (subsurface water = SSW). Size-fractionated filtration of all samples distinguished viral attachment hosts particles. contained higher abundances virus-like particles, prokaryotic cells, dissolved organic carbon compared SSW. community 428 operational taxonomic units (vOTUs), 426 predicted lytic, distinctly differed across size fractions Specific metabolic profiles metagenome-assembled genomes isolates included prevalence genes encoding motility carbohydrate-active enzymes (CAZymes). Several vOTUs were enriched SML, many virus variants associated Nine only found six them targeted by SML-specific clustered-regularly interspaced short palindromic repeats (CRISPR) spacers likely originating from Gammaproteobacteria. Moreover, isolation three previously unknown lytic phages for Alishewanella sp. Pseudoalteromonas tunicata, abundant actively replicating bacteria, suggests that activity contributes biogeochemical cycling ecosystems.

Язык: Английский

Процитировано

5
Seasonal dynamics response mechanism of benthic microbial community to artificial reef habitats DOI
Zhansheng Guo, Lu Wang, Minpeng Song

и другие.

Environmental Research, Год журнала: 2023, Номер 243, С. 117867 - 117867

Опубликована: Дек. 7, 2023

Язык: Английский

Процитировано

4
Particle separation mechanisms in suspension-feeding fishes: key questions and future directions DOI Creative Commons
S. Laurie Sanderson

Frontiers in Marine Science, Год журнала: 2024, Номер 11

Опубликована: Апрель 2, 2024

Key unresolved questions about particle separation mechanisms in suspension-feeding fishes are identified and discussed, focusing on areas with the potential for substantial future discovery. The published hypotheses that explored have broad applicability to biological filtration bioinspired improvements commercial industrial crossflow microfiltration processes microfluidics. As first synthesis of primary literature marine, estuarine, freshwater fishes, goals enable comparisons invertebrate processes, stimulate theoretical empirical studies, further development biomimetic physical computational fluid dynamics models. Of eight six been proposed within past twenty years (inertial lift shear-induced migration, reduction effective gap size by vortices, cross-step filtration, vortical flow along outer faces gill raker plates, ricochet lateral displacement). pace discovery is anticipated continue accelerating. Multidisciplinary collaboration integration among biologists engineers (including chemical, mechanical, biomedical, engineering) will result new perspectives identify patterns unifying across breadth fish taxa, morphology, function.

Язык: Английский

Процитировано

1
Onset of cabbeling instabilities in superconfined two-fluid systems DOI
Julio Leyrer Hernández, Hugo N. Ulloa, Jaime H. Ortega

и другие.

Physics of Fluids, Год журнала: 2024, Номер 36(12)

Опубликована: Дек. 1, 2024

Convective-driven mixing in permeable subsurface environments is relevant engineering and natural systems. This process occurs groundwater remediation, oil recovery, CO2 sequestration, hydrothermal environments. When two fluids come into contact superconfined geometries like open fractures rocks, complex molecular dynamics can develop at the fluid–fluid interface, creating a denser mixture leading to cabbeling instabilities that propel solutal convection. Previous studies systems have used models based on unstable density distributions—generating Rayleigh–Taylor instabilities—and analog fluid mixtures characterized by nonlinear equations of state—resulting dynamics—yet often neglecting interfacial tension effects, which also miscible study incorporates Korteweg tensor Hele–Shaw model better understand combined influence geometry confinement onset two-fluid Through direct numerical simulations, we investigate system's stability, revealing onset, critical time tc, exhibits relationship with nondimensional parameters—the Rayleigh number Ra, anisotropy ratio ϵ, Ko. crystallized single scaling law tc=F(Ra,ϵ,Ko). Our findings indicate effective exert stabilizing effect during initial stages convection, stressing necessity for further exploration its

Язык: Английский

Процитировано

0
Ecogenomics reveals distinctive viral-bacterial communities in the surface microlayer of a natural surface slick DOI Creative Commons
Janina Rahlff, Matthias Wietz, Helge‐Ansgar Giebel

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Фев. 24, 2023

Abstract Visible surface films, termed slicks, can extensively cover the sea surface, particularly in coastal regions. The sea-surface microlayer (SML), upper 1-mm at air-water interface slicks (slick SML) harbors a distinctive bacterial community, but little is known about SML viruses. Using flow cytometry, metagenomics, and cultivation, we investigated viruses community from brackish slick comparison to non-slick as well seawater below (SSW). We conducted size-fractionated filtration of all samples distinguish viral attachment hosts particles. contained higher abundances virus-like particles, prokaryotic cells, dissolved organic carbon compared SSW. 428 operational taxonomic units (vOTUs), 426 predicted lytic, distinctly differed across size fractions distinctness was underlined by specific metabolic profiles metagenome assembled genomes isolates, which revealed prevalence motility genes diversity CAZymes SML. Despite overall lower diversity, several vOTUs were enriched over Nine only found six them targeted SML-specific CRISPR spacers likely originating Gammaproteobacteria. Moreover, isolation three previously unknown lytic phages for Alishewanella sp. Pseudoalteromonas tunicata , representing abundant actively replicating bacteria, suggests that activity contribute biogeochemical cycling ecosystems.

Язык: Английский

Процитировано

1