Temporal dynamics of the bacterial community structure and functions associated with marine sponges collected off Karah Island, Terengganu, Malaysia

The Microbe, Год журнала: 2024, Номер 5, С. 100202 - 100202

Опубликована: Ноя. 10, 2024

Язык: Английский

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A Chemo-Ecological Investigation of Dendrilla antarctica Topsent, 1905: Identification of Deceptionin and the Effects of Heat Stress and Predation Pressure on Its Terpene Profiles

Marine Drugs, Год журнала: 2023, Номер 21(9), С. 499 - 499

Опубликована: Сен. 19, 2023

Marine sponges usually host a wide array of secondary metabolites that play crucial roles in their biological interactions. The factors influence the intraspecific variability metabolic profile organisms, production or ecological function remain generally unknown. Understanding this may help predict changes relationships due to environmental variations as consequence climate change. sponge Dendrilla antarctica is common shallow rocky bottoms Antarctic Peninsula and known produce diterpenes are supposed have defensive roles. Here we used GC-MS determine major two populations D. from islands, Livingston Deception Island (South Shetland Islands). To assess potential effect heat stress, exposed aquaria control temperature (similar local), stress (five degrees higher) extreme (ten higher). test for defence induction by predation pressure, sea star Odontaster validus amphipod Cheirimedon femoratus. Seven were isolated identified samples. While six them already reported literature, one new aplysulphurane derivative was more abundant samples Island, so named it deceptionin (7). separated PCA space according island collection, with 9,11-dihydrogracilin A (1) being Livingston, (7) Deception. We found slight on diterpene profiles antarctica, tetrahydroaplysulphurin-1 (6) gracilane norditerpene 2 group stress. Predation pressure did not seem metabolite production. Further research bioactivity metabolites, responses will better understand functioning fate benthos.

Язык: Английский

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Dynamics, diversity, and roles of bacterial transmission modes during the first asexual life stages of the freshwater sponge Spongilla lacustris

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Фев. 23, 2024

Background: Sponges-associated bacteria play important roles in the physiology of their host, whose recruitment processes are crucial to maintain symbiotic associations. However, acquisition bacterial communities within freshwater sponges is still unexplored. Spongilla lacustris a model sponge widely distributed European rivers and lakes, producing dormant cysts (named gemmules) for asexual reproduction, before winter. Through an in vitroexperiment, this study aims describe dynamics transmission modes following hatching these gemmules. Results: An overall change β-diversity was observed through ontology new juvenile sponges. These temporal differences were potentially linked first osculum development canal system, then, increasing colonization Chlorella-like photosymbionts. Gemmules with sterilized surface found have more dispersed less diverse microbiome, revealing importance gemmule epibacteria whole holobiont stability. suggested be vertically transmitted from maternal tissues surface. Vertical incorporation inside gemmule, also as dominant mode, especially nitrogen fixers Terasakiellaceae. Finally, we showed that almost no ASVs shared between free-living community juveniles, suggesting horizontal unlikely happen during stages development. filtered probably used source nutrients, allowing enrichment copiotrophic already present its microbiome. Conclusions: This brings insight better understanding microbiome We epibacterial on gemmules stability, demonstrated near absence stages.

Язык: Английский

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Climate change impacts on tropical sponges and associated microbial communities

Опубликована: Апрель 28, 2024

<p><strong>Climate change is causing rapid changes in reef structure, biodiversity, and function as a response to ocean warming acidification. The negative impacts of climate on corals are well-documented, but most sponges predicted tolerate conditions projected for 2100, making them viable option stable alternative states. Sponges maintain an intimate relationship with microbial communities, whose general stability given sponge species makes suitable indicators host responses stressful environmental such overall aim this thesis examine the their associated focus community composition abundance protein expression.</strong></p><p>The first data chapter examines results from reciprocal transplantation coral Coelocarteria singaporensis Stylissa cf. flabelliformis between control site adjacent CO2 vent Papua New Guinea explore how microbiome responds acidification situ. Microbial communities C. singaporensis, which differed initially sites, did not shift towards characteristic or microbiomes. S. flabelliformis, were respond specifically collectively exhibited significant over time, relative increase Thaumarchaeota decrease Proteobacteria all treatment groups. lack upon suggests that flexibility, at least adult life-history stage, does necessarily underpin survival under acidification.</p><p>The second compares symbiotic tropical after eight-week exposure nine different treatments three temperatures (28.5 °C, 30 31.5 °C) pH levels (8.1, 7.8, 7.6) based ambient two IPCC predictions future (RCP6.0 RCP8.5). Bacterial significantly temperature treatments, pH, highest showing increased abundances Bacteroidia Clostridia (commonly disease thermal stress), decreased Nitrospira (associated nitrogen cycling). Similarly, archaeal across temperature, Nitrososphaeria ammonia oxidation). Symbiotic eukaryotes compared using reference databases only 8.1 difference also highlighted requirement more thorough databases. Overall, there was clear groups, indicating stress potential hindrance nutrient cycling.</p><p>The third aims determine impact spatial arrangement sponge-associated microbes droplet digital PCR (ddPCR) fluorescence situ hybridization (FISH). Total bacterial unexpectedly high replicates notable algal growth tissue necrosis. Archaea decreasing increasing no due pH. FISH largely unsuccessful, primarily autofluorescence very low concentration archaea (as shown ddPCR). Regardless, shifts reduced health show signs functional implications.</p><p>The fourth uses proteomic analysis expression High-temperature proteins heat shock oxidative stress, actin-related processes, smooth muscle contraction comparison low-temperature treatments. high- low-pH resulted annotated biological processes. Oxidation higher 7.6 while lower 7.6. important functions reduction indicate flabelliformis.</p><p>Overall, expected be resilient than calcifying organisms corals. Although produced little holobiont, exhibits threshold approximately °C. At implications roles sponges.</p>

Язык: Английский

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Putative past, present, and future spatial distributions of deep-sea coral and sponge microbiomes revealed by predictive models

ISME Communications, Год журнала: 2024, Номер 4(1)

Опубликована: Янв. 1, 2024

Abstract Knowledge of spatial distribution patterns biodiversity is key to evaluate and ensure ocean integrity resilience. Especially for the deep ocean, where in situ monitoring requires sophisticated instruments considerable financial investments, modeling approaches are crucial move from scattered data points predictive continuous maps. Those commonly run on macrobial level, but spatio-temporal predictions host-associated microbiomes not being targeted. This especially problematic as previous research has highlighted that microbes may display perfectly correlated only with host biogeographies, also other factors, such prevailing environmental conditions. We here establish a new simulation approach present predicted deep-sea sponge coral microbiomes, making use combination data, microbiome data. allows scales currently covered by classical sampling at sea. In summary, our presented allow (i) identification microbial hotspots past, present, future, (ii) trait-based link biodiversity, (iii) shifts community composition (key taxa) across gradients shifting

Язык: Английский

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Phage-induced disturbance of a marine sponge microbiome

Environmental Microbiome, Год журнала: 2024, Номер 19(1)

Опубликована: Ноя. 26, 2024

Abstract Background Bacteriophages are known modulators of community composition and activity in environmental host-associated microbiomes. However, the impact single phages have on bacterial dynamics under viral predation, extent duration their effect, not completely understood. In this study, we combine morphological genomic characterization a novel marine phage, isolated from Baltic sponge Halichondria panicea , report first attempts controlled phage-manipulation natural sponge-associated Results We used culture-based culture-independent (16S rRNA gene amplicon sequencing) methods to investigate microbiomes with without addition phages. Upon application Maribacter specialist phage Panino conditions, were able detect community-wide shifts microbiome load after 72 h. While became more dissimilar over time presence phages, species evenness richness maintained. exposure, observed loss several low-abundance constituent taxa resident microbiota, while other originally underrepresented increased. Virulent likely induce disturbances, evident changes total microbial profile by specific elimination taxa, which leads an increase abundance opportunistic such as genera Vibrio Pseudoalteromonas Photobacterium . Conclusions Our findings suggest that diversity and, extension, its resilience depend maintenance members, irrespective abundance. Phage-induced disturbances can significantly alter structure promoting growth bacteria like shifting dysbiotic state. These insights highlight role bacteriophages shaping underscore potential for managing environments.

Язык: Английский

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