Clownfish larvae exhibit faster growth, higher metabolic rates and altered gene expression under future ocean warming

The Science of The Total Environment, Год журнала: 2023, Номер 873, С. 162296 - 162296

Опубликована: Фев. 17, 2023

Increasing ocean temperatures have been demonstrated to a range of negative impacts on coral reef fishes. However, despite wealth studies juvenile/adult fish, how early developmental stages respond warming are limited. As overall population persistence is influenced by the development life stages, detailed larval responses essential. Here, in an aquaria-based study we investigate associated with future and present-day marine heatwaves (+3 °C) impact growth, metabolic rate, transcriptome 6 discrete clownfish larvae (Amphiprion ocellaris). A total clutches were assessed, 897 imaged, 262 undergoing testing 108 subject sequencing. Our results show that reared at +3 °C grow develop significantly faster exhibit higher rates than those control conditions. Finally, highlight molecular mechanisms underpinning response from different temperatures, genes metabolism, neurotransmission, heat stress epigenetic reprogramming differentially expressed °C. Overall, these indicate could be altered under warming, gene expression all affected. Such changes may lead dispersal, settlement time increased energetic costs.

Язык: Английский

---

Parental thermal environment controls the offspring phenotype in Brook charr (Salvelinus fontinalis): insights from a transcriptomic study

G3 Genes Genomes Genetics, Год журнала: 2024, Номер 14(5)

Опубликована: Март 13, 2024

Abstract Brook charr is a cold-water species which highly sensitive to increased water temperatures, such as those associated with climate change. Environmental variation can potentially induce phenotypic changes that are inherited across generations, for instance, via epigenetic mechanisms. Here, we tested whether parental thermal regimes (intergenerational plasticity) and offspring-rearing temperatures (within-generational modify the brain transcriptome of progeny (fry stage). Parents were exposed either cold or warm during final gonad maturation their reared at 5 8 °C first stages development. Illumina Novaseq6000 was used sequence yolk sac resorption stage. The number differentially expressed genes very low when comparing fry different (79 genes). In contrast, 9,050 significantly between issued from parents temperatures. There significant downregulation processes related neural synaptic activity in originating group vs one. We also observed upregulation DNA methylation most salient compensation warming, metabolism, cellular response stress, adaptive immunity.

Язык: Английский

---

Transcriptome analyses reveal differences in the response to temperature in Florida and Northern largemouth bass (Micropterus spp.) during early life stages

PLoS ONE, Год журнала: 2025, Номер 20(2), С. e0317563 - e0317563

Опубликована: Фев. 18, 2025

Temperature is one of the most relevant factors influencing development aquatic species, making it a key parameter to consider for aquaculture. Largemouth bass (LMB; Micropterus spp .) are highly human consumption and sport fishing, representing North America’s important freshwater fisheries. Yet, questions remain on how LMB raised in recirculating aquaculture systems (RAS) respond different temperatures. The main objective this study was determine impact thermal rearing conditions (21°C, 24°C, 27°C) gene expression Florida Northern larvae at 8- 28-days post hatch (DPH). Using de novo transcriptomes as reference, our results suggest that differences were mostly associated with temperature, while controlled by temperature developmental stage. In general, both lineages showed activation molecular pathways growth, such muscle, nervous system, vascular system. There signatures stress warming well, including immune function, apoptosis, regulation inflammation, heat shock proteins. large between temperatures stages, much larger 28 DPH, specifically individuals reared 27°C. from line previous phenotypic studies indicated faster growth warmer better performance RAS. Overall, exemplifies controlling during critical early life stages can be essential guarantee success commercial hatchery production techniques.

Язык: Английский

---

Placing human gene families into their evolutionary context

Human Genomics, Год журнала: 2022, Номер 16(1)

Опубликована: Ноя. 11, 2022

Abstract Following the draft sequence of first human genome over 20 years ago, we have achieved unprecedented insights into rules governing its evolution, often with direct translational relevance to specific diseases. However, staggering complexity has also challenged development a more comprehensive understanding biology. In this context, interspecific genomic studies between humans and other animals played critical role in our efforts decode gene families. review, focus on how rapid surge sequencing both model non-model organisms now provides broader comparative framework poised empower novel discoveries. We begin general overview approaches are essential for family evolution genome, followed by discussion analyses expression. show homology can provide genes families associated immune response, cancer biology, vision, chemosensation, metabolism, revealing similarity processes among distant species. then explain methodological tools that advances limitations common approaches. conclude these investigations position us gain fundamental living general. hope review catalyzes additional excitement research emerging field genomics, while aiding placement existentially evolutionary context.

Язык: Английский

---

Tissue-specific transcriptional response of post-larval clownfish to ocean warming

The Science of The Total Environment, Год журнала: 2023, Номер 908, С. 168221 - 168221

Опубликована: Ноя. 2, 2023

Anthropogenically driven climate change is predicted to increase average sea surface temperatures, as well the frequency and intensity of marine heatwaves in future. This increasing temperature have a range negative physiological impacts on multiple life-stages coral reef fish. Nevertheless, studies early-life stages remain limited, tissue-specific transcriptomic post-larval fish are yet be conducted. Here, an aquaria-based study we investigate (brain, liver, muscle, digestive tract) response (20 dph) Amphiprion ocellaris temperatures associated with future (+3 °C). Additionally, utilized metatranscriptomic sequencing how microbiome tract changes at +3 °C. Our results show that transcriptional elevated highly tissue-specific, number differentially expressed genes (DEGs) gene functions varied amongst brain (102), liver (1785), (380), muscle (447). All tissues displayed DEGs thermal stress, 23 heat-shock protein were upregulated all tissues. indicate clownfish may experience fibrosis-like symptoms °C extracellular matrix structure, oxidative inflammation, glucose transport, metabolism upregulated. We also observe shift community Vibrio sp. replace Escherichia coli dominant bacteria. coupled dysregulation various involved immune tract. Overall, this highlights will display responses increases temperature, many potentially harmful pathways activated

Язык: Английский

---

Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes)

Frontiers in Marine Science, Год журнала: 2022, Номер 9

Опубликована: Ноя. 29, 2022

The tiger puffer ( Takifugu rubripes ) is an important economic fish species in northern China. However, it a warm-temperature species, and low winter temperatures can result high mortality aquaculture. Understanding the mechanisms of cold resistance puffers will thus provide critical information to help cope with cold. In this study, we performed transcriptome analysis livers from kept at different (18°C, 13°C, 8°C) identify key pathways genes involved response low-temperature stress. We also detected serum levels proteases, arginine, proline obtain further on adaption. Totals 51, 942, 195 differentially expressed were identified 18°C vs 8°C, 13°C 8°C groups, respectively. Pathway showed that significantly enriched mainly related digestion, metabolism, environmental adaptation. Most digestion metabolism down-regulated, while most adaptation up-regulated. Serum proteases lower groups (13°C compared control group (18°C), arginine higher other two groups. These results suggest temperature caused digestive metabolic disorders, as well adaptive changes puffers. On premise, found some up-regulated pancreatic secretion pathway, circadian rhythm pathway played roles survival, growth, development under accumulation maintain circulation resist closely which change plays positive role temperature. study new insights theoretical basis for tolerance

Язык: Английский

---

Transposable Element Tissue-Specific Response to Temperature Stress in the Stenothermal Fish Puntius tetrazona

Animals, Год журнала: 2022, Номер 13(1), С. 1 - 1

Опубликована: Дек. 20, 2022

Ray-finned fish represent a very interesting group of vertebrates comprising variety organisms living in different aquatic environments worldwide. In the case stenothermal fish, thermal fluctuations are poorly tolerated, thus ambient temperature represents critical factor. this paper, we considered tiger barb Puntius tetrazona, freshwater belonging to family Cyprinidae, at 21–28 °C. We analyzed available RNA-Seq data obtained from specimens exposed 27 °C and 13 investigate transcriptional activity transposable elements (TEs) genes encoding for proteins involved their silencing brain, gill, liver. TEs one tools generating genetic variability that underlies biological evolution, useful adapt environmental changes. Our findings highlighted response three tissues. While brain no variation TE was observed, remarkable increase recorded Moreover, analysis such as heterochromatin formation, NuRD complex, RISC complex (e.g., AGO GW182 proteins) hepatic tissue. Overall, our suggested tissue is target organ kind stress, since activation might regulate expression stress-induced genes, leading better organism Therefore, view corroborates once again idea potential role rapid adaptation, hence representing promising molecular tool species resilience.

Язык: Английский

---

Molecular plasticity to ocean warming and habitat loss in a coral reef fish

Journal of Heredity, Год журнала: 2024, Номер unknown

Опубликована: Апрель 23, 2024

Abstract Sea surface temperatures are rising at unprecedented rates, leading to a progressive degradation of complex habitats formed by coral reefs. In parallel, acute thermal stress can lead physiological challenges for ectotherms that inhabit reefs, including fishes. Warming and habitat simplification could push marine fishes beyond their limits in the near future. Specifically, questions remain on how warming structure influence brains Here we evaluated loss acting independently synergistically as stressors damselfish Western Atlantic, Abudefduf saxatilis. For this experiment, 40 individuals were exposed different combinations temperature (27 °C or 31 °C) complexity (complex vs. simple) 10 days, changes brain gene expression oxidative liver muscle evaluated. The results indicate resulted increased damage (P = 0.007) genes associated with neurotransmission, immune function, tissue repair. Individuals from simplified showed higher numbers differentially expressed synaptic plasticity spatial memory. addition, reference transcriptome A. saxatilis is presented here first time, serving resource future molecular studies. This project enhances our understanding responding combination reef while elucidating plastic mechanisms will enable generalists persist changing world.

Язык: Английский

---

Parental thermal conditions affect the brain activity response to alarm cue in larval zebrafish

PeerJ, Год журнала: 2024, Номер 12, С. e18241 - e18241

Опубликована: Окт. 10, 2024

Temperature is a crucial factor affecting the physiology of ectothermic animals, but exposure to elevated temperature during specific life stages and across generations may confer fish resilience through phenotypic plasticity. In this study, we investigate effects developmental parental on brain activity response an olfactory cue in larval zebrafish, Danio rerio . We exposed parents reproduction their offspring development control (28 °C) or (30 observed telencephalon alarm using live calcium imaging. Parental decreased time till maximum regardless offspring’s temperature, revealing that thermal conditions can affect excitability neural circuitry. Furthermore, duration was affected by interaction between conditions, where longer seen when either were temperature. Conversely, found shorter same as parents, both This could represent anticipatory effect influencing match environment, early occurring within susceptible short window post-fertilization. Overall, our results suggest warming alter processes involved transmission show aid preparation respond stimuli environment.

Язык: Английский

---

Matching maternal and paternal experiences underpin molecular thermal acclimation

Molecular Ecology, Год журнала: 2024, Номер unknown

Опубликована: Март 23, 2024

The environment experienced by one generation has the potential to affect subsequent through non-genetic inheritance of parental effects. Since both mothers and fathers can influence their offspring, questions arise regarding how maternal, paternal offspring experiences integrate into resulting phenotype. We aimed disentangle maternal contributions transgenerational thermal acclimation in a reef fish, Acanthochromis polyacanthus, exposing two generations elevated temperature (+1.5°C) fully factorial design analysing F2 hepatic gene expression. Paternal effects showed not only common but also parent-specific components, with father having largest shaping offspring's transcriptomic profile. Fathers contributed transcriptional response warming transfer epigenetically controlled stress-response mechanisms while influenced increased expression associated lipid metabolism regulation. However, key was matching parents. When parents were exposed same condition, genes related structural RNA production regulation, whereas environmental mismatch resulted maladaptive condition transfer, revealed translation suppression endoplasmic reticulum stress. Interestingly, own experience had smallest on transcription profiles. Taken together, our results show complex nature interplay among paternal, cue integration, reveal that ocean might depend importantly congruent experiences.

Язык: Английский

---