Genomic evolution of SARS-CoV-2 in Morocco: Insights from whole genome sequences collected from 2020 to 2024
Virus Research,
Год журнала:
2025,
Номер
353, С. 199530 - 199530
Опубликована: Янв. 31, 2025
This
study
investigates
the
evolution
and
genetic
diversity
of
SARS-CoV-2
strains
circulating
in
Morocco
to
track
spread,
clade
distributions
mutations
virus
across
various
regions
from
February
2020
June
2024.
The
genome
sequences
were
retrieved
GISAID
database.
A
total
2630
analyzed
using
bioinformatic
tools
such
as
Nextclade,
followed
by
phylogenetic
statistical
analyses.
highlights
predominance
GRA
(Omicron
variant)
since
November
2021,
while
clades
G,
GH,
GR,
GRY
identified
earlier.
exhibited
highest
number
non-synonymous
mutations,
particularly
Spike
(S)
gene,
suggesting
strong
evolutionary
pressure.
correlation
analysis
between
structural
non-structural
proteins
revealed
key
interactions
S
NSP5,
providing
insights
into
viral
replication
assembly
processes.
work
gives
new
dynamics
underscores
importance
ongoing
genomic
surveillance
respond
emerging
variants
potential
future
outbreaks.
Язык: Английский
SARS-CoV-2 Alchemy: Understanding the dynamics of age, vaccination, and geography in the evolution of SARS-CoV-2 in India
PLoS neglected tropical diseases,
Год журнала:
2025,
Номер
19(3), С. e0012918 - e0012918
Опубликована: Март 10, 2025
Background
COVID-19
pandemic
had
unprecedented
global
impact
on
health
and
society,
highlighting
the
need
for
a
detailed
understanding
of
SARS-CoV-2
evolution
in
response
to
host
environmental
factors.
This
study
investigates
via
mutation
dynamics,
focusing
distinct
age
cohorts,
geographical
location,
vaccination
status
within
Indian
population,
one
nations
most
affected
by
COVID-19.
Methodology
Comprehensive
dataset,
across
diverse
time
points
during
Alpha,
Delta,
Omicron
variant
waves,
captured
essential
phases
pandemic’s
footprint
India.
By
leveraging
genomic
data
from
Global
Initiative
Sharing
Avian
Influenza
Data
(GISAID),
we
examined
substitution
landscape
three
demographic
segments:
children
(1–17
years),
working-age
adults
(18–64
elderly
individuals
(65+
years).
A
balanced
dataset
69,975
samples
was
used
study,
comprising
23,325
each
group.
design
ensured
high
statistical
power,
as
confirmed
power
analysis.
We
employed
bioinformatics
analyses,
explore
genetic
diversity
patterns
frequencies
groups.
Principal
findings
The
group
exhibited
notably
frequency
unique
substitutions,
suggesting
that
immune
pressures
highly
interactive
populations
may
accelerate
viral
adaptation.
Geographic
analysis
emphasizes
notable
regional
variation
rates,
potentially
driven
population
density
local
transmission
while
regions
with
more
homogeneous
strain
circulation
show
relatively
lower
rates.
also
revealed
significant
surge
substitutions
all
groups
period,
rates
remaining
elevated
even
after
widespread
vaccination,
compared
pre-vaccination
levels.
trend
supports
virus’s
adaptive
heightened
observed
through
increased
prevalence
important
genome
like
ORF1ab
Spike,
contributing
escape
transmissibility.
Conclusion
Our
affirm
importance
continuous
surveillance
evolution,
particularly
countries
research
provides
insights
anticipating
future
outbreaks
refining
preparedness
strategies,
thus
enhancing
our
capacity
proactive
responses.
Язык: Английский
Leveraging environmental microbial indicators in wastewater for data-driven disease diagnostics
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Ноя. 25, 2024
Wastewater-based
surveillance
(WBS)
is
an
emerging
tool
for
monitoring
the
spread
of
infectious
diseases,
such
as
SARS-CoV-2,
in
community
settings.
Environmental
factors,
including
water
quality
parameters
and
seasonal
variations,
may
influence
prevalence
viral
particles
wastewater.
This
study
aims
to
explore
relationships
between
these
factors
incidence
SARS-CoV-2
across
28
sites,
spanning
different
seasons
strata.
Язык: Английский
Recombination Events Among SARS-CoV-2 Omicron Subvariants: Impact on Spike Interaction With ACE2 Receptor and Neutralizing Antibodies
Evolutionary Bioinformatics,
Год журнала:
2024,
Номер
20
Опубликована: Янв. 1, 2024
The
recombination
plays
a
key
role
in
promoting
evolution
of
RNA
viruses
and
emergence
potentially
epidemic
variants.
Some
studies
investigated
the
occurrence
among
SARS-CoV-2,
without
exploring
its
impact
on
virus-host
interaction.
In
aim
to
investigate
burden
terms
frequency
distribution,
was
first
explored
44
230
Omicron
sequences
BQ
subvariants
under
investigation
“ML”
(Multiple
Lineages)
denoted
sequences,
using
3seq
software.
Second,
interaction
between
Spike
protein
ACE2
receptor
as
well
neutralizing
antibodies
(nAbs),
analyzed
docking
tools.
Recombination
detected
56.91%
82.20%
ML
strains,
respectively.
It
took
place
mainly
spike
ORF1a
genes.
For
recombinant
analysis
showed
that
interacted
strongly
with
weakly
nAbs.
mutations
S373P,
S375F
T376A
constitute
residue
network
enhances
RBD
ACE2.
Thirteen
(S373P,
S375F,
T376A,
D405N,
R408S,
K417N,
N440K,
S477N,
P494S,
Q498R,
N501Y,
Y505H)
NTD
(Y240H)
seem
be
implicated
immune
evasion
recombinants
by
altering
conclusion,
this
“in
silico”
study
demonstrated
mechanism
is
frequent
highlights
new
mutations,
enhancement
binding
(F376A)
escape
from
nAbs
(RBD:
F376A,
Y505H;
NTD:
Y240H).
Our
findings
present
considerable
insights
for
elaboration
effective
prophylaxis
therapeutic
strategies
against
future
SARS-CoV-2
waves.
Язык: Английский