Plant Viruses of Agricultural Importance: Current and Future Perspectives of Virus Disease Management Strategies

Phytopathology, Год журнала: 2022, Номер 113(2), С. 117 - 141

Опубликована: Сен. 12, 2022

Plant viruses cause significant losses in agricultural crops worldwide, affecting the yield and quality of products. The emergence novel or variants through genetic evolution spillover from reservoir host species, changes practices, mixed infections with disease synergism, impacts global warming pose continuous challenges for management epidemics resulting emerging plant virus diseases. This review describes some most devastating diseases plus select regional importance agriculturally important that have caused losses. lack curative measures prompts use risk-reducing managing These include exclusion, avoidance, eradication techniques, along vector practices. sensitive, high throughput, user-friendly diagnostic methods is crucial defining preventive strategies against viruses. advent next-generation sequencing technologies has great potential detecting unknown quarantine samples. deployment resistance crop plants an effective desirable method Several dominant recessive genes been used to manage crops. Recently, RNA-based such as dsRNA- siRNA-based RNA interference, microRNA, CRISPR/Cas9 provide transgenic nontransgenic approaches developing virus-resistant plants. Importantly, topical application dsRNA, hairpin RNA, artificial microRNA trans-active siRNA molecules on develop GMO-free methods. However, long-term efficacy acceptance these new technologies, especially methods, remain be established.

Язык: Английский

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Genome Editing for Sustainable Agriculture in Africa

Frontiers in Genome Editing, Год журнала: 2022, Номер 4

Опубликована: Май 12, 2022

Sustainable intensification of agriculture in Africa is essential for accomplishing food and nutritional security addressing the rising concerns climate change. There an urgent need to close yield gap staple crops enhance production feed growing population. In order meet increasing demand food, more efficient approaches produce are needed. All tools available toolbox, including modern biotechnology traditional, be applied crop improvement. The full potential new breeding such as genome editing needs exploited addition conventional technologies. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas)-based has rapidly become most prevalent genetic engineering approach developing improved varieties because its simplicity, efficiency, specificity, easy use. Genome improves variety by modifying endogenous free any foreign gene. Hence, genome-edited with no gene integration not regulated genetically modified organisms (GMOs) several countries. Researchers using CRISPR/Cas-based improving African biotic abiotic stress resistance quality. Many products, disease-resistant banana, maize resistant lethal necrosis, sorghum parasitic plant Striga enhanced quality, under development farmers. a creating enabling environment science-based regulatory guidelines release adoption products developed CRISPR/Cas9-mediated editing. Some progress been made this regard. Nigeria Kenya have recently published national biosafety regulation This article summarizes recent advances developments tools, applications crops, policies Africa.

Язык: Английский

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CRISPR/Cas systems for genomic Editing, biochemical Sensing, Bioanalysis, and diagnostics

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112638 - 112638

Опубликована: Янв. 1, 2025

Язык: Английский

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Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

Genes, Год журнала: 2021, Номер 12(12), С. 1881 - 1881

Опубликована: Ноя. 25, 2021

The breeding of tropical fruit trees for improving traits is complicated, due to the long juvenile phase, generation cycle, parthenocarpy, polyploidy, polyembryony, heterozygosity and biotic abiotic factors, as well a lack good genomic resources. Many molecular techniques have recently evolved assist hasten conventional efforts. Molecular markers linked development quality such shape, size, texture, aroma, peel pulp colour were identified in crops, facilitating Marker-assisted (MAB). An increase availability genome sequences fruits further aided discovery SNP variants/Indels, QTLs genes that can ascertain genetic determinants characters. Through multi-omics approaches genomics, transcriptomics, metabolomics proteomics, identification quantification transcripts, including non-coding RNAs, involved sugar metabolism, ripening, shelf life, stress impacts made possible. Utilizing assisted methods wide association (GWAS), selection (GS) modifications using CRISPR/Cas9 transgenics has paved way studying gene function developing cultivars with desirable by overcoming cycles. Such comprehensive related characters their applications strategies crop improvement are reviewed, discussed presented here.

Язык: Английский

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Control of Bacterial Diseases of Banana Using CRISPR/Cas-Based Gene Editing

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(7), С. 3619 - 3619

Опубликована: Март 25, 2022

Banana is an important staple food crop and a source of income for smallholder farmers in about 150 tropical sub-tropical countries. Several bacterial diseases, such as banana Xanthomonas wilt (BXW), blood, moko disease, cause substantial impacts on production. There vast yield gap the production bananas regions where pathogens several other pests are present together same field. BXW disease caused by campestris pv. musacearum reported to be most destructive East Africa. The affects all varieties grown region. Only wild-type diploid banana, Musa balbisiana, resistant disease. Developing disease-resistant one effective strategies manage diseases. Recent advances CRISPR/Cas-based gene editing techniques can accelerate improvement. Some progress has been made create resistance against using CRISPR/Cas9-mediated knocking out disease-causing susceptibility (S) genes or activating expression plant defense genes. A synopsis recent advancements perspectives application control diseases presented this article.

Язык: Английский

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Next‐generation methods for early disease detection in crops

Pest Management Science, Год журнала: 2023, Номер 80(2), С. 245 - 261

Опубликована: Авг. 21, 2023

Plant pathogens are commonly identified in the field by typical disease symptoms that they can cause. The efficient early detection and identification of essential procedures to adopt effective management practices reduce or prevent their spread order mitigate negative impacts disease. In this review, traditional innovative methods for plant highlighting major advantages limitations presented discussed. Traditional techniques diagnosis used pathogen focused typically on DNA, RNA (when molecular methods), proteins peptides serological methods) pathogens. Serological based mainly enzyme-linked immunosorbent assay (ELISA) most common method due high-throughput potential low cost. This technique is not particularly reliable sufficiently sensitive many during asymptomatic stage infection. For non-cultivable laboratory, nucleic acid-based technology best choice consistent identification. Lateral flow systems tools allow fast accurate results even conditions, but have sensitivity issues be overcome. PCR assays performed last-generation portable thermocyclers may provide rapid situ. advent instruments speed detection, commercial costs, potentially revolutionize pathology. review provides information current methodologies different © 2023 Authors. Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Язык: Английский

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CRISPR-Based Isothermal Next-Generation Diagnostic Method for Virus Detection in Sugarbeet

Frontiers in Microbiology, Год журнала: 2021, Номер 12

Опубликована: Июль 8, 2021

Rhizomania is a disease of sugarbeet caused by beet necrotic yellow vein virus (BNYVV) that significantly affects yield globally. Accurate and sensitive detection methods for BNYVV in plants field soil are necessary growers to make informed decisions on variety selection manage this disease. A recently developed CRISPR-Cas-based method has proven highly accurate human diagnostics. Here, we report the development CRISPR-Cas12a-based detecting roots sugarbeet. critical aspect technique identification conditions isothermal amplification viral fragments. Toward end, have reverse transcription (RT) recombinase polymerase (RPA) roots. The RT-RPA product was visualized, its sequence confirmed. Subsequently, designed validated cutting efficiency guide RNA targeting via vitro activity assay presence Cas12a. sensitivity CRISPR-Cas12a trans reporter-based determined using serially diluted synthetic target sequence. Further, root-tissue bait reared BNYVV-infested soil. results revealed specific infected relative healthy control as measured quantitatively through reporter signal. To our knowledge, first establishing RT-RPA- CRISPR-based diagnostic approaches from rhizomania diseased

Язык: Английский

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Genome editing for improving nutritional quality, post-harvest shelf life and stress tolerance of fruits, vegetables, and ornamentals

Frontiers in Genome Editing, Год журнала: 2023, Номер 5

Опубликована: Фев. 24, 2023

Agricultural production relies on horticultural crops, including vegetables, fruits, and ornamental plants, which sustain human life. With an alarming increase in population the consequential need for more food, it has become necessary increased to maintain food security. Conventional breeding subsidized development of improved verities but enhance crop production, new techniques be acquired. CRISPR-Cas9 system is a unique powerful genome manipulation tool that can change DNA precise way. Based bacterial adaptive immune system, this technique uses endonuclease creates double-stranded breaks (DSBs) at target loci under guidance single guide RNA. These DSBs repaired by cellular repair mechanism installs small insertion deletion (indels) cut sites. When equated alternate editing tools like ZFN, TALENs, meganucleases, CRISPR- The cas-based quickly gained fast-forward its simplicity, ease use, low off-target effect. In numerous industrial CRISPR technology been successfully used stress tolerance, self-life, nutritional improvements, flavor, metabolites. CRISPR-based most appropriate one with prospective goal generating non-transgenic yields avoiding regulatory hurdles release modified crops into market. Although several challenges horticultural, industrial, remain, novel nuclease, crop-specific application, makes dynamic improvement.

Язык: Английский

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Viral Contamination of Food

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 79 - 92

Опубликована: Янв. 1, 2025

Язык: Английский

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Biotechnological interventions in banana: current knowledge and future prospects

Heliyon, Год журнала: 2022, Номер 8(11), С. e11636 - e11636

Опубликована: Ноя. 1, 2022

Banana is an important food crop responsible for ensuring security, nutrition, and employment a significant portion of the world population. It has fairly broad genetic diversity distributed widely across globe. Due to its socio-economic importance, there been growing demand healthy improved planting materials banana. In recent years many companies organizations are working hard narrow down gap between supply quality materials. The other challenges includes susceptibility adverse environmental conditions, attack various pests/pathogens improvement nutritional bananas. To address these issues, refinement existing techniques introduction new experimental tools required. However, bananas large extent limited by using conventional methods due polyploidy, heterozygosity, sterility this plant. For rapid multiplication obtaining disease free plants, efficient in vitro propagation fine tuning protocols being tried laboratories Besides, developing successful protocol different cultivars bananas, deeper understanding factors associated with steps till transfer land immensely critical. Similarly, biotic abiotic stress tolerant banana enhancing commercial value through biotechnological interventions could be very useful. key intent review highlight research endeavor direction, future prospects.

Язык: Английский

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