The intracellular parasite Anncaliia algerae induces a massive miRNA down-regulation in human cells DOI Creative Commons
Reginald Florian Akossi, Frédéric Delbac, Hicham El Alaoui

и другие.

Non-coding RNA Research, Год журнала: 2023, Номер 8(3), С. 363 - 375

Опубликована: Май 18, 2023

Anncaliia algerae belongs to microsporidia, a group of obligate intracellular parasites related fungi. These are largely spread in water and food-webs can infect wide variety hosts ranging from invertebrates vertebrates including humans. In humans, microsporidian infections mainly opportunistic as immunocompetent clear naturally. Recent studies however have reported persistent highlighted them risk factor colon cancer. This may be direct result cell infection or an indirect effect the infectious microenvironment host's response. both cases, this raises question effects at host host-cell levels. We aimed address human response through transcriptomic kinetic study foreskin fibroblasts (HFF) infected with A.algerae, infecting microsporidia exceptionally range. focused solely on studying coding small non-coding miRNA expression. Our revealed generalized down-regulation miRNAs throughout up 547 different downregulated some timepoints also dysregulations that could facilitate parasite development immune lipid metabolism genes modulation. hypothesize possible nucleic acid expropriation explaining downregulation. work contributes better understanding dialogue occur between its cellular level, guide future biology unravel mode action these minimalist tissue levels.We generated comprehensive data set process help support comparative broader field parasitology. Lastly, results warrant for caution regarding exposure infections.

Язык: Английский

Transcriptomic Characterization of miRNAs in Apis cerana Larvae Responding to Ascosphaera apis Infection DOI Open Access
Yuxuan Song, Jian‐Feng Qiu, Jing Kang

и другие.

Genes, Год журнала: 2025, Номер 16(2), С. 156 - 156

Опубликована: Янв. 26, 2025

Ascosphaera apis is a fungal pathogen that specifically infects bee larvae, causing an outbreak of chalkbrood disease in the colony and decline number colonies. The role miRNA regulation honeybees response to A. infection unclear. In this study, based on small RNA-seq, we identified differentially expressed miRNAs (DEmiRNAs) their regulatory networks functions gut Apis cerana first day (AcT1), second (AcT2) third (AcT3) after infection, analyzed immune mechanism through miRNAs-mRNA network infection. A total 537 were obtained, 10, 27, 54 DEmiRNAs screened AcT1, AcT2, AcT3 groups, respectively. gradually increased with time. Stem-loop RT-PCR results showed most truly expressed, expression trend was consistent sRNA-seq. top five GO terms DEmiRNA-targeted mRNA binding, cellular process, catalytic activity, metabolic single-organism process. main pathways enriched by KEGG endocytosis, ubiquitin-mediated proteolysis, phagosome, JAK-STAT immune-related signaling pathways. target mRNAs these related pathway genes miRNA-mRNA analysis ace-miR-539-y core early larvae infected ace-miR-1277-x late phagosome. participated regulating host’s energy metabolism, immunity, humoral immunity. study provide basis for c. new insights into host-pathogen interactions.

Язык: Английский

Процитировано

0
Distinct virulence of the microsporidian parasite in honey bees competing habitat DOI Creative Commons
Xiuxiu Wei, Qiang Huang

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Фев. 17, 2025

In natural ecosystems, parasites often infect multiple host species, particularly when hosts share habitats, facilitating host-to-host transmission and altering traditional host-parasite coevolution dynamics. This study examines the microsporidian parasite Nosema ceranae in Eastern honey bees ( Apis cerana ) Western mellifera ), assessing its virulence proliferation Using inoculation experiments, we measured bee mortality spore loads to infer proliferation. Additionally, time-series transcriptome analysis of both provide insights into host-pathogen interactions. The results reveal that N. produces more spores with lower A. but causes higher production . also suppresses gene expression, stronger suppression observed These findings suggest is adapted for low high exhibits limited highlights evolution distinct trade-offs between a multi-host system, offering valuable parasite-host dynamics their ecological implications.

Язык: Английский

Процитировано

0
Analysis of the Expression Patterns of piRNAs in Response to Microsporidian Invasion in Midgut of Workers (Apis cerana cerana) DOI Open Access
Yiqiong Zhang, Mengyi Wang, Wenhua Xu

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2402 - 2402

Опубликована: Март 7, 2025

Piwi-interacting RNAs (piRNAs) play an essential part in transposon suppression, DNA methylation, and antiviral responses. The current understanding of the roles piRNAs honeybees is very limited. This study aims to analyze expression pattern regulatory role Asian honeybee (Apis cerana) responding infection by Nosema ceranae, based on previously gained small RNA-seq data. Here, 450 422 were respectively identified midgut tissues Apis cerana workers at 7 10 days post-inoculation (dpi) with N. including 539 non-redundant ones. Additionally, one up-regulated (piR-ace-1216942) down-regulated (piR-ace-776728) piRNA detected workers’ dpi, targeting 381 mRNAs involved 31 GO terms, such as metabolic processes, catalytic activity, organelles, well 178 KEGG pathways, lysosome, MAPK signaling pathway, purine metabolism. A total 35 11 screened from 13,511 engaged 50 biological regulation, transporter membrane, 389 JAK-STAT Hippo nitrogen Further analysis indicated that 28 differentially expressed (DEpiRNAs) dpi could target 299 annotated three cellular immune pathways (lysosome, endocytosis, phagosome), while 24 DEpiRNAs 205 relevant four humoral (FoxO, JAK-STAT, NF-κB, pathway). Through Sanger sequencing RT-qPCR, six randomly selected was verified. Moreover, dual-luciferase reporter gene assay confirmed binding relationships between piR-ace-446232 CRT piR-ace-1008436 EGFR. Our findings not only contribute enrich our but also provide a basis for exploring host response ceranae mediated piRNAs.

Язык: Английский

Процитировано

0
Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata DOI Creative Commons

Sarah Dametto,

Benjamin Gourbal, Cristian Chaparro

и другие.

Current Research in Parasitology and Vector-Borne Diseases, Год журнала: 2025, Номер unknown, С. 100262 - 100262

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts DOI Creative Commons
Xiaoxue Fan, Wende Zhang,

Sijia Guo

и другие.

Insects, Год журнала: 2023, Номер 14(5), С. 469 - 469

Опубликована: Май 16, 2023

MiRNAs, as a kind of key regulators in gene expression, play vital roles numerous life activities from cellular proliferation and differentiation to development immunity. However, little is known about the regulatory manner miRNAs Asian honey bee (Apis cerana) guts. Here, on basis our previously gained high-quality transcriptome data, transcriptome-wide identification larval guts Apis cerana was conducted, followed by investigation miRNAs' differential expression profile during gut development. In addition network, potential function differentially expressed (DEmiRNAs) further analyzed. total, 330, 351, 321 were identified 4-, 5-, 6-day-old guts, respectively; among these, 257 shared, while 38, 51, 36 ones specifically expressed. Sequences six confirmed stem-loop RT-PCR Sanger sequencing. Additionally, "Ac4 vs. Ac5" comparison group, there seven up-regulated eight down-regulated miRNAs; these DEmiRNAs could target 5041 mRNAs, involving series GO terms KEGG pathways associated with growth development, such process, cell part, Wnt, Hippo. Comparatively, four detected "Ac5 Ac6" group targets diverse development-related pathways, including cell, organelle, Notch Wnt. Intriguingly, it noticed that miR-6001-y presented continuous up-regulation trend across developmental process implying may be essential modulator Further indicated 43 31 engaged several crucial development-associated signaling Hippo, Notch. Ultimately, trends five randomly selected verified using RT-qPCR. These results demonstrated dynamic structural alteration accompanied A. c. likely participate modulation well affecting critical via regulation genes. Our data offer for elucidating mechanism underlying

Язык: Английский

Процитировано

6
The intracellular parasite Anncaliia algerae induces a massive miRNA down-regulation in human cells DOI Creative Commons
Reginald Florian Akossi, Frédéric Delbac, Hicham El Alaoui

и другие.

Non-coding RNA Research, Год журнала: 2023, Номер 8(3), С. 363 - 375

Опубликована: Май 18, 2023

Anncaliia algerae belongs to microsporidia, a group of obligate intracellular parasites related fungi. These are largely spread in water and food-webs can infect wide variety hosts ranging from invertebrates vertebrates including humans. In humans, microsporidian infections mainly opportunistic as immunocompetent clear naturally. Recent studies however have reported persistent highlighted them risk factor colon cancer. This may be direct result cell infection or an indirect effect the infectious microenvironment host's response. both cases, this raises question effects at host host-cell levels. We aimed address human response through transcriptomic kinetic study foreskin fibroblasts (HFF) infected with A.algerae, infecting microsporidia exceptionally range. focused solely on studying coding small non-coding miRNA expression. Our revealed generalized down-regulation miRNAs throughout up 547 different downregulated some timepoints also dysregulations that could facilitate parasite development immune lipid metabolism genes modulation. hypothesize possible nucleic acid expropriation explaining downregulation. work contributes better understanding dialogue occur between its cellular level, guide future biology unravel mode action these minimalist tissue levels.We generated comprehensive data set process help support comparative broader field parasitology. Lastly, results warrant for caution regarding exposure infections.

Язык: Английский

Процитировано

1