Cell Reports, Год журнала: 2024, Номер 43(11), С. 114910 - 114910
Опубликована: Окт. 30, 2024
Язык: Английский
Процитировано
5
Plants, Год журнала: 2025, Номер 14(3), С. 435 - 435
Опубликована: Фев. 2, 2025
Wheat (Triticum aestivum L.) is a primary crop globally. Among the numerous pathogens affecting wheat production, Puccinia striiformis f. sp. tritici (Pst) significant biotic stress agent and poses major threat to world food security by causing stripe rust or yellow disease. Understanding molecular basis of plant–pathogen interactions crucial for developing new means disease management. It well established that effector proteins play pivotal role in pathogenesis. Therefore, studying has become an important area research plant biology. Our previous work identified differentially expressed candidate secretory based on transcriptome sequencing data from susceptible (Avocet S) resistant YR10) infected with Pst. secreted proteins, PSTG_14090 contained ancient double-psi beta-barrel (DPBB) fold, which conserved rare lipoprotein A (RlpA) superfamily. This study investigated immune responses, encodes protein, here referred as Pst-DPBB, having 131 amino acids predicted signal peptide (SP) 19 at N-terminal end, DNA sequence this highly among different races. qRT-PCR analysis indicated expression levels are upregulated during early stages infection. Subcellular localization studies Nicotiana benthamiana leaves protoplasts revealed it distributed cytoplasm, nucleus, apoplast. We demonstrated Pst-DPBB negatively regulates response functioning various compartments cells. Based Co-IP structural predictions putative interaction analyses AlphaFold 3, we propose probable biological function(s). behaves papain inhibitor cysteine protease; high homology kiwellin, known interact chorismate mutase, suggesting inhibits native function host mutase involved salicylic acid synthesis. The DPBB fold also RNA, may suggest its possible regulating gene expression.
Язык: Английский
Процитировано
0
Journal of soil science and plant nutrition, Год журнала: 2025, Номер unknown
Опубликована: Апрель 29, 2025
Язык: Английский
Процитировано
0
Frontiers in Plant Science, Год журнала: 2025, Номер 16
Опубликована: Май 21, 2025
Plant disease poses a great threat to crop production. The mechanisms underlying plant-pathogen interactions are critical research topics worldwide. In recent years, significant breakthrough studies have been reported, broadening our understanding of plant immunity. Based on these findings, many strategies developed improve defense against various diseases. Here, we summarize and their applications in aimed at promoting resistance. Besides domain swapping, gene shuffling, random mutation, three additional the last decade. first strategy is editing host susceptibility (S) genes prevent pathogen infection. Editing Mlo DMR6 species good examples this approach. second promoters S or resistance (R) genes. This widely used counteract Xanthomonas, such as modifying LOB1 SWEET several crops enhance third designing R products, especially nucleotide-binding leucine-rich repeat (NLR) receptors. approach based growing knowledge structural features NLRs, which seen advances recently. To date, all NLR-engineering attempts focused rice paired Pikp-1/Pikp-2 (allelic Pikm-1/Pikm-2) RGA4/RGA5. bioengineering NLRs provides promising method combat diverse pathogens. Detailed also discussed review, organized around strategies. summary, with progresses immune mechanism, innovative molecular available mitigate pathogens future.
Язык: Английский
Процитировано
0
Plants, Год журнала: 2024, Номер 13(5), С. 668 - 668
Опубликована: Фев. 28, 2024
The recognition of pathogen effectors through the nucleotide-binding leucine-rich repeat receptor (NLR) family is an important component plant immunity. In addition to typical domains such as TIR, CC, NBS, and LRR, NLR proteins also contain some atypical integrated (IDs), roles which are rarely investigated. Here, we carefully screened soybean (Glycine max) genome identified IDs that appeared in TNL-like proteins. Our results show multiple (36) widely present A total 27 Gm-TNL-ID genes (soybean gene encoding ID) were cloned their antiviral activity towards mosaic virus (SMV)/tobacco (TMV) was verified. Two resistance (R) genes, SRA2 (SMV contains AAA_22 domain) SRZ4 zf-RVT domain), possess broad-spectrum characteristics six viruses including SMV, TMV, plum pox (PPV), cabbage leaf curl (CaLCuV), barley stripe (BSMV), tobacco rattle (TRV). effects Gm-TNL-IDX (the domain after TN on a R protein SRC7TN (we previously reported SRC7) validated, most inhibits mediated by SRC7TN, possibly intramolecular interactions. Yeast-two-hybrid (Y2H) bimolecular fluorescence complementation (BiFC) assays showed seven interacted with SMV-component Truncation analysis indicated SRZ4TIR sufficient mediate against SMV. Soybean cDNA library screening 48 interacting summary, our indicate integration widespread frequent. NLR-ID toolkit provide expected be valuable for elucidating functions immune system lay foundation development engineering plant-disease control future.
Язык: Английский
Процитировано
3
Frontiers in Plant Science, Год журнала: 2023, Номер 14
Опубликована: Апрель 25, 2023
Citrus chlorotic dwarf-associated virus (CCDaV) is a Citlodavirus species in the Geminiviridae family that causes tremendous economic loss to citrus industry China. Some proteins encoded by geminiviruses are crucial for interaction between and its host plant. However, exact functions of CCDaV-encoded such as CCDaV-RepA have not been investigated. This study presents evidence elicits hypersensitive response (HR)-like cell death Nicotiana benthamiana was accompanied production H2O2 ion leakage, which suggested potential recognition target inducing defense responses. Furthermore, rolling-circle replication motifs associated with triggering HR-like N. benthamiana. Confocal microscopy deletion mutagenesis assays showed located nucleus, while first eight amino acids (aa) at N terminus two regions aa residues 122-263 220-264 RepA were nuclear localization. Tobacco rattle virus-induced gene silencing key signaling cascade components revealed induced inhibited WRKY1-silenced Moreover, WRKY1 expression upregulated RepA-GFP infiltrated Overall, results suggest NbWRKY1 positively regulated -induced These findings provide novel information further research on interactions CCDaV
Язык: Английский
Процитировано
6
BMC Plant Biology, Год журнала: 2024, Номер 24(1)
Опубликована: Май 8, 2024
Abstract Background Potato virus Y (PVY) is among the economically most damaging viral pathogen in production of potato ( Solanum tuberosum ) worldwide. The gene Ry sto derived from wild relative stoloniferum confers extreme resistance to PVY. Results presence and diversity were investigated relatives (298 genotypes representing 29 accessions 26 tuber-bearing species) using PacBio amplicon sequencing. A total 55 unique Rysto-like sequences identified 72 12 10 species six resistant controls (potato cultivars Alicja, Bzura, Hinga, Nimfy, White Lady breeding line PW363). showed 89.87 99.98% nucleotide identity reference gene, these encoded 45 protein sequences. While Rysto-like26 Rysto-like16 PW363 encode a identical reference, remaining 44 predicted proteins 65.93 99.92% reference. Higher levels found than control cultivars. TIR NB-ARC domains conserved within proteins, while LRR C-JID more variable. Several species, including S. antipoviczii hougasii , This study demonstrated Hyoscyamus niger Solanaceae distantly related as host Conclusions new variants PVY are potential sources against breeding. Identification H. for important cultivation this plant, studies on management, its ecology, migrations. sequencing based SMRT following data analysis pipeline described our work may be applied obtain analyze any full-length genes any, even polyploid, organisms.
Язык: Английский
Процитировано
1
BMC Research Notes, Год журнала: 2024, Номер 17(1)
Опубликована: Окт. 6, 2024
Abstract Objective This research aims to analyze the presence and distribution of resistance genes in avium fruticosa subgenomes Prunus cerasus through computational methods bioinformatics tools. Results Analysis genome transcriptome sequencing data revealed a total 19,570 transcripts with at least one gene domain subgenome 19,142 . Key findings include identification 804 “complete” 817 , distinct distributions classes observed between subgenomes. Phylogenetic analysis showed clustering genes, unique proteins were identified each subgenome. Functional annotation comparisons Arabidopsis thaliana highlighted shared emphasizing complexity disease cherry species. Additionally, higher diversity RLKs RLPs was observed, 504 18 showing similarity known reference genes.
Язык: Английский
Процитировано
1
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Март 1, 2024
Язык: Английский
Процитировано
0
Vestnik Moskovskogo universiteta Seria 16 Biologia, Год журнала: 2024, Номер 79(№4, 2024), С. 287 - 297
Опубликована: Янв. 1, 2024
Studies were performed on the Arabidopsis double mutant plants parg1 exa1 characterized by cell death along leaf margin, activation of expression immune receptor genes, PR-genes and hypersensitive response which is a sign autoimmunity. At same time, unlike other autoimmunity mutations, demonstrated acquisition pluripotency cells ability to regenerate new margins. Because unusual phenotype, this study analyzed in depth transcriptome young leaves wild-type plants. The was found have increased levels all major components response, including genes for Ca2+ conducting channels, NADPH oxidases, salicylic acid synthesis jasmonic acid, many others. findings suggest that PARG1 EXA1 control level poly(ADP-ribosylation) process nonsensemediated RNA degradation, respectively, are important regulators response. Their function essential prevent overactivation defense systems
Язык: Английский
Процитировано
0