Genetic Landscape and Mitochondrial Metabolic Dysregulation in Patients Suffering From Severe Long COVID

Journal of Medical Virology, Год журнала: 2025, Номер 97(3)

Опубликована: Март 1, 2025

ABSTRACT Long COVID represents a significant global health challenge with an unclear etiology. Alongside accumulating evidence of mitochondrial dysfunction in patients acute SARS‐CoV‐2 infection, symptomatic overlap exists between long and disorders. However, the genetic underpinnings have not been previously explored. We employed whole genome sequencing to analyze 13 severe identify defects related function. performed extracellular bioenergetics flux analysis on peripheral blood mononuclear cells proteomics evaluate cellular compared results those healthy controls. Our investigation identified 10 variants classified as pathogenic or likely 83 unknown significance affecting wide range mitochondria‐associated biological functions. Bioenergetics revealed altered ATP production rate four This study presents initial potential underlying predisposition while demonstrating energy capacity subset these patients. These findings open avenues for further research into role pathology suffering from may pave way targeted therapeutic strategies aimed at mitigating dysfunction.

Язык: Английский

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Viral inactivation of murine coronavirus via multiple gas plasma-derived reactive species

Redox Biology, Год журнала: 2025, Номер 82, С. 103591 - 103591

Опубликована: Март 11, 2025

The recent pandemic has highlighted the urgent need to elucidate pathophysiological mechanisms underlying viral effects in humans and is driving search for innovative antiviral therapies. Several studies have investigated ability of gas plasma, a partially ionized that simultaneously generates several reactive species, be new tool. However, aspects action plasma remained elusive. In this study, we, first time, used device approved medical purposes routinely applied clinics, especially wound healing, test its activity against murine corona-virus vitro (MHV-GFP), research model analogous human coronaviruses such as SARS-CoV-2. For this, we established novel high-content imaging assay gave quantitative kinetic information about infection reduced fibroblasts (17Cl-1) host cells. Gas treatment delayed infectivity overall toxicity 17Cl1 Various antioxidants at different concentrations were screened identify ROS relevant effects. Catalase provided no virus protection, DMSO, mannitol, histidine, Trolox, ascorbic acid only modestly virucidal efficacy. By contrast, glutathione, tyrosine, cysteine showed profound but not complete protection MHV from plasma-derived suggesting pivotal roles superoxide radicals peroxynitrite plasma-driven inactivation. At extended exposure times, fewer intact RNA detected, indicative species-driven modifications or degradation an additional mechanism action. Virus particle size changes measured by electron microscopy moderate. Collectively, identified potent clinically argon jet along with potential

Язык: Английский

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Plant-derived Bisphenol C is a drug candidate against Nipah henipavirus infection: an in-vitro and in-silico study of Pouzolzia zeylanica (L.) Benn.

In Silico Pharmacology, Год журнала: 2025, Номер 13(1)

Опубликована: Март 14, 2025

Язык: Английский

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Lithium Chloride Modulates Inflammatory and Apoptotic Responses in Lung Adenocarcinoma Cells Challenged with SARS-CoV-2 S1-His Protein Recombinant

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Background: In 2019, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) began COVID-19 pandemic, resulting in millions of deaths worldwide. Lung cancer patients infected with SARS-CoV-2 were found to be particularly vulnerable and fatal outcomes. This study aimed explore whether lithium chloride (LiCl), a drug commonly used treat mental disorders, could serve as potential inhibitor inflammatory markers lung adenocarcinoma cells exposed glycoprotein. Methods: The in vitro cytotoxic effects assessed using Muse^® Ki67 proliferation assay, cell death, cycle analysis. Nitric oxide measurement oxidative stress IL-6, NF-κB human inflammation antibody array membrane performed evaluate parameters after LiCl treatments. Results: spike protein stimulated ROS NO production, treatment low concentrations reduced percentage stress. Cell death assay showed that late apoptotic protein. analysis increased S phase population A549 G2/M populations. individually IL-6 expression however, co-treatment levels. had strong positive spots for 26 targets their intensity. Conclusion: These findings suggest is promising candidate targeting pathway triggered by Additionally, promotes early apoptosis induces arrest presents therapeutic approach utilizing management infection patients.

Язык: Английский

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Potential Roles of Natural Antioxidants in Modulating Neurodegenerative Disease Pathways

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Язык: Английский

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