Genomic characterization of Listeria monocytogenes isolated from normally sterile human body fluids in Lithuania from 2016 to 2021

Microbial Genomics, Год журнала: 2025, Номер 11(5)

Опубликована: Май 20, 2025

Listeria monocytogenes is a saprophytic gram-positive bacterium and opportunistic foodborne pathogen that can cause listeriosis in humans. The incidence of has been rising globally and, despite antimicrobial treatment, the mortality rates associated with most severe forms such as sepsis, meningitis meningoencephalitis remain high. notification humans mandatory Lithuania, up to 20 cases are reported annually. However, no studies have described detailed virulence susceptibility profiles any clinical L. strains Lithuania. Accordingly, this study aimed describe antibiotic invasive perform in-depth characterization isolated from patients neuroinfections through whole-genome sequencing. A total 70 isolates were collected, mostly infected aged 65 or older, between 2016 2021 : 41 (58.6%) blood, 19 (27.1%) cerebrospinal fluid, 5 (7.1%) wounds, 1 (1.4%) pleural fluid brain abscess. Two phylogenetic lineages identified—I ( n = 16/70, 22.9%) II 54/70, 77.1%)—along three serogroups—IIa 53/70, 75.7%), IVb 22.9%), IIc 1/70, 1.4%). Genomic analysis showed high level diversity seven genotypes: ST6 6), ST155 5), ST8 4), ST504 2) singletons for ST37, ST451 ST2. Phylogenetic clustered these into two clades defined by serogroups IIa. Notably, five tightly together (difference 6–48 core SNPs reference 0, 4 44 each other) ST155, previously European outbreak. Comparison publicly available genomes did not identify unique clusters genotypes. No acquired resistance genes identified. Our highlights complementary value sequencing routine PCR-based surveillance This first characterize compare Lithuania using retrospective detection clone underscores need review strengthening epidemiological strategies non-clinical settings

Язык: Английский

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Effects of Chitosan Coatings on Controlling Listeria monocytogenes and Methicillin-Resistant Staphylococcus aureus in Beef and Mutton Cuts

Applied Sciences, Год журнала: 2022, Номер 12(22), С. 11345 - 11345

Опубликована: Ноя. 8, 2022

Ruminant meat is considered to be a potent vehicle of foodborne pathogen transmission. Edible coatings are promising for enhancing safety. Here, edible chitosan membranes were applied whole cuts beef and mutton test the survival pathogenic bacteria Listeria monocytogenes methicillin-resistant Staphylococcus aureus. Meat pieces weighing approximately 200 g inoculated with cocktail three S. aureus isolates or four L. (6.00 log10CFU/g). The encased in coating formed by immersion incubated aerobically vacuum-packaged LDPE/PA/LDPE bags up 21 days. A decrease both (x = −1.95 log10CFU/g, standard error 0.23 log10CFU/g) counts −1.07 0.26 was observed. No significant differences observed between monocytogenes-spiked pieces; statistically higher versus under similar treatments. Aerobic storage enhanced antibacterial effects chitosan—a trend that not According results, effective controlling growth monocytogenes.

Язык: Английский

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Cross-contamination of mature Listeria monocytogenes biofilms from stainless steel surfaces to chicken broth before and after the application of chlorinated alkaline and enzymatic detergents

Food Microbiology, Год журнала: 2023, Номер 112, С. 104236 - 104236

Опубликована: Фев. 8, 2023

The objectives of this study were, firstly, to compare a conventional (i.e., chlorinated alkaline) versus an alternative (chlorinated alkaline plus enzymatic) treatment effectivity for the elimination biofilms from different L. monocytogenes strains (CECT 5672, CECT 935, S2-bac and EDG-e). Secondly, evaluate cross-contamination chicken broth non-treated treated formed on stainless steel surfaces. Results showed that all were able adhere develop at approximately same growth levels (≈5.82 log CFU/cm2). When put into contact with model food, obtained average transference rate potential global 20.4%. Biofilms detergent rates similar as high number residual cells around 4 5 Log CFU/cm2) present surface, except EDG-e strain which diminished 0.45%, was related protective matrix. Contrarily, shown not produce due its biofilm control (<0.50% transference) 935 had behavior. Therefore, changing more intense cleaning treatments in processing environments can reduce risk cross-contamination.

Язык: Английский

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Comparative genomics analysis to explore the biodiversity and mining novel target genes of Listeria monocytogenes strains from different regions

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Июнь 19, 2024

As a common foodborne pathogen, infection with L. monocytogenes poses significant threat to human life and health. The objective of this study was employ comparative genomics unveil the biodiversity evolutionary characteristics strains from different regions, screening for potential target genes mining novel genes, thus providing reference value specific molecular detection therapeutic targets strains. Pan-genomic analysis revealed that regions have open genomes, solid genetic basis adaptation environments. These contain numerous virulence contribute their high pathogenicity. They also exhibit relatively resistance phosphonic acid, glycopeptide, lincosamide, peptide antibiotics. results mobile elements indicate that, despite being located in geographical locations, there is certain degree similarity bacterial genome evolution environmental pressures. identified through pan-genomics are primarily associated fundamental activities invasion monocytogenes, including known such as inlB, which can be utilized purposes. After large number we further screened them using hub gene selection methods genes. present employed eight methods, ultimately identifying ten highly connected (bglF_1, davD, menE_1, tilS, dapX, iolC, gshAB, cysG, trpA, hisC), play crucial roles pathogenesis monocytogenes. pan-genomic showed evolution. PCR demonstrated excellent specificity bglF_1 davD Therefore, hold promise regions.

Язык: Английский

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A novel variant of the Listeria monocytogenes type VII secretion system EssC component is associated with an Rhs toxin

Microbial Genomics, Год журнала: 2023, Номер 9(6)

Опубликована: Июнь 6, 2023

The type VIIb protein secretion system (T7SSb) is found in Bacillota (firmicute) bacteria and has been shown to mediate interbacterial competition. EssC a membrane-bound ATPase that critical component of the T7SSb plays key role substrate recognition. Prior analysis available genome sequences foodborne bacterial pathogen Listeria monocytogenes although was encoded as part core genome, could be one seven different sequence variants. While each variant associated with specific suite candidate proteins immediately downstream essC , many LXG-domain were across multiple Here, we have extended this using diverse collection 37 930 L . genomes. We identified rare eighth present ten L. lineage III These genomes also encode large toxin rearrangement hotspot (Rhs) repeat family adjacent essC8 along probable immunity three small accessory proteins. further nine novel proteins, four additional chromosomal hotspots where LXG can encoded. eight variants other species, types identified. Across genus, species frequently types, indicating diversity primary feature genus

Язык: Английский

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A novel multiplex PCR based method for the detection of Listeria monocytogenes clonal complex 8

International Journal of Food Microbiology, Год журнала: 2023, Номер 409, С. 110475 - 110475

Опубликована: Ноя. 6, 2023

Язык: Английский

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Characterisation of the growth behaviour of Listeria monocytogenes in Listeria synthetic media

Environmental Microbiology Reports, Год журнала: 2023, Номер 15(6), С. 669 - 683

Опубликована: Окт. 20, 2023

The foodborne pathogen Listeria monocytogenes can grow in a wide range of environmental conditions. For the study physiology this organism, several chemically defined media have been developed over past decades. Here, we examined ability L. wildtype strains EGD-e and 10403S to under salt pH stress synthetic medium (LSM). Furthermore, determined that carbon sources could support growth both LSM. However, for hexose phosphate sugars such as glucose-1-phosphate, need be pre-grown conditions, where major virulence regulator PrfA is active. In addition, was observed when LSM supplemented with amino acid sugar N-acetylmannosamine (ManNAc). We were able show some proteins encoded operon lmo2795-nanE, ManNAc-6-phosphate epimerase NanE, are required presence ManNAc. first gene operon, lmo2795, encodes transcriptional RpiR family. Using electrophoretic mobility shift assays quantitative real-time PCR analysis, Lmo2795 binds promoter region lmo2795-nanE activates its expression.

Язык: Английский

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Relevant foodborne bacteria associated to pork production chain

Advances in food and nutrition research, Год журнала: 2024, Номер unknown, С. 181 - 218

Опубликована: Окт. 8, 2024

Язык: Английский

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Characterization of the growth behavior ofListeria monocytogenesinListeriasynthetic media

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Фев. 22, 2023

Abstract The foodborne pathogen Listeria monocytogenes can grow in a wide range of environmental conditions. For the study physiology this organism, several chemically defined media have been developed over past decades. Here, we examined ability L. wildtype strains EGD-e and 10403S to under salt pH stress synthetic medium (LSM). Furthermore, determined that carbon sources could support growth both LSM. However, for hexose phosphate sugars such as glucose-1-phosphate, need be pre-grown conditions, where major virulence regulator PrfA is active. In addition, was observed when LSM supplemented with amino acid sugar N -acetylmannosamine (Man Ac). We were able show some proteins encoded operon lmo2795 - nanE , Man Ac-6-phosphate epimerase NanE, are required presence Ac. first gene operon, lmo2795, encodes transcriptional RpiR family. Using electrophoretic mobility shift assays quantitative real time PCR analysis, Lmo2795 binds promoter region activates its expression. Originality-Significance Statement Current knowledge survival human diverse conditions mostly generated complex medium, condition rarely found environment or host pathogen. Our work contributes characterization grown nutrient limiting requirements regards metabolizable sources.

Язык: Английский

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De rol van transmissieroutes water en mest bij besmetting van groente en fruit productiesystemen met humaan pathogene bacteriën

Опубликована: Янв. 1, 2024

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