Assessment of in vitro activity of ceftazidime/avibactam on carbapenemase‐producing Enterobacterales from Iran: An experimental study DOI Creative Commons
Mehri Haeili,

Parisa Ghaderi Bavil‐Olyaei

Health Science Reports, Год журнала: 2024, Номер 7(8)

Опубликована: Авг. 1, 2024

Abstract Background and Aims The prevalence of carbapenemase‐producing Enterobacterales (CPE) continues to increase worldwide. Combination β‐lactam novel β‐lactamase inhibitors introduce a revolutionary treatment option for CPE. Ceftazidime/avibactam (CAZ/AVB) has been recently developed severe infections caused by multidrug‐resistant bacteria. We aimed evaluate in vitro activity CAZ/AVB on collection 85 ESBL‐producing‐carbapenemase negative CPE from Iran. Methods ESBL carbapenemase production was phenotypically confirmed combined disk test modified carbapenem inactivation method respectively. presence clinically important encoding genes examined using PCR. Susceptibility all isolates determined discs containing 30 μg ceftazidime +20 avibactam (AVB). Minimum inhibitory concentrations (MICs) 28 (4 Escherichia coli 24 Klebsiella pneumoniae ) gradient diffusion MIC strips (0.016−256 mg/L +4 AVB). Results All identified positive‐carbapenemase were found be susceptible CAZ/AVB. Among the resistant isolates, showed potent against OXA‐48‐like (MIC ranges 0.125/4−0.75/4 mg/L) KPC positive <0.016/4−0.19/4 mg/L). However, AVB could not restore ceftazdime producing metallo‐β‐lactamases (MLBs) including VIM, NDM > 256/4 IMP 8/4 Conclusion Our data highlighted excellent performance ESBL‐producing suggesting that this combination can efficiently used as therapeutic management particularly regions with high and/or but MBL‐negative .

Язык: Английский

Insights into the Microbiome and Antibiotic Resistance Genes from Hospital Environmental Surfaces: A Prime Source of Antimicrobial Resistance DOI Creative Commons
Alfizah Hanafiah, Asif Sukri,

Hamidah Yusoff

и другие.

Antibiotics, Год журнала: 2024, Номер 13(2), С. 127 - 127

Опубликована: Янв. 26, 2024

Hospital environmental surfaces are potential reservoirs for transmitting hospital-associated pathogens. This study aimed to profile microbiomes and antibiotic resistance genes (ARGs) from hospital using 16S rRNA amplicon metagenomic sequencing at a tertiary teaching in Malaysia. Samples were collected patient sinks healthcare staff counters surgery orthopaedic wards. The samples’ DNA subjected shotgun identify bacterial taxonomic profiles, genes, virulence factor pathways. richness was more diverse the samples than those counters. Proteobacteria Verrucomicrobia dominated phylum level, while Bacillus, Staphylococcus, Pseudomonas, Acinetobacter genus level. Staphylococcus epidermidis aureus prevalent on Bacillus cereus counter samples. highest counts of ARGs beta-lactam detected, followed by against fosfomycin cephalosporin. We report detection mcr-10.1 that confers colistin setting gene pathways aid transfer between bacteria identified. Environmental serve as nosocomial infections require mitigation strategies control spread bacteria.

Язык: Английский

Процитировано

4
A Mini-Review of Anti-Listerial Compounds from Marine Actinobacteria (1990–2023) DOI Creative Commons

Siyanda S. Ngema,

Evelyn Madoroba

Antibiotics, Год журнала: 2024, Номер 13(4), С. 362 - 362

Опубликована: Апрель 15, 2024

Among the foodborne illnesses, listeriosis has third highest case mortality rate (20–30% or higher). Emerging drug-resistant strains of Listeria monocytogenes, a causative bacterium listeriosis, exacerbate seriousness this public health concern. Novel anti-Listerial compounds are therefore needed to combat challenge. In recent years, marine actinobacteria have come be regarded as promising source novel antimicrobials. Hence, our aim was provide narrative available literature and discuss trends regarding bioprospecting for new compounds. Four databases were searched review: Academic Search Ultimate, Google Scholar, ScienceDirect, South African Thesis Dissertations. The search restricted peer-reviewed full-text manuscripts that discussed antimicrobials written in English from 1990 December 2023. total, past three decades (1990–December 2023), only 23 been tested their potential. Out reported compounds, 2-allyoxyphenol, adipostatins E–G, 4-bromophenol, ansamycins (seco-geldanamycin B, 4.5-dihydro-17-O-demethylgeldanamycin, seco-geldanamycin) found possess activity. Thus, survey reveals scarcity published assays testing capacity bioactive sourced during period.

Язык: Английский

Процитировано

3
Antibiotic Residues in Milk and Milk-Based Products Served in Kuwait Hospitals: Multi-Hazard Risk Assessment DOI Creative Commons

Mariam N Alenezi,

Yasmine H. Tartor,

Mohammed Elsherbini

и другие.

Antibiotics, Год журнала: 2024, Номер 13(11), С. 1073 - 1073

Опубликована: Ноя. 11, 2024

Antimicrobial resistance (AMR) poses a significant global health challenge affecting food safety and development. Residues of antibiotics in from animal sources, particularly milk, contribute to the development spread AMR, alter intestinal microbiota, potentially lead allergies, serious conditions, environmental technological problems within dairy industry. Therefore, this study investigated residue levels veterinary drugs β-lactam tetracyclines milk products assessed human risks. Two hundred product samples (pasteurized sterile soft white cheese, processed 50 each) were collected different hospitals State Kuwait screened for antibiotic residues using microbial inhibition assay (Delvotest SP-NT) high-performance liquid chromatography (HPLC). Delvotest SP-NT HPLC analyses showed that 30, 28, 26, 24% pasteurized sterilized cheese tested positive residues. Forty-eight confirmed as by both methods, six initially found be negative HPLC. Multi-antibiotic detected five The kappa coefficient (0.921;

Язык: Английский

Процитировано

3
Structure and mechanism basis of β-lactam activity against Mycobacterium tuberculosis: A review of literature DOI
Nazia Ahmad, Zeyaul Islam,

Supurna Dhar

и другие.

Indian Journal of Tuberculosis, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Enhancing antibiotic therapy through comprehensive pharmacokinetic/pharmacodynamic principles DOI Creative Commons
Mohammad Sina Alikhani, Mohsen Nazari,

Shima Hatamkhani

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Фев. 25, 2025

Antibiotic therapy relies on understanding both pharmacokinetics (PK) and pharmacodynamics (PD), which respectively address drug absorption, distribution, elimination, the relationship between concentration antimicrobial efficacy. This review synthesizes decades of research, drawing from in-vitro studies, in-vivo models, clinical observations, to elucidate temporal dynamics antibiotic activity. We explore how these dynamics, including concentration-effect relationships post effects, inform classification antibiotics based their PD profiles. Additionally, we discuss pivotal role PK/PD principles in determining optimal dosage regimens. By providing a comprehensive overview therapy, this aims enhance improve treatment outcomes practice.

Язык: Английский

Процитировано

0
Recent progress in synthetic strategies for novel β-lactams linked with five-membered heterocycles (N/O/S): advances in medicinal chemistry (2020–2025) DOI

Ankita Garg,

Dolar Dureja,

Raaina Pasricha

и другие.

Medicinal Chemistry Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 12, 2025

Язык: Английский

Процитировано

0
Extended-Spectrum Beta-Lactamases (ESBLs) Gene Mutations in Kuwait: How Much Do We Know? Not Much! DOI Creative Commons

AA Dashti,

Mehrez M. Jadaon

Bacteria, Год журнала: 2025, Номер 4(2), С. 22 - 22

Опубликована: Апрель 30, 2025

Antibiotic resistance is a major problem worldwide, especially with the overuse and misuse of antibiotics. This makes it more challenging to treat patients infected antibiotic-resistant bacteria, costs on health system. review article describes extended-spectrum β-lactam antibiotics as most used antibacterial agents, how bacteria developed beta-lactamases (ESBLs) resist these The focuses ESBLs in Kuwait uncover which are present what ESBL gene mutations have been found this country. literature review, surprisingly, revealed limited number studies mutations, published over last 25 years. results showed that has an alarming bacterial strains mutations. These reported different isolated from types specimens, were collected hospitals Kuwait. data scattered not linked or analysed together highlight big picture highlights amount research up expectations country like Kuwait, high-standard facilities. Bearing mind geographic area relatively small, authors paper think there might be spreading need explored, collaboration needed among researchers address risky situation.

Язык: Английский

Процитировано

0
Detection of Oral Beta-Lactam Antibiotics Using a Taste Sensor with Surface-Modified Lipid/Polymer Membranes DOI Creative Commons
Takahiro Uchida, Ziyi Jiang, Zeyu Zhao

и другие.

Chemosensors, Год журнала: 2025, Номер 13(5), С. 186 - 186

Опубликована: Май 16, 2025

In our previous study, a taste sensor modified with 3-bromo-2,6-dihydroxybenzoic acid (3-Br-2,6-DHBA) exhibited significant responses to xanthine-based substances, suggesting an allosteric detection mechanism. This study investigates the potential of 3-Br-2,6-DHBA-modified membrane for detecting other drug classes. Eleven structurally diverse drugs—including caffeine, antibiotics, antivirals, analgesic-antipyretics from WHO Model List Essential Medicines Children—were tested, as they were previously undetectable by conventional bitterness sensor. Among them, amoxicillin, oral broad-spectrum penicillin, and cefalexin, cephalosporin, elicited significantly higher when was used. To further examine this response, experiments conducted using membranes 3-Br-2,6-DHBA, 2,6-dihydroxybenzoic (2,6-DHBA), benzoic acid. These tests confirmed that only produced amoxicillin hydroxyl groups in 3-Br-2,6-DHBA contribute effects via hydrogen bonding. Additional demonstrated cefaclor cefdinir, both cephalosporins. The interaction between beta-lactam ring, well adjacent five- or six-membered rings several cephalosporins, likely enables stacking π electron, hydrophobilc interaction, conclusion, effectively detects cephalosporins

Язык: Английский

Процитировано

0
Natural detoxification of antibiotics in the environment: A one health perspective DOI Creative Commons
Fernando Baquero, Teresa M. Coque, José Luis Martínez

и другие.

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Ноя. 24, 2022

The extended concept of one health integrates biological, geological, and chemical (bio-geo-chemical) components. Anthropogenic antibiotics are constantly increasingly released into the soil water environments. fate these drugs in thin Earth space (“critical zone”) where biosphere is placed determines effect antimicrobial agents on microbiosphere, which can potentially alter composition ecosystem lead to selection antibiotic-resistant microorganisms including animal human pathogens. However, environments highly heterogeneous their local composition; thus permanence activity antibiotics. This a case “molecular ecology”: antibiotic molecules adsorbed eventually inactivated by interacting with biotic abiotic that present at different concentrations places. There poorly explored aspects pharmacodynamics (PD, biological action) pharmacokinetics (PK, rates decay) In this review, we explore various factors contributing detoxification environment. These range from spontaneous degradation detoxifying effects produced clay minerals (forming geochemical platforms degradative reactions influenced light, metals, or pH), charcoal, natural organic matter (including cellulose chitin), biodegradation bacterial populations complex consortia “bacterial subsistence”; other words, microbes taking as nutrients), planktonic microalgae, fungi, plant removal degradation, sequestration living dead cells (necrobiome detoxification). Many processes occur particulated material bacteria origins (microbiota coalescence) might also attach (microbiotic particles), thereby determining environmental PK/PD influencing resistant bacteria. exploration field requires multidisciplinary effort developing molecular ecology antibiotics, but could result much more precise determination hazards production release.

Язык: Английский

Процитировано

14
A large screen identifies beta-lactam antibiotics which can be repurposed to target the syphilis agent DOI Creative Commons
K. Hayes, Jules M. Dressler, Steven J. Norris

и другие.

npj Antimicrobials and Resistance, Год журнала: 2023, Номер 1(1)

Опубликована: Июнь 1, 2023

Abstract Syphilis, caused by the spirochete Treponema pallidum subsp. (hereafter called T. ), is re-emerging as a worldwide sexually transmitted infection. A single intramuscular dose of benzathine penicillin G preferred syphilis treatment option. Both supply shortage concerns and potential for acquired antibiotic resistance further need to broaden repertoire therapeutics. We reasoned that other β-lactams may be equally or more effective at targeting disease-causing agent, , but have yet discovered due previous lack continuous in vitro culture system. Recent technical advances with respect propagation allowed us conduct high-throughput screen almost 100 β-lactams. Using several molecular cellular approaches we developed adapted, identified confirmed efficacy were similar outperformed current standard, G. These options are either currently used treat bacterial infections synthetic derivatives naturally occurring compounds. Our studies not only additional therapeutics resolution syphilis, provide techniques study complex biology —a has plagued human health centuries.

Язык: Английский

Процитировано

7