Journal of Eukaryotic Microbiology,
Год журнала:
2025,
Номер
72(3)
Опубликована: Май 1, 2025
ABSTRACT
The
topology
and
connectivity
of
rivers
can
be
modified
by
the
water
project
operation
for
agriculture
civilization,
as
exemplified
trellised
river
system
in
Chongming
Island.
Ciliates,
an
exceptional
indicator,
are
often
employed
monitoring
health
aquatic
ecosystems.
However,
assembly
structure
planktonic
microeukaryote
(e.g.,
ciliates)
communities,
especially
complex
networks,
rarely
investigated.
Here,
we
investigated
ciliate
community
mechanism
among
seasons
Island's
network
using
both
metabarcoding
quantitative
protargol
stain
(QPS)
approaches.
Both
methods
showed
that
ciliates
exhibited
similar
composition
at
class
level
distinct
seasonal
succession,
which
attributed
to
variations
nutrients,
dissolved
oxygen
temperature.
stochastic
process
was
main
factor
explaining
all
seasons,
is
probably
high
frequent
opening
sluices
operation.
Collectively,
unique
structure,
variation,
specific
driven
hydrology
were
reported
this
study.
Furthermore,
methodological
differences
also
should
fully
considered
when
conducting
ecological
study
hydrographic
waters.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 28, 2025
Pseudomonas
syringae
pv.
tomato
DC3000
(
Pst
DC3000)
is
a
plant
pathogenic
bacterium
that
possesses
complicated
motility
regulation
pathways
including
typical
chemotaxis
system.
A
significant
portion
of
our
understanding
about
the
genes
functioning
in
based
on
comparison
to
other
bacteria.
This
leaves
uncertainty
whether
gene
functions
are
conserved,
especially
since
specific
regulatory
modules
can
have
opposite
sets
.
In
this
study,
we
used
competitive
selection
enrich
for
mutants
with
altered
swimming
and
random
barcode
transposon-site
sequencing
(RB-TnSeq)
identify
roles
motility.
Besides
many
known
or
predicted
genes,
method
identified
PSPTO_0406
dipA
),
PSPTO_1042
chrR
)
PSPTO_4229
(hypothetical
protein)
as
novel
regulators.
homolog
,
cyclic
di-GMP
degrading
enzyme
P.
aeruginosa
part
an
extracytoplasmic
sensing
system
controls
expression
response
reactive
oxygen
species,
suggesting
may
function
mechanism
enables
alter
when
encountering
oxidative
stressors.
encodes
protein
containing
HD-related
output
domain
(HDOD),
but
no
previously
functions.
We
found
deletion
overexpression
both
reduce
motility,
its
sensitive
level.
phenotype
screen
nonsense
missense
longer
pair
conserved
arginine
residues
necessary
suppression.
Together
these
results
provide
global
perspective
structural
controlling
flagellar
DC3000.
Pathogens,
Год журнала:
2025,
Номер
14(3), С. 293 - 293
Опубликована: Март 18, 2025
The
human
microbiome
plays
a
vital
role
in
maintaining
homeostasis,
acting
as
key
regulator
of
host
immunity
and
defense
mechanisms.
However,
dysbiotic
microbial
communities
may
cause
disruption
the
symbiotic
relationship
between
local
microbiota,
leading
to
pathogenesis
various
diseases,
including
viral
infections
cancers.
One
most
common
infectious
agents
causing
cancer
is
papilloma
virus
(HPV),
which
accounts
for
more
than
90%
cervical
In
cases,
immune
system
activated
clears
HPV,
whereas
some
infection
persists
can
lead
precancerous
lesions.
Over
last
two
decades,
advent
next-generation
sequencing
(NGS)
technology
bioinformatics
has
allowed
thorough
in-depth
analysis
composition
anatomical
niches,
allowing
researchers
unveil
interactions
underlying
mechanisms
through
microbiota
could
affect
HPV
establishment,
persistence,
progression.
Accordingly,
present
narrative
review
aims
shed
light
on
our
understanding
context
its
progression,
mainly
cancer.
Furthermore,
we
explore
by
balance
exert
potential
pathogenic
or
protective
effects,
either
persistence
disease
outcomes
clearance.
Special
interest
given
how
modulate
infection.
Lastly,
summarize
latest
findings
therapeutic
efficacy
probiotics
prebiotics
preventing
and/or
treating
vaginal
transplantation
while
highlighting
significance
personalized
medicine
approaches
emerging
from
NGS-based
profiling
artificial
intelligence
(AI)
optimal
management
HPV-related
diseases.
Journal of Eukaryotic Microbiology,
Год журнала:
2025,
Номер
72(3)
Опубликована: Май 1, 2025
ABSTRACT
The
topology
and
connectivity
of
rivers
can
be
modified
by
the
water
project
operation
for
agriculture
civilization,
as
exemplified
trellised
river
system
in
Chongming
Island.
Ciliates,
an
exceptional
indicator,
are
often
employed
monitoring
health
aquatic
ecosystems.
However,
assembly
structure
planktonic
microeukaryote
(e.g.,
ciliates)
communities,
especially
complex
networks,
rarely
investigated.
Here,
we
investigated
ciliate
community
mechanism
among
seasons
Island's
network
using
both
metabarcoding
quantitative
protargol
stain
(QPS)
approaches.
Both
methods
showed
that
ciliates
exhibited
similar
composition
at
class
level
distinct
seasonal
succession,
which
attributed
to
variations
nutrients,
dissolved
oxygen
temperature.
stochastic
process
was
main
factor
explaining
all
seasons,
is
probably
high
frequent
opening
sluices
operation.
Collectively,
unique
structure,
variation,
specific
driven
hydrology
were
reported
this
study.
Furthermore,
methodological
differences
also
should
fully
considered
when
conducting
ecological
study
hydrographic
waters.
