Microbiota signature of the lung as the promising bioindicator for drowning diagnosis and postmortem submersion interval estimation

International Journal of Legal Medicine, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Язык: Английский

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Metabarcoding for the Monitoring of the Microbiome and Parasitome of Medically Important Mosquito Species in Two Urban and Semi-urban Areas of South Korea

Current Microbiology, Год журнала: 2025, Номер 82(3)

Опубликована: Янв. 26, 2025

Язык: Английский

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Optimization of Fermented Maize Stover for the Fattening Phase of Geese: Effect on Production Performance and Gut Microflora

Animals, Год журнала: 2024, Номер 14(3), С. 433 - 433

Опубликована: Янв. 29, 2024

To optimize the utilization of fermented maize stover (FMS) feed during fattening phase Xianghai flying geese (XFG), a total 300 XFG at 125 days age were randomly assigned to four dietary treatment groups with three replicates 25 in each set. Group A was fed basal diet, while B, C, and D basic diet diets supplemented 5%, 10% or 15% FMS, respectively. The findings indicate that production performance indicators (especially dressed, eviscerated breast muscle yield) closely resembled more than Groups B C. Intestinal morphometry found jejunal villus height height/crypt depth significantly increased compared A. Next, 16S rRNA amplicon sequencing extracted DNA revealed beneficial microbiota (Coprococcus Victivallis) showed abundance D. Cecal flora function analysis further some amino acid glycerol biosynthesis be associated growth geese. These suggest incorporating FMS as substitute for portion can effectively sustain their performance, gut microbial community morphometrical traits, provide new insight into using non-conventional resources reduce cost improve economic benefits breeding industry.

Язык: Английский

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Multi-label classification to predict antibiotic resistance from raw clinical MALDI-TOF mass spectrometry data

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Дек. 28, 2024

Abstract Antimicrobial resistance (AMR) poses a significant global health challenge, necessitating advanced predictive models to support clinical decision-making. In this study, we explore multi-label classification as novel approach predict antibiotic across four clinically relevant bacteria: E. coli , S. aureus K. pneumoniae and P. aeruginosa . Using multiple datasets from the DRIAMS repository, evaluated performance of algorithms – Multi-Layer Perceptron, Support Vector Classifier, Random Forest, Extreme Gradient Boosting under both single-label frameworks. Our results demonstrate that delivers competitive compared traditional models, with no statistically differences in most cases. The framework naturally captures complex, interconnected nature AMR data, reflecting real-world scenarios more accurately. We further validated on external (DRIAMS B C), confirming their generalizability robustness. Additionally, investigated impact oversampling techniques provided reproducible methodology for handling MALDI-TOF ensuring scalability future studies. These findings underscore potential enhance accuracy research, offering valuable insights developing diagnostic tools guiding interventions.

Язык: Английский

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Postbiotics in the Bakery Products: Applications and Nutritional Values

Probiotics and Antimicrobial Proteins, Год журнала: 2024, Номер unknown

Опубликована: Июль 27, 2024

Язык: Английский

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Action mechanism of the potential biocontrol agent Brevibacillus laterosporus SN19-1 against Xanthomonas oryzae pv. oryzae causing rice bacterial leaf blight

Archives of Microbiology, Год журнала: 2023, Номер 206(1)

Опубликована: Дек. 24, 2023

Язык: Английский

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From the perspective of rumen microbiome and host metabolome, revealing the effects of feeding strategies on Jersey Cows on the Tibetan Plateau

PeerJ, Год журнала: 2023, Номер 11, С. e16010 - e16010

Опубликована: Сен. 11, 2023

Previous studies have discussed the effects of grazing and house feeding on yaks during cold season when forage is in short supply, but there limited information these strategies Jersey cows introduced to Tibetan Plateau. The objective this study was use genomics metabolomics analyses examine changes rumen microbiology organism metabolism with different strategies.We selected 12 similar body conditions kept them for 60 days under (n = 6) house-feeding conditions. At end experiment, samples fluid serum were collected from that had been fed using strategies. analyzed fermentation parameters, bacterial communities, antioxidant immunological indices, metabolomics. results examined find appropriate plateau.The parameters showed concentrations acetic acid, propionic ammonia nitrogen group (Group B) significantly higher than G) (P < 0.05). In terms community 16S rRNA gene, Chao1 index Group G B 0.038), while observed species, Shannon Simpson indices not above-mentioned groups > Beta diversity analysis revealed no significant differences composition microbiota between two groups. Analysis immune total capacity 0.05), IL-6, Ig-M , TNF-α LC-MS a 149 major differential metabolites found B. enriched metabolic pathways biosynthesis amino acids, protein digestion absorption, ABC transporters, aminoacyl-tRNA biosynthesis, mineral unsaturated fatty acids. These data suggest strategy more beneficial improve physiological state Plateau forages are supply.

Язык: Английский

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chem16S: community-level chemical metrics for exploring genomic adaptation to environments

Bioinformatics, Год журнала: 2023, Номер 39(9)

Опубликована: Сен. 1, 2023

The chem16S package combines taxonomic classifications of 16S rRNA gene sequences with amino acid compositions prokaryotic reference proteomes to generate community proteomes. Taxonomic from the RDP Classifier or data objects created by phyloseq R are supported. Users can calculate and visualize a variety chemical metrics in order explore effects redox, salinity, other physicochemical variables on genomic adaptation protein at level.Development is hosted https://github.com/jedick/chem16S. Version 1.0.0 freely available Comprehensive Archive Network (CRAN) https://cran.r-project.org/package=chem16S.

Язык: Английский

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Prescribed burning alters soil microbial community structure by changing soil physicochemical properties in temperate forests of northern China

Journal of Forestry Research, Год журнала: 2024, Номер 35(1)

Опубликована: Окт. 12, 2024

Abstract Prescribed burning is commonly used to maintain forest ecosystem functions and reduce the risk of future wildfires. Although many studies have investigated response microbial community wildfires in ecosystems, effects prescribed burnings on soil structure are less studied. It also unclear that how post-fire physiochemical properties changes affected communities. Here, we studied impacts microbiome three typical temperate forests northern China by collecting physicochemical high-throughput sequencing for 16S rRNA 18S was applied analyze diversity composition microbes (bacteria fungi). Compared with pre-fire condition, significantly decreased Chao1 index altered bacterial communities ( P < 0.05), whereas it had no significant effect fungal > 0.05). Planctomycetes Actinobacteria made greatest contributions dissimilarity between conditions. The main variables influencing pH, available phosphorus, total nitrogen, ratio carbon which could account 73.5% variation these stands. Our findings demonstrated a great discrepancy responses bacteria fungi burning. modifying properties. results pointed essential evaluate impact functions. These provide an important baseline assessing recovery region offer critical guidance restoration efforts.

Язык: Английский

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Dietary Chlorella vulgaris supplementation modulates health, microbiota and the response to oxidative stress of Atlantic salmon

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Окт. 10, 2024

Microalgae are emerging as functional feed ingredients in aquaculture due to their immune-stimulating and stress-modulating properties. We investigated the potential of microalgae Chlorella vulgaris a supplement improve health modulate microbiota stress responses Atlantic salmon. Triplicate groups salmon (~ 126 g) were reared recirculating system (RAS) at 15 °C received diets supplemented with 2% (CV2) or 14% (CV14) spray-dried C. daily, once weekly (CV14w), control diet (CD) for 8 weeks. Subsequently, all exposed an acute one-hour peracetic acid (CH

Язык: Английский

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