Environmental Science & Technology,
Год журнала:
2023,
Номер
57(50), С. 21358 - 21369
Опубликована: Дек. 11, 2023
Though
the
evidence
for
antibiotic
resistance
spread
via
plant
microbiome
is
mounting,
studies
regarding
resistome
in
seed,
a
reproductive
organ
and
important
food
resource,
are
still
their
infancy.
This
study
investigated
effects
of
long-term
organic
fertilization
on
seed
bacterial
endophytes,
resistome,
intergenerational
transfer
microcosm.
A
total
99
genes
(ARGs)
26
mobile
genetic
elements
(MGEs)
were
detected
by
high-throughput
quantitative
PCR.
The
amount
fertilizer
applied
was
positively
correlated
to
number
relative
abundance
seed-associated
ARGs
MGEs.
Moreover,
transmission
from
rhizosphere
mainly
mediated
shared
bacteria
Notably,
progeny
seedlings
derived
seeds
harboring
abundant
found
have
higher
ARGs.
Using
structural
equation
models,
we
further
revealed
that
MGEs
key
factors
affecting
rhizosphere,
implying
potential
reservoir
soil.
highlights
overlooked
role
endophytes
dissemination
soil-plant
continuum,
more
attention
should
be
paid
as
vectors
within
"One-Health"
framework.
Abstract
Contemporary
poultry
farming
involves
extensive
antibiotic
use,
which
could
potentially
pose
health
risks
to
consumers
through
residues
in
animal-derived
food
products,
especially
meat.
Recent
decisions,
particularly
the
ban
on
nearly
all
feed
additives
utilized
as
growth
promoters,
have
resulted
a
decrease
their
usage.
Nonetheless,
essential
role
therapy
and
economic
value
are
indisputable.
This
study
evaluated
presence
of
marketed
turkey
meat
using
four-box
method.
The
analyses
indicated
that,
400
samples
examined,
overall
prevalence
contamination
was
65.75%.
Among
different
types
antibiotics
identified
samples,
β-lactam/tetracycline
were
highest,
with
41.44%.
analysis
sample
revealed
significant
rates
organs,
liver,
approximately
83.75%,
wing
muscle,
78.75%.
Two
families,
β-lactam
tetracycline,
muscle
liver
at
frequencies
44.44%
43.28%,
respectively.
Regarding
cross-contamination,
positive
exhibited
concurrently
by
specific
type
residue,
notable
rate
58.19%.
All
analyzed
organs
multiple
residue
types,
varying
contaminants
present
organs.
findings
detection
consumed
These
highlight
excessive
use
industry,
increasing
consumer
exposure
these
residues'associated
risks.
Therefore,
it
is
implement
stricter
measures
monitoring
systems
regarding
farming.
Biotechnology Journal,
Год журнала:
2025,
Номер
20(4)
Опубликована: Апрель 1, 2025
ABSTRACT
The
use
of
microorganisms
in
environmental
biotreatment
is
gaining
attention,
particularly
Bacillus
subtilis
(B.
subtilis)
,
recognized
for
its
effectiveness
wastewater
treatment
and
soil
remediation.
Its
success
stems
from
diverse
biological
activities
adaptability,
which
improve
quality
ecological
balance.
This
paper
reviews
the
remediation
capabilities
mechanisms
B.
focusing
on
applications
water
purification
pollution
management.
facilitates
pollutant
degradation
adsorption
through
enzyme
production,
organic
acids,
unique
cell
wall
properties,
interactions
with
other
microorganisms.
article
addresses
current
challenges
future
directions,
emphasizing
need
enhanced
cultivation,
screening,
genetic
engineering
functional
strains.
Understanding
these
strains
studying
their
toxicological
impacts
are
essential
optimizing
microbial
remediation,
providing
both
theoretical
practical
foundations
bioremediation
efforts.
Microbial Genomics,
Год журнала:
2025,
Номер
11(4)
Опубликована: Апрель 28, 2025
Microbial
biostimulants
(MBs)
offer
a
sustainable
approach
to
agriculture
by
helping
reduce
reliance
on
synthetic
fertilizers.
However,
as
MBs
are
intentionally
released
into
the
environment,
their
safety
should
be
rigorously
assessed.
While
taxa
with
qualified
presumption
of
(QPS)
benefit
from
established
indications,
non-QPS
lack
such
guidance.
To
address
this
gap,
we
propose
pipeline
combining
whole
genome
sequencing
(WGS)
and
extensive
literature
search
(ELS)
data
evaluate
microbial
safety.
We
analysed
public
genomes
three
QPS
species
(
Rhodopseudomonas
palustris
,
Bacillus
velezensis
Priestia
megaterium
)
four
genera
Arthrobacter
Azotobacter
Azospirillum
Herbaspirillum
),
screening
them
for
virulence
factors
(VFs),
antimicrobial
resistance
(AMR)
genes
mobile
genetic
elements
(MGEs).
Results
confirmed
taxa,
revealing
no
VFs
only
few
intrinsic
non-clinically
relevant
AMRs.
Among
VF
hits
were
more
prevalent
in
spp.,
though
they
mostly
related
beneficial
plant
interactions
rather
than
pathogenicity.
AMR
primarily
associated
efflux
pumps
or
sporadically
distributed.
Notably,
genus-wide
pattern
observed
was
that
most
harboured
chromosomally
encoded
β
-lactamases
sharing
similar
structures;
however,
detected
-lactamase
bla
distantly
clinically
variants,
absence
MGEs
suggests
low
risk
horizontal
gene
transfer,
indicating
overall
these
genera.
In
general,
WGS–ELS
framework
provides
robust
tool
assessing
MBs,
supporting
regulatory
decision-making
ensuring
safe
use
while
safeguarding
health.
