Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Апрель 20, 2023
Gymnastic
equipment
surfaces
are
shared
by
many
people,
and
could
mediate
the
transfer
of
bacterial
pathogens.
To
better
understand
this
detrimental
potential,
investigations
on
reservoirs
pathogens
antimicrobial
resistance
gymnastic
were
performed
analyzing
community
structures,
prevalence
viable
bacteria,
presence
both
indoor
outdoor
facilities.
The
results
high-throughput
16S
rDNA
amplicon
sequencing
showed
that
Gram-positive
bacteria
significantly
enriched,
including
opportunistic
pathogen
Staphylococcus
strains,
while
Enterobacteriaceae
enriched
equipment.
analysis
α-diversities
a
higher
richness
diversity
for
communities
than
environment.
Analysis
β-diversities
differ
from
environmental
communities,
also
different.
Thirty-four
isolates
obtained
equipment,
three
multidrug
one
resistant
Pantoea.
In
particular,
hemolyticus
5-6,
isolated
dumbbell
surface,
is
resistant,
hemolytic,
high-
risk
pathogen.
quantitative
PCR
targeting
antibiotic
related
genes
(intI1,
sul1
bla
TEM)
abundances
TEM
environment,
gene
These
lead
to
conclusion
potential
dissemination
pathways
highly
dangerous
as
well
resistance,
risks
Limnology and Oceanography,
Год журнала:
2024,
Номер
69(8), С. 1870 - 1881
Опубликована: Июль 21, 2024
Abstract
Wastewater
treatment
plant
effluents
release
microbiological
pollutants,
including
the
intI
1
gene
(integrases
of
class
integron),
which
has
been
proposed
as
a
target
for
monitoring
anthropogenic
pollution
in
surface
waters.
This
correlates
with
antibiotic
resistance
genes,
making
it
an
important
proxy
genetic
contamination
aquatic
environments.
It
is
currently
unclear
whether
found
lake
water
mainly
present
due
to
continuous
seeding
or
if
autochthonous
bacteria
harbor
this
gene.
To
better
understand
fate
and
dynamics
integrons
systems,
we
resorted
classical
limnological
(qPCR)
over
multiple
years
three
different
size
fractions:
free‐living
bacteria,
particle‐attached
zooplankton‐attached
bacteria.
We
also
conducted
experiments
elucidate
impact
grazers
on
abundance
1.
The
fractions
Lake
Maggiore
microbial
community
showed
particle‐bound
lifestyle
1‐hosting
Most
these
originated
from
both
wastewater
effluent
that
discharges
into
itself
(amplicon
sequencing).
hypothesize
grow
particles
open
waters,
them
particularly
vulnerable
grazing
by
large
filter
feeders
such
Daphnia
.
Therefore,
presence
reduced
water,
whereas
was
not
true
other
Rotaria
macrura
Poterioochromonas
sp.
Our
study
shows
food
web
structure
temporal
changes
influence
consequently
assessment
pollution.
The Science of The Total Environment,
Год журнала:
2024,
Номер
955, С. 176765 - 176765
Опубликована: Окт. 10, 2024
The
global
spread
of
antibiotic
resistance
genes
(ARGs)
in
the
marine
environment
poses
a
significant
threat
to
public
health
and
natural
ecosystems.
This
study
quantified
analysed
distribution
co-occurrence
patterns
ARGs
wide
range
oceans
high
seas,
including
Atlantic,
Arctic
Indian
Ocean,
Mediterranean
Sea
Persian
Gulf.
Focusing
on
beta-lactamases
(bla
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Апрель 20, 2023
Gymnastic
equipment
surfaces
are
shared
by
many
people,
and
could
mediate
the
transfer
of
bacterial
pathogens.
To
better
understand
this
detrimental
potential,
investigations
on
reservoirs
pathogens
antimicrobial
resistance
gymnastic
were
performed
analyzing
community
structures,
prevalence
viable
bacteria,
presence
both
indoor
outdoor
facilities.
The
results
high-throughput
16S
rDNA
amplicon
sequencing
showed
that
Gram-positive
bacteria
significantly
enriched,
including
opportunistic
pathogen
Staphylococcus
strains,
while
Enterobacteriaceae
enriched
equipment.
analysis
α-diversities
a
higher
richness
diversity
for
communities
than
environment.
Analysis
β-diversities
differ
from
environmental
communities,
also
different.
Thirty-four
isolates
obtained
equipment,
three
multidrug
one
resistant
Pantoea.
In
particular,
hemolyticus
5-6,
isolated
dumbbell
surface,
is
resistant,
hemolytic,
high-
risk
pathogen.
quantitative
PCR
targeting
antibiotic
related
genes
(intI1,
sul1
bla
TEM)
abundances
TEM
environment,
gene
These
lead
to
conclusion
potential
dissemination
pathways
highly
dangerous
as
well
resistance,
risks
