Growth balance analysis models of cyanobacteria for understanding resource allocation strategies DOI Creative Commons
Sajjad Ghaffarinasab, Martin J. Lercher, Hugo Dourado

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Апрель 18, 2023

Abstract Cyanobacteria have emerged as attractive microbial cell factories, they can convert atmospheric CO 2 and sunlight into valuable chemicals. To increase their growth productivity, one should aim to optimize the allocation of limited cellular resources across different metabolic processes. Here, we developed two balance analysis (GBA) models for cyanobacterium Synechocystis sp. PCC 6803. In its biological assumptions, closely related an existing coarse-grained model, while mathematical formulation is heavily streamlined. We show that GBA provide virtually identical predictions about resource among photosynthesis, carbon metabolism, protein translation machinery under environmental conditions previous, mathematically more involved model. Our model also captures effects photodamage on proteome resulting rates. further how be easily extended include reactions, leading a second capable new allocation. Balanced type presented here expanded details, providing useful toolbox understanding physiological capabilities cyanobacteria, resources, potential bioengineering optimized biomass production.

Язык: Английский

Genome streamlining to improve performance of a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 DOI Creative Commons
Annesha Sengupta, Anindita Bandyopadhyay, Debolina Sarkar

и другие.

mBio, Год журнала: 2024, Номер 15(3)

Опубликована: Фев. 15, 2024

ABSTRACT Cyanobacteria are photosynthetic organisms that have garnered significant recognition as potential hosts for sustainable bioproduction. However, their complex regulatory networks pose challenges to major metabolic engineering efforts, thereby limiting feasibility production hosts. Genome streamlining has been demonstrated be a successful approach improving productivity and fitness in heterotrophs but is yet explored its full phototrophs. Here, we present the systematic reduction of genome cyanobacterium exhibiting fastest exponential growth, Synechococcus elongatus UTEX 2973. This work, first kind photoautotroph, involved an iterative process using state-of-the-art genome-editing technology guided by experimental analysis computational tools. CRISPR-Cas3 enabled large, progressive deletions predicted dispensable regions aided identification essential genes. The large were combined obtain strain with 55-kb reduction. strains streamlined showed improvement growth (up 23%) (by 22.7%) compared wild type (WT). strategy not only develop cyanobacterial improved traits can also facilitate better understanding genome-to-phenome relationships. IMPORTANCE evolutionary used natural living systems dispense unnecessary genes from mechanism adapt evolve. While this successfully borrowed synthetic heterotrophic microbial desired phenotype, it extensively photoautotrophs. incorporates both predictions identify validation tool, study, employed modified goal minimize size extent allows optimal cellular under specified conditions. Our novel tool photoautotrophs, which, unlike other existing tools, enables spontaneous genome. findings demonstrate effectiveness obtaining genome, productivity.

Язык: Английский

Процитировано

10
Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation DOI Creative Commons

A. Gilliam,

Natalie Sadler, Xiaolu Li

и другие.

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Март 8, 2025

Abstract Background The industrial feasibility of photosynthetic bioproduction using cyanobacterial platforms remains challenging due to insufficient yields, particularly competition between product formation and cellular carbon demands across different temporal phases growth. This study investigates how circadian clock regulation impacts partitioning storage, growth, synthesis in Synechococcus elongatus PCC 7942, provides insights that suggest potential strategies for enhanced bioproduction. Results After entrainment light-dark cycles, 7942 cultures transitioned constant light revealed distinct patterns sucrose production, exhibiting three-fold higher productivity during subjective night compared day despite moderate down-regulation genes from the apparatus. coincided with reduced glycogen accumulation halted cell division at time, suggesting separation competing processes. Transcriptome analysis coordinated clock-driven adjustment cycle rewiring energy metabolism, over 300 showing differential expression four time points. was characterized by altered division-related involved synthesis, while upregulation degradation pathways, alternative electron flow components, pentose phosphate pathway, oxidative decarboxylation pyruvate. These molecular changes created favorable conditions through availability major precursors (glucose-1-phosphate fructose-6-phosphate) maintained redox balance multiple mechanisms. Conclusions Our regulatory metabolism balancing suggests two approaches could be developed improving bioproduction: leveraging natural rhythms optimizing cultivation timing pathway induction, engineering strains mimic circadian-driven metabolic shifts controlled flux redistribution rebalancing. While these remain tested, they theoretically improve efficiency enabling better storage accumulation, phases.

Язык: Английский

Процитировано

1
Light-driven biosynthesis of volatile, unstable and photosensitive chemicals from CO2 DOI Open Access
Chaofeng Li,

Yin Lijie,

Jiawei Wang

и другие.

Nature Synthesis, Год журнала: 2023, Номер 2(10), С. 960 - 971

Опубликована: Май 25, 2023

Язык: Английский

Процитировано

17
Polyhydroxyalkanoates bioproduction from bench to industry: Thirty years of development towards sustainability DOI
Priskila Adjani Diankristanti, Yu‐Chieh Lin, Ying-Chen Yi

и другие.

Bioresource Technology, Год журнала: 2023, Номер 393, С. 130149 - 130149

Опубликована: Дек. 2, 2023

Язык: Английский

Процитировано

15
Biomedical engineering utilizing living photosynthetic cyanobacteria and microalgae: Current status and future prospects DOI Creative Commons
Tong Zhang, Dailin Liu, Yingying Zhang

и другие.

Materials Today Bio, Год журнала: 2024, Номер 27, С. 101154 - 101154

Опубликована: Июль 14, 2024

Cyanobacteria are the only prokaryotes capable of performing oxygenic photosynthesis on Earth. Besides their traditional roles serving as primary producers, cyanobacteria also synthesize abundant secondary metabolites including carotenoids, alkaloids, peptides, which have been reported to possess medicinal potentials. More importantly, advancement synthetic biology technology has further expanded potential biomedical applications especially using living/engineered cyanobacteria, providing promising and attractive strategies for future disease treatments. To improve understanding facilitate applications, this review aims discuss current status prospects cyanobacterial-based engineering. Firstly, specific properties related with like natural products bioactive compounds heavy metal adsorption were concluded. Subsequently, based these we discussed progress in various models hypoxia microenvironment alleviation, wound healing, drug delivery, so on. Finally, exploration metabolites, integration synthesized by situ medical diagnosis treatment, optimization vivo application critically presented. The will promote studies cyanobacteria-based engineering its practical clinical trials future.

Язык: Английский

Процитировано

4
Exploring the accumulation of Poly-β-hydroxybutyrate in the marine cyanobacterium Nostoc sp. BDU 00591: A comprehensive study on extraction, stimulation and characterisation DOI

P. Anjali,

Sudatta Maity, Nirupama Mallick

и другие.

Biocatalysis and Agricultural Biotechnology, Год журнала: 2025, Номер unknown, С. 103488 - 103488

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Glucose 6-phosphate enrichment augments AGPase enzyme activity and biomass production in the cyanobacterium Nostoc muscorum Meg 1 DOI Creative Commons

Lanakadaphi R. Chullai,

Balakyntiewshisha Lyngdoh Kynshi, Mayashree B. Syiem

и другие.

Journal of Applied Phycology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

Язык: Английский

Процитировано

0
CyanoStat: An open-source platform for CO2 regulation in microbial incubators optimized for cyanobacterial cultivation DOI Creative Commons
Tijn O. Delzenne, Dennis Claessen

HardwareX, Год журнала: 2025, Номер unknown, С. e00649 - e00649

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Biology and composition of microalgae and cyanobacteria DOI

Diana Rodríguez-Zúñiga,

Alejandro Méndez‐Zavala,

Orlando Solís-Quiroz

и другие.

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 1 - 22

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

3
Strong heterologous electron sink outcompetes alternative electron transport pathways in photosynthesis DOI Creative Commons
Michal Hubáček, Laura T. Wey, Robert Kourist

и другие.

The Plant Journal, Год журнала: 2024, Номер 119(5), С. 2500 - 2513

Опубликована: Июль 15, 2024

SUMMARY Improvement of photosynthesis requires a thorough understanding electron partitioning under both natural and strong sink conditions. We applied wide array state‐of‐the‐art biophysical biochemical techniques to thoroughly investigate the fate photosynthetic electrons in engineered cyanobacterium Synechocystis sp. PCC 6803, blueprint for biotechnology, expressing heterologous gene ene‐reductase, YqjM. This recombinant enzyme catalyses reduction an exogenously added substrate into desired product by utilising photosynthetically produced NAD(P)H, enabling whole‐cell biotransformation. Through coupling biotransformation reaction with measurements, we demonstrated that artificial sink, outcompetes valves, flavodiiron protein‐driven Mehler‐like cyclic transport. These results show ferredoxin‐NAD(P)H‐oxidoreductase is preferred route delivering from reduced ferredoxin cellular NADPH/NADP+ ratio as key factor orchestrating flux. insights are crucial molecular mechanisms transport harnessing sustainable bioproduction engineering source/sink balance. Furthermore, conclude identifying bioenergetic bottleneck prerequisite targeted platforms.

Язык: Английский

Процитировано

2