bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 18, 2023
Abstract
Cyanobacteria
have
emerged
as
attractive
microbial
cell
factories,
they
can
convert
atmospheric
CO
2
and
sunlight
into
valuable
chemicals.
To
increase
their
growth
productivity,
one
should
aim
to
optimize
the
allocation
of
limited
cellular
resources
across
different
metabolic
processes.
Here,
we
developed
two
balance
analysis
(GBA)
models
for
cyanobacterium
Synechocystis
sp.
PCC
6803.
In
its
biological
assumptions,
closely
related
an
existing
coarse-grained
model,
while
mathematical
formulation
is
heavily
streamlined.
We
show
that
GBA
provide
virtually
identical
predictions
about
resource
among
photosynthesis,
carbon
metabolism,
protein
translation
machinery
under
environmental
conditions
previous,
mathematically
more
involved
model.
Our
model
also
captures
effects
photodamage
on
proteome
resulting
rates.
further
how
be
easily
extended
include
reactions,
leading
a
second
capable
new
allocation.
Balanced
type
presented
here
expanded
details,
providing
useful
toolbox
understanding
physiological
capabilities
cyanobacteria,
resources,
potential
bioengineering
optimized
biomass
production.
Bioresource Technology,
Год журнала:
2024,
Номер
410, С. 131232 - 131232
Опубликована: Авг. 6, 2024
Applying
low-cost
substrate
is
critical
for
sustainable
bioproduction.
Co-culture
of
phototrophic
and
heterotrophic
microorganisms
can
be
a
promising
solution
as
they
use
CO
Antimicrobial Agents and Chemotherapy,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 12, 2024
ABSTRACT
Metformin,
a
safe
biguanide
derivative
with
antiproliferative
properties,
has
shown
antiparasitic
efficacy
against
the
Echinococcus
larval
stage.
Hence,
we
assessed
of
dose
250
mg
kg
−1
day
in
experimental
models
advanced
CE,
at
6
and
12
months
post-infection
oral
intraperitoneal
administration,
respectively.
At
this
high
dose,
metformin
reached
intracystic
concentrations
between
0.7
1.7
mM
triggered
Eg-TOR
inhibition
through
AMPK
activation
by
AMP-independent
-dependent
mechanisms,
which
are
dependent
on
drug
dose.
Cystic
uptake
was
controlled
increased
expression
organic
cation
transporters
presence
drug.
In
both
models,
reduced
weight
parasite
cysts,
altered
ultrastructural
integrity
their
germinal
layers,
availability
glucose,
limiting
cellular
carbon
energy
charge
proliferative
capacity
metacestodes.
This
glucose
depletion
associated
slight
increase
cystic
2-deoxiglucose
transcriptional
induction
GLUT
genes
context,
drastic
glycogen
consumption
led
to
lactate
production
intermediary
metabolism
treated
Specifically,
fraction
reducing
soluble
sugars
decreased
twofold,
levels
non-reducing
sugars,
such
as
sucrose
trehalose,
were
modified
fluid
cells.
Taken
together,
our
findings
highlight
relevance
promising
candidate
for
CE
treatment
warrant
further
research
improve
therapeutic
conditions
chronic
zoonosis
humans.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 20, 2024
Abstract
Improvement
of
photosynthesis
requires
a
thorough
understanding
electron
partitioning
under
both
natural
and
strong
sink
conditions.
We
applied
wide
array
state-of-the-art
biophysical
biochemical
techniques
to
thoroughly
investigate
the
fate
photosynthetic
electrons
in
engineered
cyanobacterium
Synechocystis
sp.
PCC
6803,
blueprint
for
biotechnology,
expressing
heterologous
gene
ene-reductase,
YqjM.
This
recombinant
enzyme
catalyses
reduction
an
exogenously
added
substrate
into
desired
product
by
utilising
photosynthetically
produced
NAD(P)H,
enabling
whole-cell
biotransformation.
Through
coupling
biotransformation
reaction
with
measurements,
we
demonstrated
that
artificial
sink,
outcompetes
valves,
flavodiiron
protein-driven
Mehler-like
reaction,
cyclic
transport.
These
results
show
ferredoxin-NAD(P)H-oxidoreductase
(FNR)
is
preferred
route
delivering
from
reduced
ferredoxin
cellular
NADPH/NADP+
ratio
as
key
factor
orchestrating
flux.
insights
are
crucial
molecular
mechanisms
transport
harnessing
sustainable
bioproduction
engineering
source/sink
balance.
Furthermore,
conclude
identifying
bioenergetic
bottleneck
prerequisite
targeted
platforms.
Significance
statement
coupled
biocatalytic
(whole-cell
biotransformation)
performance
model
cyanobacteria.
employed
NAD(P)H
enzyme,
allowing
us
gain
comprehensive
partitioning.
sinks
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Апрель 8, 2024
Cyanobacteria
have
been
proposed
as
a
potential
alternative
carbohydrate
feedstock
and
multiple
species
successfully
engineered
to
secrete
fermentable
sugars.
To
date,
the
most
productive
cyanobacterial
strains
are
those
designed
sucrose,
yet
there
exist
considerable
differences
in
reported
productivities
across
different
model
laboratories.
In
this
study,
we
investigate
how
cultivation
conditions
(specifically,
irradiance,
CO
2
,
cultivator
type)
affect
productivity
of
sucrose-secreting
Synechococcus
elongatus
PCC
7942.
We
find
that
S.
produces
highest
sucrose
yield
irradiances
far
greater
than
what
is
often
experimentally
utilized,
high
light
intensities
tolerated
by
especially
under
higher
density
where
turbidity
may
attenuate
effective
experienced
culture.
By
increasing
inorganic
carbon
availability,
cscB
/
sps
produced
total
3.8
g
L
-1
within
period
being
47.8
mg
h
.
This
study
provides
quantitative
description
impact
culture
on
cyanobacteria-derived
assist
standardize
cross-laboratory
comparisons
demonstrates
significant
capacity
improve
via
optimizing
conditions.
Microbial Biotechnology,
Год журнала:
2024,
Номер
17(8)
Опубликована: Авг. 1, 2024
The
latest
assessment
of
progress
towards
the
Sustainable
Development
Goals
(SDGs)
has
identified
major
obstacles,
such
as
climate
change,
global
instability
and
pandemics,
which
threaten
efforts
to
achieve
SDGs
even
by
2050.
Urgent
action
is
needed,
particularly
reduce
poverty,
hunger
change.
In
this
context,
microalgae
are
emerging
a
promising
solution,
in
context
food
security
environmental
sustainability.
As
versatile
organisms,
offer
nutritional
benefits
high-quality
proteins
essential
fatty
acids,
can
be
cultivated
non-arable
areas,
reducing
competition
for
resources
improving
sustainability
systems.
role
also
includes
other
applications
aquaculture,
where
they
serve
sustainable
alternatives
animal
feed,
agriculture,
act
biofertilizers
biostimulants.
These
microorganisms
play
key
interventions
on
degraded
land,
stabilizing
soils,
hydrological
function
increasing
nutrient
carbon
availability.
Microalgae
therefore
support
several
promoting
agricultural
practices
contributing
land
restoration
sequestration
efforts.
integration
these
areas
mitigate
impacts
improve
security,
highlighting
need
increased
research
development,
well
public
political
support,
exploit
their
full
potential
advance
SDGs.
Synthetic
light-driven
consortia
composed
of
phototrophs
and
heterotrophs
have
attracted
increasing
attention
owing
to
their
potential
be
used
in
sustainable
biotechnology.
In
recent
years,
synthetic
phototrophic
been
produce
bulk
chemicals,
biofuels,
other
valuable
bioproducts.
addition,
autotrophic-heterotrophic
symbiosis
systems
applications
wastewater
treatment,
bioremediation,
as
a
method
for
phytoplankton
bloom
control.
Here,
we
discuss
progress
made
on
the
biosynthesis
microbial
consortia.
strategies
optimizing
are
summarized.
Moreover,
highlight
current
challenges
future
research
directions
development
robust
controllable
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 16, 2024
Cyanobacteria
are
photosynthetic
organisms
that
have
garnered
significant
recognition
as
potential
hosts
for
sustainable
bioproduction.
However,
their
complex
regulatory
networks
pose
challenges
to
major
metabolic
engineering
efforts,
thereby
limiting
feasibility
production
hosts.
Genome
streamlining
has
been
demonstrated
be
a
successful
approach
improving
productivity
and
fitness
in
heterotrophs
but
is
yet
explored
its
full
phototrophs.
Here
we
present
the
systematic
reduction
of
genome
cyanobacterium
exhibiting
fastest
exponential
growth,
Synechococcus
elongatus
UTEX
2973.
This
work,
first
kind
photoautotroph,
involved
an
iterative
process
using
state-of-the-art
genome-editing
technology
guided
by
experimental
analysis
computational
tools.
CRISPR/Cas3
enabled
large,
progressive
deletions
predicted
dispensable
regions
aided
identification
essential
genes.
The
large
were
combined
obtain
strain
with
55
kb
reduction.
strains
streamlined
showed
improvement
growth
(up
23%)
(by
22.7%)
compared
WT.
strategy
not
only
develop
cyanobacterial
improved
traits
can
also
facilitate
better
understanding
phenome
relationships.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Март 19, 2024
Introduction
Cyanobacteria
are
typically
of
a
size
that
can
be
observed
under
microscope.
Here,
we
present
cyanobacteria
with
the
naked
eye.
Arthrospira
platensis
NCB002
strain
showed
differentiated
morphological
characteristics
compared
to
previously
reported
spp.
Methods
was
obtained
by
UV
irradiation
sp.
NCB001,
which
isolated
from
freshwater
and
owned
NCell
Co.,
Ltd.
A.
NIES-39
National
Institute
for
Environmental
Studies
(Tsukuba,
Japan).
We
used
various
analytical
techniques
determine
its
overall
characteristics.
Results
discussion
The
draft
genome
consists
five
contigs
comprising
6,864,973
bp
G+C
content
44.3
mol%.
had
an
average
length
11.69
±
1.35
mm
maximum
15.15
mm,
is
23.4–50.5
times
longer
than
(0.3–0.5
mm)
known
spp.,
allowing
it
harvested
using
thin
sieve.
Transcriptome
analysis
revealed
these
differences
resulted
changes
in
cell
wall
formation
mechanisms
increased
division.
Our
results
show
has
outstanding
industrial
value
provides
comprehensive
understanding
it.