Growth balance analysis models of cyanobacteria for understanding resource allocation strategies DOI Creative Commons
Sajjad Ghaffarinasab, Martin J. Lercher, Hugo Dourado

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Апрель 18, 2023

Abstract Cyanobacteria have emerged as attractive microbial cell factories, they can convert atmospheric CO 2 and sunlight into valuable chemicals. To increase their growth productivity, one should aim to optimize the allocation of limited cellular resources across different metabolic processes. Here, we developed two balance analysis (GBA) models for cyanobacterium Synechocystis sp. PCC 6803. In its biological assumptions, closely related an existing coarse-grained model, while mathematical formulation is heavily streamlined. We show that GBA provide virtually identical predictions about resource among photosynthesis, carbon metabolism, protein translation machinery under environmental conditions previous, mathematically more involved model. Our model also captures effects photodamage on proteome resulting rates. further how be easily extended include reactions, leading a second capable new allocation. Balanced type presented here expanded details, providing useful toolbox understanding physiological capabilities cyanobacteria, resources, potential bioengineering optimized biomass production.

Язык: Английский

Synthetic, marine, light-driven, autotroph-heterotroph co-culture system for sustainable β-caryophyllene production DOI Creative Commons
W.Y. Chen, Young Kyoung Park, Lucie Studená

и другие.

Bioresource Technology, Год журнала: 2024, Номер 410, С. 131232 - 131232

Опубликована: Авг. 6, 2024

Applying low-cost substrate is critical for sustainable bioproduction. Co-culture of phototrophic and heterotrophic microorganisms can be a promising solution as they use CO

Язык: Английский

Процитировано

2
Nutraceutical prospects of genetically engineered cyanobacteria- technological updates and significance DOI

D. S. Tiwari,

Niwas Kumar,

Riya Bongirwar

и другие.

World Journal of Microbiology and Biotechnology, Год журнала: 2024, Номер 40(9)

Опубликована: Июль 9, 2024

Язык: Английский

Процитировано

2
Anti-echinococcal effect of metformin in advanced experimental cystic echinococcosis: reprogrammed intermediary carbon metabolism in the parasite DOI
Julia A. Loos,

Perla Negro,

Hugo H. Ortega

и другие.

Antimicrobial Agents and Chemotherapy, Год журнала: 2024, Номер unknown

Опубликована: Сен. 12, 2024

ABSTRACT Metformin, a safe biguanide derivative with antiproliferative properties, has shown antiparasitic efficacy against the Echinococcus larval stage. Hence, we assessed of dose 250 mg kg −1 day in experimental models advanced CE, at 6 and 12 months post-infection oral intraperitoneal administration, respectively. At this high dose, metformin reached intracystic concentrations between 0.7 1.7 mM triggered Eg-TOR inhibition through AMPK activation by AMP-independent -dependent mechanisms, which are dependent on drug dose. Cystic uptake was controlled increased expression organic cation transporters presence drug. In both models, reduced weight parasite cysts, altered ultrastructural integrity their germinal layers, availability glucose, limiting cellular carbon energy charge proliferative capacity metacestodes. This glucose depletion associated slight increase cystic 2-deoxiglucose transcriptional induction GLUT genes context, drastic glycogen consumption led to lactate production intermediary metabolism treated Specifically, fraction reducing soluble sugars decreased twofold, levels non-reducing sugars, such as sucrose trehalose, were modified fluid cells. Taken together, our findings highlight relevance promising candidate for CE treatment warrant further research improve therapeutic conditions chronic zoonosis humans.

Язык: Английский

Процитировано

2
Strong heterologous electron sink outcompetes alternative electron transport pathways in photosynthesis DOI Creative Commons
Michal Hubáček, Laura T. Wey, Robert Kourist

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 20, 2024

Abstract Improvement of photosynthesis requires a thorough understanding electron partitioning under both natural and strong sink conditions. We applied wide array state-of-the-art biophysical biochemical techniques to thoroughly investigate the fate photosynthetic electrons in engineered cyanobacterium Synechocystis sp. PCC 6803, blueprint for biotechnology, expressing heterologous gene ene-reductase, YqjM. This recombinant enzyme catalyses reduction an exogenously added substrate into desired product by utilising photosynthetically produced NAD(P)H, enabling whole-cell biotransformation. Through coupling biotransformation reaction with measurements, we demonstrated that artificial sink, outcompetes valves, flavodiiron protein-driven Mehler-like reaction, cyclic transport. These results show ferredoxin-NAD(P)H-oxidoreductase (FNR) is preferred route delivering from reduced ferredoxin cellular NADPH/NADP+ ratio as key factor orchestrating flux. insights are crucial molecular mechanisms transport harnessing sustainable bioproduction engineering source/sink balance. Furthermore, conclude identifying bioenergetic bottleneck prerequisite targeted platforms. Significance statement coupled biocatalytic (whole-cell biotransformation) performance model cyanobacteria. employed NAD(P)H enzyme, allowing us gain comprehensive partitioning. sinks

Язык: Английский

Процитировано

1
Salt and heat stress enhances hydrogen production in cyanobacteria DOI

Panayiotis-Ilias Broussos,

George E. Romanos,

Konstantinos Stamatakis

и другие.

Photosynthesis Research, Год журнала: 2024, Номер 161(1-2), С. 117 - 125

Опубликована: Март 28, 2024

Язык: Английский

Процитировано

1
Impact of irradiance and inorganic carbon availability on heterologous sucrose production in Synechococcus elongatus PCC 7942 DOI Creative Commons
Lisa Yun, Robert Zegarac, Daniel C. Ducat

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 8, 2024

Cyanobacteria have been proposed as a potential alternative carbohydrate feedstock and multiple species successfully engineered to secrete fermentable sugars. To date, the most productive cyanobacterial strains are those designed sucrose, yet there exist considerable differences in reported productivities across different model laboratories. In this study, we investigate how cultivation conditions (specifically, irradiance, CO 2 , cultivator type) affect productivity of sucrose-secreting Synechococcus elongatus PCC 7942. We find that S. produces highest sucrose yield irradiances far greater than what is often experimentally utilized, high light intensities tolerated by especially under higher density where turbidity may attenuate effective experienced culture. By increasing inorganic carbon availability, cscB / sps produced total 3.8 g L -1 within period being 47.8 mg h . This study provides quantitative description impact culture on cyanobacteria-derived assist standardize cross-laboratory comparisons demonstrates significant capacity improve via optimizing conditions.

Язык: Английский

Процитировано

1
Green microbes: Potential solutions for key sustainable development goals DOI Creative Commons
Sandra Díaz‐Troya, María José Huertas

Microbial Biotechnology, Год журнала: 2024, Номер 17(8)

Опубликована: Авг. 1, 2024

The latest assessment of progress towards the Sustainable Development Goals (SDGs) has identified major obstacles, such as climate change, global instability and pandemics, which threaten efforts to achieve SDGs even by 2050. Urgent action is needed, particularly reduce poverty, hunger change. In this context, microalgae are emerging a promising solution, in context food security environmental sustainability. As versatile organisms, offer nutritional benefits high-quality proteins essential fatty acids, can be cultivated non-arable areas, reducing competition for resources improving sustainability systems. role also includes other applications aquaculture, where they serve sustainable alternatives animal feed, agriculture, act biofertilizers biostimulants. These microorganisms play key interventions on degraded land, stabilizing soils, hydrological function increasing nutrient carbon availability. Microalgae therefore support several promoting agricultural practices contributing land restoration sequestration efforts. integration these areas mitigate impacts improve security, highlighting need increased research development, well public political support, exploit their full potential advance SDGs.

Язык: Английский

Процитировано

1
Synthetic Light‐Driven Consortia for Carbon‐Negative Biosynthesis DOI
Chao-Feng Li, Haotian Zheng,

Hengrun Li

и другие.

ChemBioChem, Год журнала: 2023, Номер 24(17)

Опубликована: Июль 4, 2023

Synthetic light-driven consortia composed of phototrophs and heterotrophs have attracted increasing attention owing to their potential be used in sustainable biotechnology. In recent years, synthetic phototrophic been produce bulk chemicals, biofuels, other valuable bioproducts. addition, autotrophic-heterotrophic symbiosis systems applications wastewater treatment, bioremediation, as a method for phytoplankton bloom control. Here, we discuss progress made on the biosynthesis microbial consortia. strategies optimizing are summarized. Moreover, highlight current challenges future research directions development robust controllable

Язык: Английский

Процитировано

2
Genome streamlining to improve performance of a fast-growing cyanobacteriumSynechococcus elongatusUTEX 2973 DOI Open Access
Annesha Sengupta, Anindita Bandyopadhyay, Debolina Sarkar

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 16, 2024

Cyanobacteria are photosynthetic organisms that have garnered significant recognition as potential hosts for sustainable bioproduction. However, their complex regulatory networks pose challenges to major metabolic engineering efforts, thereby limiting feasibility production hosts. Genome streamlining has been demonstrated be a successful approach improving productivity and fitness in heterotrophs but is yet explored its full phototrophs. Here we present the systematic reduction of genome cyanobacterium exhibiting fastest exponential growth, Synechococcus elongatus UTEX 2973. This work, first kind photoautotroph, involved an iterative process using state-of-the-art genome-editing technology guided by experimental analysis computational tools. CRISPR/Cas3 enabled large, progressive deletions predicted dispensable regions aided identification essential genes. The large were combined obtain strain with 55 kb reduction. strains streamlined showed improvement growth (up 23%) (by 22.7%) compared WT. strategy not only develop cyanobacterial improved traits can also facilitate better understanding phenome relationships.

Язык: Английский

Процитировано

0
Comprehensive understanding of the mutant ‘giant’ Arthrospira platensis developed via ultraviolet mutagenesis DOI Creative Commons
Changsu Lee, Sangil Han,

Ho Na

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 19, 2024

Introduction Cyanobacteria are typically of a size that can be observed under microscope. Here, we present cyanobacteria with the naked eye. Arthrospira platensis NCB002 strain showed differentiated morphological characteristics compared to previously reported spp. Methods was obtained by UV irradiation sp. NCB001, which isolated from freshwater and owned NCell Co., Ltd. A. NIES-39 National Institute for Environmental Studies (Tsukuba, Japan). We used various analytical techniques determine its overall characteristics. Results discussion The draft genome consists five contigs comprising 6,864,973 bp G+C content 44.3 mol%. had an average length 11.69 ± 1.35 mm maximum 15.15 mm, is 23.4–50.5 times longer than (0.3–0.5 mm) known spp., allowing it harvested using thin sieve. Transcriptome analysis revealed these differences resulted changes in cell wall formation mechanisms increased division. Our results show has outstanding industrial value provides comprehensive understanding it.

Язык: Английский

Процитировано

0