Communications in Soil Science and Plant Analysis,
Год журнала:
2024,
Номер
55(16), С. 2393 - 2407
Опубликована: Май 30, 2024
Soil
legacy
phosphorus
(P)
accumulated
due
to
the
long-term
continuous
application
of
P
fertilizers
in
agricultural
fields
soil
for
years,
as
sparingly
soluble
forms.
This
can
be
made
a
possible
source
using
P-activators.
In
this
study,
laboratory
incubation
and
pot
experiment
were
conducted
evaluate
potential
some
activators
(Phosphorus
solubilizing
bacteria,
phytase,
humic
acid,
oxalic
acid)
increasing
availability
residual
calcareous
soil.
For
experiment,
applied
alone
combination.
samples
analyzed
at
subsequent
intervals
available
P,
alkaline
phosphatase
activity,
sequential
fractions.
The
results
showed
that
phytase
with
acid
(HA)
increased
(12.4
kg
ha−1)
activity
(47.42
µg
p-nitrophenol
released
g−1
hr−1)
followed
by
Bacteria
(PSB)
PSB
treatments.
All
treatments
have
significant
effect
on
different
P-fractions.
A
greater
reduction
calcium
phosphate
fraction
was
noted
application.
combined
P-activators
performed
better
than
their
sole
best
three
selected
from
incubated
used
maize,
along
dosages
fertilizer.
morphological
parameters
observed
30th
60th
days
maize
crop.
plant
height,
chlorophyll
index,
dry
matter
production
HA
100%
recommended
dose
statistically
comparable
75%
P.
findings
illustrated
extent
release
availability.
25%
reduced
will
promote
soils
through
solubilization.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 28, 2025
The
potential
of
phosphate-solubilizing
microbes
(PSMs)
to
enhance
plant
phosphorus
uptake
and
reduce
fertilizer
dependency
remains
underutilized.
This
is
partially
attributable
frequent
biofertilizer-farming
system
misalignments
that
efficacy,
an
incomplete
understanding
underlying
mechanisms.
study
explored
the
seed
microbiomes
nine
Australian
lucerne
cultivars
identify
characterize
high-efficiency
PSMs.
From
a
library
223
isolates,
94
(42%)
exhibited
phosphate
solubilization
activity
on
Pikovskaya
agar,
with
15
showing
high
efficiency
(PSI
>
1.5).
Genomic
analysis
revealed
"high-efficiency"
belonged
four
genera
(Curtobacterium,
Pseudomonas,
Paenibacillus,
Pantoea),
including
novel
strains
species.
However,
key
canonical
genes,
such
as
pqq
operon
gcd,
did
not
reliably
predict
phenotype,
highlighting
limitations
in
silico
predictions.
Mutagenesis
isolate
Pantoea
rara
Lu_Sq_004
generated
mutants
enhanced
null
phenotypes,
revealing
role
"auxiliary"
genes
downstream
function
pathways.
Inoculation
studies
seedlings
demonstrated
significant
increase
shoot
length
(p
<
0.05)
following
treatment
enhanced-solubilization
mutant,
indicating
promising
growth-promotion
effect.
These
findings
highlight
more
personalized
"system-appropriate"
biofertilizers
underscore
importance
integrating
genomic,
phenotypic,
planta
analyses
validate
function.
Further
research
required
investigate
links
between
genomic
markers
functional
outcomes
optimize
development
sustainable
agricultural
inputs.
Phytic
acid
and
its
salts
are
the
major
storage
form
of
both
phosphate
myo-inositol,
especially
in
cereals.
chelates
multivalent
cations,
which
decreases
solubilized
these
nutrients
their
dietary
bioavailability
for
absorption
assimilation.
Increasing
micronutrient
from
economic
phytate-rich
food
can
be
achieved
by
subjecting
this
to
fermentation
phytase-producing
bacteria.
A
total
8
lactic
bacteria
were
isolated
different
fermented
samples.
Among
tested
ability
produce
phytase,
only
five
isolates
selected
as
promising
producers.
The
results
revealed
that
isolate
No.
4
produced
highest
phytase
levels
411
U/mL.
differentially
able
tolerate
acidic,
alkaline,
heat,
surfactant,
osmotic,
bile,
pancreatic
enzyme
stresses,
with
superiority
4,
demonstrated
most
desirable
probiotic
potentials
confirmed
principal
component
analysis
(PCA),
heat
map,
network
analysis.
Isolate
was
identified
16S
rRNA
sequencing
later
Pediococcus
pentosaceus
strain
NMP4762Ch
under
accession
number
MZ413646.
Moreover,
active
cells
P.
immobilized
alginate;
reusability
resulted
continuous
production
an
optimum
activity
432.0
U/mL
after
14
days,
decreased
time
reach
142.5
56
days.
maximum
shelf
stability
also
observed,
557.5
U/mL,
days
declined
time,
reaching
133.5
end
56th
day.
Since
humans
don't
naturally
utilizing
probiotics
is
important
combat
mineral
deficiencies.
