Synergistic Effect of Different Soilless Substrates on the Hardening of In- vitro Raised Banana (Musa sp.) Saplings of Grand Naine DOI Open Access

Sangam,

Pragati Misra, Shashi Shekhar

и другие.

International Journal of Plant & Soil Science, Год журнала: 2023, Номер 35(16), С. 147 - 156

Опубликована: Июнь 22, 2023

The present study was carried out to investigate the impact of different substrates on primary and secondary hardening tissue culture raised banana plantlets. Among eight treatment combinations potting mixtures, PHT7 which contained cocopeat with combination perlite vermiculite (2:1:1) emerged as best media survival percentage 98.90% comparative better growth during hardening. Simultaneously maximum development including plant height (15.36 cm), number leaves (5.71), pseudostem girth (3.88 roots (5.90), root length (3.69 cm) has been observed For hardening, were used in conjunction soil. all treatments SHT4 (soil+ bonemeal; 3:1) along application Jeevamrit have shown highest field survivability (100%). Moreover a shoot (33.40 recorded (9.25) pseudo stem 6.98 cm. outcome suggested that performed However comparatively higher

Язык: Английский

Fungal Endophytes of Moringa (Moringa oleifera L.), Neem (Azadirachta indica) and Lavender (Lavandula angustifolia) and Their Biological Control of Fusarium Wilt of Banana DOI Creative Commons

Bonface M. Nthuku,

Esther Wanja Kahariri,

Johnson K. Kinyua

и другие.

Microbiology Research, Год журнала: 2023, Номер 14(4), С. 2113 - 2132

Опубликована: Дек. 15, 2023

Fusarium wilt, caused by oxysporum f. sp. cubense (Foc), significantly affects the productivity of banana crop in field. Currently, there are no effective control measures available, and therefore, is an urgent need to develop novel strategies spread disease. Biological a promising strategy for management The aim this study was identify fungal endophytes associated with Moringa (Moringa oleifera), Neem tree (Azadirachta indica) Lavender (Lavandula angustifolia) their antifungal activities against tropical race 4 (Foc TR4). We isolated 69 from different tissues M. oleifera, A. indica L. angustifolia screened activity Foc TR4. Six exhibited highest antagonistic TR4 based on dual-culture assays. Based morphology ITS gene sequence analysis, selected six were identified be related Alternaria alternata (MB7 NR3), Neofusicoccum parvum (LB1), (LR1), Talaromyces amestolkiae (MB14) tenuissima (NB6). culture filtrates (LB1, LR1, MB7, MB14, NB6 NR3) more than 50% inhibition mycelial growth vitro producers β-1,3-glucanase. showed biocontrol efficacy wilt pot experiments. findings demonstrate that LB1, NR3 should explored as agents biofertilizers production.

Язык: Английский

Процитировано

6

COMBINATION OF BAP AND TDZ IN INDUCING THE GROWTH OF BANANA SHOOTING EXPLANT CV. BARANGAN IN VITRO DOI Creative Commons

Eliyanti,

Zulkarnain Zulkarnain, Budiyati Ichwan

и другие.

Russian Journal of Agricultural and Socio-Economic Sciences, Год журнала: 2024, Номер 145(1), С. 86 - 94

Опубликована: Янв. 25, 2024

Barangan banana (Musa acuminata L.) is one of the superior types in West Tanjung Jabung district, Jambi Province -Indonesia.Barangan bananas are very popular with public and have high economic value.The problem faced conventional cultivation development that it difficult to obtain quality seeds large uniform quantities, so overcome this we need alternative technology for propagating through modern techniques such as plant tissue culture techniques.The advantage technique able produce en masse uniformly a relatively short time.In addition, resulting progeny also conditioned be free from pests diseases, especially diseases caused by bacteria fungi.

Язык: Английский

Процитировано

0

Biohardening with endomicrobiome – A novel approach to develop Fusarium wilt resistance in banana (Musa spp.) DOI Creative Commons

Dipti Pandurang Mohite,

M. Kavino,

S. Nakkeeran

и другие.

The Microbe, Год журнала: 2024, Номер 4, С. 100109 - 100109

Опубликована: Июль 3, 2024

Fusarium wilt of banana (Musa spp.) primarily caused by a soil-borne fungus oxysporum f. sp. cubense (Foc), presents significant threat to the industry worldwide. To propagate bananas efficiently, tissue culture techniques are commonly employed, yield quality planting material with desirable traits and devoid harmful microorganisms. However, cultured plantlets may be more susceptible than traditional due lack indigenous beneficial endophytes. Endophytes microorganisms that colonize internal tissues without causing pathogenicity have been found occur in all plant species. They form symbiotic relationships host plants, enhancing their performance. also promote growth production secondary metabolites like auxin gibberellins. Additionally, some compounds act as antibiotic antifungal thus inhibiting pathogen invasion its growth. Since applied endomicrobiome occupies niche areas Foc corm, these microbes not only avoid entry but plant's development metabolites. This is promising biological solution for sustainable cultivation era "climate change chemical free farming".

Язык: Английский

Процитировано

0

Integrated control of Fusarium wilt in banana by Bacillus velezensis EB1 and potassium sorbate DOI Creative Commons
Siwen Liu, Wenlong Yang, Xiaofang Yang

и другие.

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Ноя. 6, 2024

Fusarium wilt of banana, caused by oxysporum f. sp. cubense tropical race 4 (Foc TR4), is a widely distributed soilborne disease that poses serious threat to banana production. Many control measures have been implemented but not effective. Here, we evaluated combined strategy for involves biological agent (Bacillus velezensis strain EB1) and bioactive compound (potassium sorbate). Our results showed potassium sorbate inhibited Foc TR4 in dose-dependent manner. Potassium did limit the growth EB1 vitro; instead, it promoted antagonistic ability upregulating expression antagonism-related genes. In greenhouse experiments, application significantly reduced index suppressing fungal roots promoting plant growth. Overall, our demonstrated B. can be used together sustainable management wilt.

Язык: Английский

Процитировано

0

Development Multiplex–PCR Technique for Concurrent Detection of Taxonomically Different DNA Viruses in Banana (Musa acuminata) Cv. Canvendish DOI Creative Commons
Rushikesh Tahakik

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 12, 2024

Abstract The Banana bunchy top virus (BBTV) and Cucumber mosaic (CMV) pose significant threats to banana production, highlighting the need plant virus-free specimens maximize yield control disease spread. Early detection is crucial, as BBTV symptoms typically appear 25 85 days after being transmitted by aphids (Pentalonia negronervosa). In this study, we designed multiplex PCR primers targeting key viral regions: movement protein-encoding genes (MV), replication-associated re- gion (RP), coat protein (CP) for both CMV. Samples were collected from various agroclimatic zones within Maharashtra’s banana-growing regions. An internal primer was included identify any reaction failures. Our results show that a highly effective method screening in bananas, reducing occurrence of false positives often seen single-gene amplification. Based on our findings, recommend use markers routine testing plants improve management productivity cavendish cultivation.

Язык: Английский

Процитировано

0

Molecular and Growth Responses of Musa acuminata var. Barangan Post Application of Beneficial Endophytic Bacteria DOI Creative Commons

Hanifah Yusadi,

Arief Pambudi, Yunus Effendi

и другие.

Jurnal Ilmu Pertanian Indonesia, Год журнала: 2024, Номер 30(1), С. 40 - 47

Опубликована: Дек. 27, 2024

Endophytic bacteria reside in plants' roots and can benefit plant growth. The study aimed to evaluate the application of an endophytic bacterial consortium enhancing growth banana plants identify genes that maximally assist nutrient utilization during One-month-old were inoculated by soaking them for one hour a 500 mL suspension bacteria. In vivo observations conducted greenhouse at Al-Azhar University Indonesia over 40 days, which morphological physiological calculated. results revealed control exhibited lower than those treated with led increased barangan plants, as evidenced improvements leaf length, width, height, chlorophyll levels. Furthermore, semi-quantitative analysis Cytobacillus depressus, Bacillus stratophericus, B. mycoides significantly contributing These genes, including WRKY33, Ma03_92660, Ma01_901890, Ma04_936790, Pho-1,2, their highest expression levels starting from 28th day experiment. Keywords: growth, bacteria, real-time PCR,

Язык: Английский

Процитировано

0

Integrated biological and chemical control of Fusarium wilt by Bacillus velezensis EB1 and potassium sorbate DOI
Siwen Liu, Wenlong Yang, Xiaofang Yang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 28, 2024

Abstract Banana wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is a globally distributed soilborne disease that poses serious threat to banana production. Many different control measures have been implemented but not proven effective enough. Here, combined strategy of Fusarium involving the recognized biological agent B. velezensis EB1 and bioactive compound potassium sorbate was evaluated. Our results showed inhibited Foc TR4 in dose-dependent manner. Potassium did affect growth vitro; instead, it promoted its antagonistic ability upregulating expression genes. In greenhouse experiments, application significantly reduced index suppressing fungal roots while promoting plant growth. Overall, our indicate with has strong potential for sustainable management wilt.

Язык: Английский

Процитировано

0

Bacillus Velezensis Yn2111 Promotes Plant Growth, But Exacerbates Fusarium Wilt of Banana by Blocking the Expression of Cavendish Banana Amine Oxidase Muao DOI

Hongwei Yu,

Guangdong Zhou, Wenlong Zhang

и другие.

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0

Diversity of Endophytic Fungi in Banana Cultivars of Assam India DOI Open Access

Jibanjyoti Panda,

P. Vetrivelkalai,

B. Bhagawati

и другие.

Journal of Botanical Research, Год журнала: 2023, Номер 5(2), С. 49 - 58

Опубликована: Апрель 21, 2023

Endophytic fungal isolates (139 no.) were obtained from 143 (62 roots, 18 fruits and 54 leaves) samples of 15 different varieties banana collected 10 sites in Assam, India during 2018-2019. Overall isolation frequency surface-sterilized tissue ranged 10%-80% (as per site) 6%-70% variety banana). All segregated into 40 types on the basis macromorphological micro morphological characteristics. Forty taxa isolated belonging to 14 genera including Absidia, Arthrinium, Aspergillus, Bipolaris, Cladosporium, Curvularia, Dendrophion, Fusarium, Humicola, Mortierella, Mucor, Penicillium, Paecilomyces, Verticillium one mycelium sterile. Among them, Cladosporium cladosporioidies Paecilomyces sp. frequently occurred most surveyed whereas Cladospoirum cladosporioides Aspergillus 8, Fusarium graminseram varieties. However, all differed their diversity. Banana Narigoan Jorhat have been found with maximum species followed by marigoan so as Amrit Sagar endowed 27 no. fungi Jehaji Honda which associated a Isolation relative abundance cladosporiodes (80%, 4.6), farinosus 4.6) Penicillium ruburm, 8 & 9 (70%, 4.02) recorded comparatively sites. Mortieralla Pacilomyces are (73.33%, 4.93).

Язык: Английский

Процитировано

1

Synergistic Effect of Different Soilless Substrates on the Hardening of In- vitro Raised Banana (Musa sp.) Saplings of Grand Naine DOI Open Access

Sangam,

Pragati Misra, Shashi Shekhar

и другие.

International Journal of Plant & Soil Science, Год журнала: 2023, Номер 35(16), С. 147 - 156

Опубликована: Июнь 22, 2023

The present study was carried out to investigate the impact of different substrates on primary and secondary hardening tissue culture raised banana plantlets. Among eight treatment combinations potting mixtures, PHT7 which contained cocopeat with combination perlite vermiculite (2:1:1) emerged as best media survival percentage 98.90% comparative better growth during hardening. Simultaneously maximum development including plant height (15.36 cm), number leaves (5.71), pseudostem girth (3.88 roots (5.90), root length (3.69 cm) has been observed For hardening, were used in conjunction soil. all treatments SHT4 (soil+ bonemeal; 3:1) along application Jeevamrit have shown highest field survivability (100%). Moreover a shoot (33.40 recorded (9.25) pseudo stem 6.98 cm. outcome suggested that performed However comparatively higher

Язык: Английский

Процитировано

0