The role of gut microbiota at different developmental stages in the adaptation of the Etiella zinckenella to a plant host

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 10, 2025

Insect gut symbiotic microbiota play a crucial role in the nutritional, physiological, and behavioral aspects of their hosts, providing valuable insights for investigating co-evolution insects plants. Sophora alopecuroides L. serves as an important windbreak plant, while Etiella zinckenella is major pest that infests its seeds. However, structure community E. remains poorly understood. In this study, we analyzed across different developmental stages—larvae (1st—5th instars), pupae, adults—infesting S. using 16 S rRNA high-throughput sequencing. The results revealed dominant phyla throughout development were Proteobacteria Bacteroidota, although genera varied significantly stages. Diversity analysis at stages indicated microbial diversity was higher larval stage compared to pupal adult Functional predictions further highlighted richness metabolic pathways within zinckenella. Notably, carbohydrate metabolism functions more abundant during stage, lipid substantially lower. Our findings demonstrate dynamic changes composition zinckenella, underscoring critical roles these bacteria specific insect's life cycle. This study lays groundwork future strategies aimed controlling through modulation microbiota, offering significant theoretical implications.

Язык: Английский

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Gut bacteria symbiosis affects cuticular hydrocarbon profile and mating success in wild Ceratitis capitata (Diptera: Tephritidae) males

Austral Entomology, Год журнала: 2025, Номер 64(1)

Опубликована: Фев. 1, 2025

Abstract Ceratitis capitata (Diptera: Tephritidae) is a highly polyphagous species and seriously pest with significant economic importance, having great number of studies focused in its management. Recently, there has been surge interest the symbiotic relationship between gut bacteria their insect hosts. In this context, objective work was to evaluate role microbiota on aposymbiotic wild medfly adult sexual behaviour, especially mate choice mating success. We also latency time, sperm transference, testes ovarian sizes female fecundity. Finally, we compared cuticular hydrocarbon profiles explore other possible mechanisms that mediate For purpose, teneral adults were separated into two groups, aposymbiotic. Aposymbiotic treated an antibiotic mixture (tetracycline plus ciprofloxacin) until maturity. Results behaviour assays showed males more successful comparison males, preference could be related greater organ development females differences male profiles. support hypothesis presence can play beneficial behaviour. Knowledge about importance hydrocarbons acting recognition obtained. This first report influence component exoskeleton for might useful improvement sustainable strategies management such as sterile technique.

Язык: Английский

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Investigating gut bacteria in field populations of pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), in India

Biologia, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Язык: Английский

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Intestinal Microbiota and Vaccinations: A Systematic Review of the Literature

Vaccines, Год журнала: 2025, Номер 13(3), С. 306 - 306

Опубликована: Март 12, 2025

Background: Vaccination constitutes a low-cost, safe, and efficient public health measure that can help prevent the spread of infectious diseases benefit community. The fact vaccination effectiveness varies among populations, causes this are still unclear, indicates several factors involved should be thoroughly examined. “intestinal microbiota” is most crucial these elements. Numerous clinical studies demonstrate intestinal microbiota’s significance in determining alleged “immunogenicity” efficacy vaccines. This systematic review aimed to all relevant scientific literature highlight role microbiota COVID-19, Salmonella typhi, Vibrio cholerae, rotavirus vaccinations. Materials Methods: MESH terms “vaccines” “microbiota” were used search major databases PubMed, SciVerse Scopus, Web Knowledge, Cochrane Central Register Controlled Clinical Trials. Results: Between February 2024 October 2024, analysis was conducted using electronic databases, yielding total 235 references. Finally, 24 RCTs chosen after meeting inclusion criteria: eight two three eleven rotavirus. Only six demonstrated good study quality with Jadad score or four. Conclusions: According review’s results, surely plays vaccinations’ enhanced immunogenicity, especially younger people. As it unclear what mechanisms underlie effect, more research needed better understand microbiota.

Язык: Английский

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Inside the Belly of the Beast: Exploring the Gut Bacterial Diversity of Gonipterus sp. n. 2

Microbial Ecology, Год журнала: 2025, Номер 88(1)

Опубликована: Апрель 12, 2025

The Eucalyptus snout beetle (Gonipterus sp. n. 2) is a destructive invasive pest of plantations, responsible for significant defoliation and wood yield losses globally. Native to Australia, this has adapted thrive on diverse hosts, overcoming their chemical defences. However, the mechanisms by which Gonipterus tolerates or utilises these plant defence metabolites remain poorly understood. In South Africa, 2 poses threat plantations causing extensive leading substantial reductions in growth production. This study investigates relationship between diet, host species, gut microbiome 2. Using controlled feeding experiments, beetles were reared artificial, semi-artificial, natural diets, as well two genotypes with distinct secondary metabolite profiles. High-throughput 16S rDNA sequencing gas chromatography-mass spectrometry (GC-MS) revealed shifts bacterial diversity composition across diets. Natural diets supported most microbial communities, while artificial fostered homogenised dominated opportunistic taxa like Serratia. Host-specific effects observed frass microbiota, biotransformation monoterpenes into less toxic derivatives. results highlight plasticity enables metabolic adaptability resilience environments. flexibility underpins invasiveness Gonipterus, emphasising role symbionts Understanding interactions offers novel insights microbiome-targeted management strategies, providing sustainable approach mitigate impact global forestry.

Язык: Английский

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Trypanosoma cruzi/Triatomine Interactions—A Review

Pathogens, Год журнала: 2025, Номер 14(4), С. 392 - 392

Опубликована: Апрель 17, 2025

This review summarizes the interactions between Trypanosoma cruzi, etiologic agent of Chagas disease, and its vectors, triatomines, highlights open questions. Four important facts should be emphasized at outset: (1) The development T. cruzi strains their with mammalian host insect vector vary greatly. (2) Only about 10 over 150 triatomine species have been studied for protozoan parasite. (3) use laboratory triatomines makes generalizations difficult, as maintenance conditions influence interactions. (4) intestinal microbiota is involved in interactions, but mutualistic symbionts, Actinomycetales, so far only identified four triatomines. effects on are reflected a different colonization ability species. In addition, intestine lead to strong multiplication posterior midgut rectum, infectious metacyclic trypomastigotes developing almost exclusively latter. Starvation feeding induce certain stages cruzi. negative depend strain particularly evident when stressed. immunity responds ingested blood-stage some affects many bacteria, not all symbionts. specific interaction bacteria after knockdown antimicrobial peptides: number non-symbiotic increases decreases. long-term infections, suppression indicated by growth microbiota.

Язык: Английский

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Prospects for deploying microbes against tree-killing beetles (Coleoptera) in Anthropocene

Frontiers in Forests and Global Change, Год журнала: 2023, Номер 6

Опубликована: Июль 26, 2023

Forests form rich biodiversity hubs that act as large reservoirs of natural carbon. The spatial and temporal heterogeneity these complex habitats forest floors provides ecological services immense socio-economic importance. However, hotspots are incessantly exposed to multifarious abiotic, biotic, anthropogenic disturbances, amongst which unpredictable pest (i.e., bark beetle) outbreak account for the loss vegetation microbiome measurable quantum. importance in forming an inseparable functional unit every host shaping its interaction with other partners has been well realized. Interestingly, pests, including beetles, also reported rely on their endosymbiotic microbial manipulate tree defense machinery. In contrast, holobiont trees regulates overall function fitness significantly contributes tackling challenging situations. Nevertheless, how directly or indirectly influence beetle is still enigma. present review shall elaborate role tools enhancing performance fitness, helps counter damage. Besides, it emphasize exploiting microorganisms acting biocontrol agents shielding against destruction. application endosymbiont-mediated RNA interference (RNAi) working two-tier specificity controlling devastations be discussed new-age technological advances. All explanations expected put forth potential toolbox offering better more sustainable management strategies future.

Язык: Английский

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Construction of a beneficial microbes‐enriched rhizosphere system assists plants in phytophagous insect defense: current status, challenges and opportunities

Pest Management Science, Год журнала: 2024, Номер 80(11), С. 5608 - 5618

Опубликована: Июль 10, 2024

Abstract The construction of a plant rhizosphere system enriched with beneficial microbes (BMs) can efficiently help plants defend against phytophagous insects. However, our comprehensive understanding this approach is still incomplete. In review, we methodically analyzed the progress made over last decade, identifying both challenges and opportunities. main methods for developing BMs‐enriched include inoculating exogenous BMs into plants, amending existing soil microbiomes amendments, utilizing to shape microbiomes. assist in suppressing insects across many orders, including 13 Lepidoptera, seven Homoptera, five Hemiptera, Coleoptera, four Diptera, one Thysanoptera species by inducing systemic resistance, enhancing tolerance, augmenting secondary metabolite production, directly herbivores. Context‐dependent factors such as abiotic biotic conditions, well response insect herbivores, affect outcomes BM‐assisted defense. Several opportunities have emerged, development synthetic microbial communities herbivore control, integration biosensors effectiveness assessment, confirmation BM targets defense, regulation via smart farming artificial intelligence. This study offers valuable insights BM‐enriched within an integrated pest management approach. © 2024 Society Chemical Industry.

Язык: Английский

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Microbial Symbiont-Based Detoxification of Different Phytotoxins and Synthetic Toxic Chemicals in Insect Pests and Pollinators

Journal of Xenobiotics, Год журнала: 2024, Номер 14(2), С. 753 - 771

Опубликована: Июнь 4, 2024

Insects are the most diverse form of life, and as such, they interact closely with humans, impacting our health, economy, agriculture. Beneficial insect species contribute to pollination, biological control pests, decomposition, nutrient cycling. Pest can cause damage agricultural crops vector diseases humans livestock. often exposed toxic xenobiotics in environment, both naturally occurring toxins like plant secondary metabolites synthetic chemicals herbicides, fungicides, insecticides. Because this, insects have evolved several mechanisms resistance xenobiotics, including sequestration, behavioral avoidance, enzymatic degradation, many cases had developed symbiotic relationships microbes that aid this detoxification. As research progresses, important roles these health function become more apparent. Bacterial symbionts degrade phytotoxins allow host feed on otherwise chemically defended plants. They also confer pesticide their hosts, especially frequently treated fields. It is study interactions between order further understanding pest mitigate negative effect pesticides nontarget Hymenopteran pollinators.

Язык: Английский

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The biochemical and molecular mechanisms of plants: a review on insect herbivory

Plant Signaling & Behavior, Год журнала: 2024, Номер 20(1)

Опубликована: Дек. 26, 2024

Biochemical and molecular mechanisms have been essential to reduce various insect attacks on plants. The biochemical methods are wide involving direct indirect defenses. defensive chemical substances secreted effectively the wound caused by herbivores (insects phytopathogens) Plants responded producing VOCs which draw natural enemies of insects phytopathogens. progress observed in cognition stimulus plants their potential control responses is characterized modification shifts our attention development endogenous resistance preserving crops. main objective implementing a biotechnological mechanism crop production employ durable multimechanistic alternatives pests via plant produces upon encountering attack.

Язык: Английский

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