Characterization of Lactobacillus spp. as Probiotic and Antidiabetic Potential Isolated from Boza, Traditional Fermented Beverage in Turkey

International Journal of Microbiology, Год журнала: 2024, Номер 2024(1)

Опубликована: Янв. 1, 2024

Boza, a cereal‐based beverage popular in southeast Europe, is fortified with probiotics and believed to positively impact the composition of gut microflora. This investigation focused on fermented boza identify strains probiotic Lactobacillus spp. capable inhibiting carbohydrate‐hydrolysing enzymes α ‐glucosidase (AG) ‐amylase (AA). The isolated bacterial underwent comprehensive assessment, including biochemical, molecular, trait analyses such as tolerance survivability, adhesion, safety, health‐promoting attributes. We evaluated inhibitory potential supernatant, cell lysate, intact cells Molecular analysis has revealed that isolates RAMULAB30 RAMULAB29 exhibit significant genetic similarity (>97%) Lacticaseibacillus paracasei Limosilactobacillus fermentum , respectively. These findings are documented NCBI database. They exhibited resistance gastrointestinal intestinal fluids, also indicating their for adhesion. Additionally, showed antibacterial activity, particularly against Micrococcus luteus . vancomycin methicillin antibiotics but were more susceptible streptomycin ampicillin. Furthermore, demonstrated antioxidant properties. To ensure haemolytic assay was conducted despite general recognition safe (GRAS) status. study primarily aimed evaluate effects extract AG AA. Bacterial activity both enzyme (32%–67% inhibition) AA (18%–46% different forms, supernatant (CS), lysed (CE), (IC). underscore inhibit effectively. L. remarkable antidiabetic potential. Food products incorporating these have promising prospects nutraceuticals, providing improved health benefits.

Язык: Английский

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Exploring the probiotic potential of Lactiplantibacillus pentosus SM1: resistance, anti-microbial activity, anti-biofilm, cytotoxic activity, and safety properties

LWT, Год журнала: 2024, Номер unknown, С. 116850 - 116850

Опубликована: Сен. 1, 2024

Язык: Английский

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EXPLORING JACKFRUIT FLOUR POLYPHENOLS AS PROMISING SGLT-2 INHIBITORS FOR HYPERGLYCEMIA MANAGEMENT

International Journal of Applied Pharmaceutics, Год журнала: 2025, Номер unknown, С. 199 - 208

Опубликована: Янв. 7, 2025

Objective: This study explored the potential of dietary polyphenols from whole green jackfruit flour as natural Sodium-Glucose Co-Transporter-2 (SGLT-2) inhibitors for managing hyperglycemia in diabetes mellitus. Methods: Advanced bio-computational techniques, including molecular docking, Molecular Dynamics (MD) simulations, and binding free energy calculations, were employed to identify assess flour. Caffeic syringic acids highlighted their strong affinities SGLT-2 receptor. Additionally, a ligand-based pharmacophore model was developed using caffeic acid reference screen new lead compounds commercial product databases. Results: The found that exhibited stronger more stable interaction profiles with receptor than standard drug empagliflozin. MD simulations demonstrated these provided greater stability site, indicating efficacy inhibitors. screening further supported findings, identifying both promising candidates. Among 14 obtained High-Performance Liquid Chromatography (HPLC), docking suggested (binding affinity:-9.0 kcal/mol) affinity:-9.1 compared empagliflozin affinity:-10.4 kcal/mol). Further, dynamics through Root mean Square Deviation (RMSD), Fluctuation (RMSF), Radius Gyration (Rg), Solvent Accessible Surface Area (SASA), ligand hydrogen bonds. Conclusion: is first effective These show significant novel agents findings support exploration plant-derived therapies treatment.

Язык: Английский

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Examining the Impact of Probiotic Lactiplantibacillus pentosus 6MMI on Inhibiting Biofilm Formation, Adhesion, and Virulence Gene Expression in Listeria monocytogenes ATCC 19115

Biofilm, Год журнала: 2025, Номер 9, С. 100255 - 100255

Опубликована: Янв. 18, 2025

Язык: Английский

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Isolation, Characterization and Probiotic Properties of Levilactobacillus brevis FS1 from Fermented Food and Its Impact on Differentiation and Fat Deposition

Indian Journal of Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Язык: Английский

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Fermented sugarcane juice-derived probiotic Levilactobacillus brevis RAMULAB54 enhances lipid metabolism and glucose homeostasis through PPAR-γ activation

Frontiers in Microbiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 29, 2025

The gut microbiota plays a significant role in metabolic disorders such as diabetes and obesity, with the peroxisome proliferator-activated receptor gamma (PPAR-γ) being key regulator adipogenesis glucose metabolism. This study is novel approach that explores antihyperglycemic antihyperlipidemic effects of Levilactobacillus brevis RAMULAB54 (LB13243), isolated from fermented sugarcane juice. LB13243 was cultured for SEM imaging, its supernatant (LBR54) analyzed. Organic acid interactions PPAR-γ were evaluated via molecular docking, while cytotoxicity adipocyte differentiation 3T3-L1 cells tested using MTT assays, Oil Red O staining, triglyceride quantification, qRT-PCR. In vivo , male Wistar rats hyperlipidemic streptozotocin-induced hyperglycemic models treated 4 weeks, followed by analysis food intake, body weight, serum glucose, lipids, histopathology. inhibited carbohydrate-hydrolyzing enzymes showed an organic profile. silico hydroxycitric had similar binding to PPARγ rosiglitazone (binding energy:−8.4 kcal/mol vs.−8.3 kcal/mol), greater stability (RMSD: 1.2 Å vs. 1.7 Å). Pharmacokinetics indicated moderate GI absorption (20%) low toxicity acid. LBR54 did not affect cell viability but reduced lipid accumulation 13% triglycerides ≤ 44 mg/dL. qRT-PCR revealed upregulation C/EBP-α, downregulation FAS, suggesting modulation adipogenesis. weight gain, normalized organ weights rats, improving levels profiles models. Histopathology improved tissue structure, indicating LB13243's potential reduce hyperglycemia hyperlipidemia modulating metabolism inflammation. suggests it promising natural option managing hyperlipidemia. also highlights distinctive capability modulate through acids, particularly acid, providing insights into therapeutic potential.

Язык: Английский

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A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types

STAR Protocols, Год журнала: 2025, Номер 6(1), С. 103619 - 103619

Опубликована: Фев. 5, 2025

Язык: Английский

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Isolation, Probiotic Characterization and Whole-Genome Sequencing of Gut Faecalibacterium prausnitzii

Human Nutrition & Metabolism, Год журнала: 2025, Номер unknown, С. 200315 - 200315

Опубликована: Апрель 1, 2025

Язык: Английский

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Investigating the antidiabetic efficacy of dairy-derived Lacticaseibacillus paracasei probiotic strains: modulating α-amylase and α-glucosidase enzyme functions

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Дек. 4, 2023

The current study aims to evaluate and characterize the probiotic andantidiabetic properties of lactic acid bacteria (LAB) obtained from milk other dairy-based products. strains were tested physiologically, biochemically, molecularly. Based on biochemical tests 16S rRNA gene amplification sequencing, all three isolates RAMULAB18, RAMULAB19, RAMULAB53 identified as Lacticaseibacillus paracasei with homology similarity more than 98%. inhibitory potential each isolate against carbohydrate hydrolysis enzymes (α-amylase α-glucosidase) was assessed using different preparations RAMULAB (RL) isolates: supernatant (RL-CS), intact cells (RL-IC), cell-free extraction (RL-CE). Additionally, evaluated for its antioxidant activity free radicals (DPPH ABTS). strain's RL-CS, RL-CE, RL-IC inhibited α-amylase (17.25 55.42%), α-glucosidase (15.08-59.55%), DPPH (56.42-87.45%), ABTS (46.35-78.45%) differently. With highest survival rate (>98%) toward tolerance gastrointestinal conditions, hydrophobicity (>42.18%), aggregation (>74.21%), well attachment an individual's colorectal cancer cell line (HT-29) (>64.98%), human buccal chicken crop epithelial cells, exhibited extensive results. All high resistance antibiotics (methicillin, kanamycin, cefixime, vancomycin), assays such antibacterial, DNase, hemolytic, gelatinase performed safety assessment. Results suggest that LAB described are valuable candidates their significant health benefits they can also be utilized a beginning or bio-preservative tradition in food, agriculture, pharmaceutical sectors. excellent vitro applicants yet additional vivo testing is required.

Язык: Английский

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Sauerkraut‐derived LAB strains as potential probiotic candidates for modulating carbohydrate digestion attributing bacterial organic acid profiling to antidiabetic activity

Food Science & Nutrition, Год журнала: 2024, Номер 12(11), С. 9682 - 9701

Опубликована: Окт. 23, 2024

Abstract Sauerkraut‐derived lactic acid bacterial (LAB) strains have gained attention due to their potential health benefits. This study focuses on evaluating seven RAMULAB isolated from sauerkraut, aiming identify promising candidates for modulating α‐glucosidase (AG) and α‐amylase (AM) enzymatic functions. with remarkable probiotic can contribute the digestive manage conditions like diabetes. Identifying robust a fermented food, holds promise natural cost‐effective sources. The underwent extensive characterization, including identification through 16S ribosomal RNA (rRNA) sequencing. Their tolerance harsh conditions, adherence properties, antimicrobial activity, antioxidant potential, inhibition of AG AM were assessed. In silico analyses explored molecular interactions, particularly hydroxycitric acid, antidiabetic compound. Among strains, RAMULAB48 emerged as standout candidate. It displayed exceptional resilience acidic bile (≥97%), simulated gastrointestinal (≥95%), highlighting its suitability applications. exhibited cell‐surface hydrophobicity (80%), autoaggregation (42%), coaggregation pathogens (≥33%), adhesion epithelial cells. Additionally, all isolates demonstrated gamma‐hemolysis resistance antibiotics (Kanamycin, Methicillin, Vancomycin), while displaying strong antibacterial properties against foodborne pathogens. These also varying degrees highest (≥41%). terms cell‐free supernatant (CS) obtained expressed levels, notably inhibiting yeast by an impressive 59.55% being 67.42%. produced organic acids, (28.024 mg/mL), which showed in analyses, indicating favorable interactions target enzymes. identifies Lacticaseibacillus paracasei RAMULAB48, strain, candidate tolerance, effects. presence further underscores managing

Язык: Английский

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