Bifidobacterium longum S3 alleviates loperamide-induced constipation by modulating intestinal acetic acid and stearic acid levels in mice

Food & Function, Год журнала: 2024, Номер 15(11), С. 6118 - 6133

Опубликована: Янв. 1, 2024

Bifidobacterium longum S3 influenced the intestinal barrier and immunity, water channel protein expression, neurotransmitter GI active peptide levels by affecting immunity modulating changes in of fatty acid metabolites, especially LCFAs.

Язык: Английский

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The Different Ways Multi-Strain Probiotics with Different Ratios of Bifidobacterium and Lactobacillus Relieve Constipation Induced by Loperamide in Mice

Nutrients, Год журнала: 2023, Номер 15(19), С. 4230 - 4230

Опубликована: Сен. 30, 2023

Constipation is currently one of the most common gastrointestinal disorders, and its causes are diverse. Multi-strain probiotics often considered a more effective treatment than single-strain probiotics. In this study, constipation model was constructed using loperamide hydrochloride to evaluate ability multi-strain probiotic combination four different ratios Bifidobacterium Lactobacillus regulate intestinal flora, relieve constipation, explore initial mechanism in mice. After weeks intervention, BM1, BM2, PB2 effectively relieved constipation; however, pathways involved were different. The Bifidobacteria-dominated formulations BM1 BM2 mainly changed composition structure flora significantly decreased relative abundance Tyzzerella, Enterorhabdus, Faecalibaculum, Gordonibacter, Mucispirillum stool; increased Parabacteroides content short-chain fatty acids (SCFAs) restored motilin (MTL) vasoactive peptide (VIP) levels; downregulated interleukin 6 (IL-6) IL-8 levels serum. This repaired inflammatory response caused by constipation. Finally, it promoted peristalsis tract, increasing stool water content, relieving While Lactobacillus-dominated formula serum neurotransmitters (MTL, SP (substance P), VIP PYY (Peptide YY)) factors (IL-1, IL-6 IL-8), Gordonibacter then acetic acid thereby reducing level inflammation changing properties motility.

Язык: Английский

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Multistrain Probiotics Alleviate Diarrhea by Modulating Microbiome-Derived Metabolites and Serotonin Pathway

Probiotics and Antimicrobial Proteins, Год журнала: 2024, Номер unknown

Опубликована: Март 12, 2024

Abstract Diarrhea, a common gastrointestinal symptom in health problems, is highly associated with gut dysbiosis. The purpose of this study to demonstrate the effect multistrain probiotics (Sensi-Biome) on diarrhea from perspective microbiome-neuron axis. Sensi-Biome ( Lactiplantibacillus plantarum , Bifidobacterium animalis subsp. lactis Lactobacillus acidophilus Streptococcus thermophilus bifidum and Lactococcus ) was administered 4% acetic acid–induced rat model at concentrations 1 × 10 8 (G1), 9 (G2), CFU/0.5 mL (G3). Diarrhea-related parameters, inflammation-related cytokines, stool microbiota analysis by 16S rRNA were evaluated. A targeted untargeted metabolomics approach used analyze cecum samples using liquid chromatography orbitrap mass spectrometry. moisture content p < 0.001), intestinal movement rate 0.05), pH 0.05) significantly recovered G3. Serotonin levels decreased groups. inflammatory serotonin, tryptophan hydroxylase expression improved At phylum level, showed highest relative abundance Firmicutes . Short-chain fatty acids including butyrate, iso-butyrate, propionate, iso-valeric acid modified Equol oleamide In conclusion, effectively controls modulating metabolites serotonin pathway.

Язык: Английский

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Interrelationship between altered metabolites and the gut microbiota in people living with HIV with different immune responses to antiretroviral therapy

Current Research in Microbial Sciences, Год журнала: 2025, Номер 8, С. 100340 - 100340

Опубликована: Янв. 1, 2025

Antiretroviral therapy (ART) effectively reduces opportunistic infections and mortality in people living with HIV (PLWH); however, some patients exhibit poor immune recovery. This study explores the connections among responses, metabolites, gut microbiota PLWH differing reactions to ART. We analyzed composition, markers 38 who showed an immunological response (IR) 32 did not (INR), as classified according CD4+ T-cell levels after 24 months of Additionally, vitro assays using cell counting kit 8, flow cytometry, quantitative real-time reverse transcription PCR were employed assess effects metabolites on viability, marker expression, cytokine levels. Gut metabolic profiles differed significantly between IR INR groups. Enterococcus was more abundant group, whereas [Ruminococcus]_gnavus_group reduced. Significant pathway alterations included decreased folate biosynthesis biotin metabolism. observed negative associations Parabacteroides activation T-cells, positive correlations CD4/CD8 ratios. inverse relationships these markers. Indole-3-acetyl-beta-1-D-glucoside (area under curve value = 0.8931), had best discriminatory ability. Further experiments that proportions CD4+CD57+, effector CD4+, CD4+PD1+, CD8+CD57+, CD8+, CD8+HLA-DR+ T cells. Moreover, mRNA expression analysis treatment led a suppression pro-inflammatory cytokines. The multi-omics approach highlighted potential biomarkers for recovery HIV, suggesting avenues further research into strategies.

Язык: Английский

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Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation

World Journal of Gastroenterology, Год журнала: 2025, Номер 31(9)

Опубликована: Фев. 18, 2025

Runchangningshen paste (RCNSP) is a made of four medicinal and edible homologous Chinese medicine mixed with honey. It known for its ability to nourish yin blood as well loosen the bowel relieve constipation. The pathophysiology functional constipation (FC) associated reduction in mucin-2 (MUC2) secretion microbial dysbiosis. To investigate underlying mechanism RCNSP against FC through MUC2 gut mucosal microbiota. Ultra-performance liquid chromatography tandem mass spectrometry characterized composition elucidate material basis action. model was induced via loperamide gavage (16 mg/kg) twice daily 7 days. Applying defecation function gastrointestinal motility assess severity. Hematoxylin eosin Alcian blue-periodic acid-schiff staining analyzed colonic morphology. Transmission electron microscope used observe ultrastructure goblet cells (GCs). Immunofluorescence colocalization, quantitative PCR, western blot assessed impact on gene protein expression within NLRP6/autophagy pathway. 16S rDNA employed sequence contained 12 components potential laxative effects. enhanced function, accelerated motility, maintained integrity. treatment significantly increased GC abundance production while preserving ultrastructure. At molecular level, colocalized key regulatory proteins modulated mRNA expressions Through sequencing analysis, altered microbiota composition. Specifically, it beneficial bacterial strains reducing harmful ones. Simultaneously, reduced butyrate-producing bacteria like Proteobacteria, Enterobacteriaceae, Blautia, Eubacterium decreased hydrogen sulfide-producing species, such Prevotellaceae. also bile acid-inhibiting g_Eubacter_coprostanoligenes_group Erysipelotrichaceae increasing acid-producing Colidextribacter. Our findings suggested that ameliorated dual mechanism: stimulated by activating NLRP6 inflammasome-mediated autophagy

Язык: Английский

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Therapeutic Mechanism of Zhuyang Tongbian Decoction in Treating Functional Constipation: Insights from a Pilot Study Utilizing 16S rRNA Sequencing, Metagenomics, and Metabolomics

International Journal of General Medicine, Год журнала: 2025, Номер Volume 18, С. 1007 - 1022

Опубликована: Фев. 1, 2025

To explore the mechanism of Zhuyang Tongbian Decoction (ZTD) in treating functional constipation (FC) by observing its effects on intestinal flora composition, metabolic function gut microbiota, fecal short-chain fatty acid (SCFA) levels, and serum concentrations TLR4, NF-κB, TNF-α, IL-6 patients with FC. 40 FC were randomly divided into control group treatment group, 20 cases each group. And healthy volunteers recruited during same period. The was administered lactulose, while treated ZTD. 16s RNA sequencing technology used to compare changes structure diversity before after treatment. Changes levels SCFAs faeces TNF-α analysed. Metagenomics assessed microbiota functions. showed a significant increase relative abundance beneficial bacteria, including Bifidobacterium, Lactobacillus, Faecalibacterium_prausnitzii (P < 0.05), whereas Desulfobacterota Ruminococcus significantly reduced 0.05). Notably, acetic propionic higher Serum biomarkers decreased that Carbohydrate metabolism, Metabolism cofactors vitamins, C5- Branched dibasic metabolism increased ZTD notably improves composition function, regulates SCFA reduces inflammation markers patients. strain shows potential regulation may play crucial role efficacy for

Язык: Английский

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The microbiome and the gut-lung axis in tuberculosis: interplay in the course of disease and treatment

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Окт. 31, 2023

Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis (MTB) that remains significant global health challenge. The extensive use of antibiotics in treatment, disrupts the delicate balance microbiota various organs, including gastrointestinal and respiratory systems. This gut-lung axis involves dynamic interactions among immune cells, microbiota, signaling molecules from both organs. alterations microbiome resulting anti-TB treatment can significantly influence course tuberculosis, impacting aspects such as complete healing, reinfection, relapse. review aims to provide comprehensive understanding context with specific focus on impact microbiome.

Язык: Английский

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Insights into the Current and Possible Future Use of Opioid Antagonists in Relation to Opioid-Induced Constipation and Dysbiosis

Molecules, Год журнала: 2023, Номер 28(23), С. 7766 - 7766

Опубликована: Ноя. 24, 2023

Opioid receptor agonists, particularly those that activate µ-opioid receptors (MORs), are essential analgesic agents for acute or chronic mild to severe pain treatment. However, their use has raised concerns including, among others, intestinal dysbiosis. In addition, growing data on constipation-evoked dysbiosis have been reported. Opioid-induced constipation (OIC) creates an obstacle continuing treatment with opioid analgesics. When non-opioid therapies fail overcome the OIC, antagonists peripheral, fast first-pass metabolism, and gastrointestinal localized effects remain drug of choice which discussed here. At first glance, seems only be restricted constipation, however, recent OIC-related its contribution appearance several side garnered a great attention from researchers. Peripheral MORs also considered as future target analgesics limited central effects. The properties MOR counteracting influence possibly peripheral MOR-mediated antinociception, will highlighted. A new concept is proposed developing gut-selective treat restore OIC while keeping antinociception unaffected. impact in relation changes gut microbiome included.

Язык: Английский

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Personalized probiotic strategy considering bowel habits: impacts on gut microbiota composition and alleviation of gastrointestinal symptoms via Consti-Biome and Sensi-Biome

Frontiers in Nutrition, Год журнала: 2024, Номер 11

Опубликована: Фев. 16, 2024

Personalized probiotic regimens, taking into account individual characteristics such as stool patterns, have the potential to alleviate gastrointestinal disorders and improve gut health while avoiding variability exhibited among individuals by conventional probiotics. This study aimed explore efficacy of personalized interventions in managing distinct patterns (constipation diarrhea) investigating their impact on microbiome symptoms using a prospective, randomized, double-blind, placebo-controlled clinical trial design. research leverages multi-strain formulas, Consti-Biome Sensi-Biome, which previously demonstrated alleviating constipation diarrhea symptoms, respectively. Improvement improvement compositional changes were analyzed participants with predominant or symptoms. Results indicate that tailored probiotics could promoting

Язык: Английский

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What We Know About the Actual Role of Traditional Probiotics in Health and Disease

Probiotics and Antimicrobial Proteins, Год журнала: 2024, Номер 16(5), С. 1836 - 1856

Опубликована: Май 3, 2024

Язык: Английский

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